發(fā)布時(shí)間：2022-11-09 21:22

　　紅曲霉（Monascus）屬微生物菌種可合成有用的次生代謝產(chǎn)物紅曲色素（MPs）,它們作為食品色素被廣泛應(yīng)用于食品工業(yè)中。此外,MPs在醫(yī)藥、化妝品和紡織行業(yè)中也具有良好的市場(chǎng)前景。傳統(tǒng)的紅曲色素的生產(chǎn)主要采用固態(tài)發(fā)酵方法,但是固態(tài)發(fā)酵在規(guī)�；糯笊a(chǎn)中還存在一定的局限性。相比于固態(tài)發(fā)酵,采用液態(tài)發(fā)酵技術(shù)生產(chǎn)紅曲色素在工業(yè)規(guī)模上更具有優(yōu)勢(shì)和競(jìng)爭(zhēng)力。本論文以從市售紅曲米粉中分離篩選的一株紫色紅曲霉（Monascus purpureus）為發(fā)酵菌種,研究了不同碳源、氮源等營(yíng)養(yǎng)物質(zhì)及其濃度,有機(jī)酸代謝調(diào)節(jié)劑,不同光照條件對(duì)該菌株液態(tài)發(fā)酵產(chǎn)紅曲色素的影響,并初步優(yōu)化了發(fā)酵培養(yǎng)基組成和發(fā)酵條件,旨在為MPs的液態(tài)發(fā)酵生產(chǎn)提供參考。主要研究結(jié)果如下:（1）影響MPs合成的營(yíng)養(yǎng)物質(zhì)種類和濃度的單因素實(shí)驗(yàn)結(jié)果表明:以葡萄糖為唯一碳源時(shí)可獲得較高的菌絲體濃度和MPs產(chǎn)量,以甘油為唯一碳源時(shí)可促進(jìn)MPs的合成,但是對(duì)菌體的合成影響不顯著,葡萄糖和甘油的適宜濃度分別為120 g/L和60 g/L;適宜的氮源為KNO₃和蛋白胨,適宜濃度分別為2 g/L和10g/L;KH₂

【文章頁數(shù)】：78 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
Abstract
Chapter1 Literature review
    1.1 Introduction
    1.2 Production of MPs and its influential factors
        1.2.1 Production of MPs
        1.2.2 Influential factors of pigments production
    1.3 Biosynthetic pathway and composition of MPs
        1.3.1 Biosynthetic pathway of MPs
        1.3.2 Composition of MPs
    1.4 Bioactivity of MPs
        1.4.1 Antiobesity effect
        1.4.2 Anticancer activity
        1.4.3 Anti-inflammatory activities
        1.4.4 Antidepressant effect
        1.4.5 Anti-osteoporosis effect
        1.4.6 Anti-diabetic effect
    1.5 Application of MPs
        1.5.1 Pharmaceutical utilization
        1.5.2 Food industry
        1.5.3 Uses of MPs in the dye industries
    1.6 Problem statement,aims and objectives
        1.6.1 Problem statement
        1.6.2 Aims and objectives
Chapter2 Materials and methods
    2.1 Chemical reagents and experimental instruments
        2.1.1 Chemical reagents
        2.1.2 Experimental instruments
    2.2 Microorganism and seed culture conditions
    2.3 Experiment design
        2.3.1 Effects of different nutrients on the MPs production by M.purpureus
        2.3.2 The orthogonal experimental design
        2.3.3 Effect of illumination on the growth and MPs production of M.purpureus
        2.3.4 Effect of culture conditions on the growth and MPs production of M.purpureus
    2.4 Analytical method
        2.4.1 Determination of biomass
        2.4.2 Quantification of pigments
    2.5 Statistical analysis
Chapter3 Results
    3.1 Effects of various carbon sources and concentrations on the MPs production by M.purpureus
        3.1.1 Effects of different types of carbon sources
        3.1.2 Effects of glucose and glycerol concentrations
    3.2 Effects of different nitrogen sources and concentrations on the MPs production byM.purpureus
        3.2.1 Effects of different types of nitrogen sources
        3.2.2 Effects of peptone and KNO3 concentrations
    3.3 Effects of different concentration of inorganic salts and organic acids on the MPsand biomass production by M.purpureus
        3.3.1 Effects of inorganic salts
        3.3.2 Effects of organic acids
    3.4 Optimization of the medium composition for MPs production by OED
        3.4.1 Orthogonal experiment result
        3.4.2 ANOVA analysis
    3.5 Effects of different cultural conditions on the MPs production by M.purpureus
        3.5.1 pH value
        3.5.2 Volume of medium
        3.5.3 Culture temperature
        3.5.4 Shaking speed
    3.6 Effects of different illumination on the MPs production by M.purpureus
        3.6.1 Effects of different light source and intensity on MPs production
        3.6.2 Effects of the different light source on the morphology of M.purpureus
        3.6.3 The biomass and MPs production under optimized conditions
Chapter4 Discussion
    4.1 Effects of various carbon sources and concentrations
    4.2 Effects of different nitrogen sources and concentrations
    4.3 Effects of different concentration of inorganic salts and organic acids
    4.4 Optimization of the medium composition for MPs production by OED
    4.5 Effects of different cultural conditions
    4.6 Effects of different illumination conditions
    4.7 The biomass and MPs production under optimized conditions
Chapter5 Conclusions and prospects
    5.1 Conclusions
    5.2 Prospects
References
Research achievements during postgraduate study
Acknowledgements


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期刊論文
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