本研究主要分為兩個部分內(nèi)容,第一部分重點介紹了MYB家族相關(guān)轉(zhuǎn)錄因子的克隆,而第二部分闡述了鹽度對毛竹（Phyllostachysedulis）植物的影響。MYB家族轉(zhuǎn)錄因子在植物生長發(fā)育過程中起著重要作用,特別是在植物抗非生物脅迫以及植物生理結(jié)構(gòu)改良方面。然而由于基因組尚未測序,因此從慈竹中克隆MYB相關(guān)基因并分析其在抗逆方面的功能是一項比較艱巨的任務(wù)。中國竹資源豐富,特別是四川,云南和福建省覆蓋面積較大。竹子是一種重要的可再生木材資源,具有快速生長,多功能性以及高經(jīng)濟價值等特點,含有70%或更高含量的優(yōu)質(zhì)纖維,是造紙的良好原料。Bambusa emeiensis是重要的造紙竹種之一,適應能力強,能夠在惡劣的環(huán)境條件下成功存活。竹子生長迅速且能適應各種嚴酷的環(huán)境,通過克隆和鑒定與其相關(guān)的MYB轉(zhuǎn)錄因子并遺傳轉(zhuǎn)化小麥和煙草,為進一步提高小麥和煙草抗逆能力提供了依據(jù)。我們已成功克隆了兩個MYB家族的為“BeMYB680”和“BeMYB140”。通過生物信息學分析,酵母單雜交,亞細胞定位和表達分析確定其為MYB家族基因。最后,構(gòu)建過表達載體,通過基因槍和農(nóng)桿菌轉(zhuǎn)化為小麥（基因型為“DN77... 

【文章頁數(shù)】：137 頁

【學位級別】：博士

【文章目錄】：
摘要
abstract
1 Introduction
    1.1 Research significance
        1.1.1 Bamboo Description
        1.1.2 Uses of Bamboo
        1.1.3 Description of wheat
        1.1.4 Tobacco
    1.2 Research Background
        1.2.1 Transcription factors
        1.2.2 Pathway for Transcriptional Factors Response
        1.2.3 Abiotic stresses overview
        1.2.4 Drought
        1.2.5 Salinity
        1.2.6 Lignin contents in plants
    1.3 Research objectives and Technical route
        1.3.1 Research Objectives
        1.3.2 Technical Route
2 Study on the cloning, bioinformatics analysis, transcriptional activation andSubcellular localization of BeMYB140 and BeMYB680
    2.1 Cloning and bioinformatics analysis of BeMYB140 and BeMYB680
        2.1.1 Materials
        2.1.2 Chemicals of TaKara Dalian, China
        2.1.3 Other chemicals includes
        2.1.4 Equipment's
        2.1.5 Methodology
        2.1.6 Results
    2.2 Analysis of transcriptional activation of BeMYB140 & BeMYB680
        2.2.1 Materials and Equipment's
        2.2.2 Methodology
        2.2.3 Results
    2.3 Essay of Sub-Cellular localization for BeMYB140 & BeMYB680
        2.3.1 Materials and Equipment's
        2.3.2 Methodology
        2.3.3 Results
    2.4 The expression profiling of BeMYB140 & BeMYB680
        2.4.1 Materials and Equipment's
        2.4.2 Methodology
        2.4.3 Results
3 Study on the transformation of BeMYB140 and BeMYB680
    3.1 Transformation of recombinant plasmid into Wheat and tobacco
        3.1.1 Materials and Equipment's
        3.1.2 Methodology
        3.1.3 Results
    3.2 Discussion and Conclusion
        3.2.1 Discussion
        3.2.2 Conclusion
4 Effect of Salinity on Phyllostachys edulis
    4.1 Methodology
        4.1.1 Layout of experiment
        4.1.2 Experimental design
        4.1.3 Sample collection
        4.1.4 Physiological parameters analysis
        4.1.5 Enzymatic Activity
        4.1.6 Expression profiling of BeMYH 140, BeMYB680, BeSNAC1 and BeWRKY2
        4.1.7 Analysis of Minerals contents in leaves of Phyllostachys edulis
    4.2 Results
        4.2.1 Physical properties
        4.2.2 Enzymatic properties
        4.2.3 Transport of salts from the soil on to the leaves of Phyllostachys edulis
        4.2.4 Expression profiling of Cloned genes in response to salinity
        4.2.5 Minerals contents
    4.3 Discussion and Conclusion
        4.3.1 Dscussion
        4.3.2 Conclusion
5 Acknowledgement
6 Reference
Appendix
Academic papers published and research findings related to the content of dissertations



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