目的多種生理和病理狀態(tài)都會(huì)伴隨著慢性缺氧,例如紫紺型先天性心臟�。–CHD）。這種慢性缺氧可能會(huì)干擾基因的轉(zhuǎn)錄過(guò)程。然而,在缺氧條件下外周血轉(zhuǎn)錄組模式的改變尚無(wú)報(bào)道。故本工作旨在探討慢性缺氧條件下,外周血轉(zhuǎn)錄組模式的變化。方法本研究使用慢性缺氧大鼠模型模擬CCHD患者的低氧狀態(tài)。兩組Sprague-Dawley大鼠（每組n=6）分別暴露于低氧（10%O2）或常氧（21%O2）條件下飼養(yǎng)3周。每周測(cè)量大鼠體重。缺氧處理結(jié)束后,采集兩組大鼠外周血并提取總RNA進(jìn)行RNA-Seq。經(jīng)過(guò)質(zhì)量評(píng)估后,通過(guò)Illumina Hiseq平臺(tái)對(duì)文庫(kù)進(jìn)行測(cè)序。篩選差異表達(dá)基因（DEG）,篩選條件為FDR（false discovery rate）<0.05且FC（fold change）>2。進(jìn)行DEG的功能注釋和聚類分析,并選取出padj（adjusted P-value）<0.05的條目。結(jié)果低氧組大鼠體重較對(duì)照組明顯降低（P<0.01）。RNA-Seq結(jié)果表明:兩組的轉(zhuǎn)錄組模式有顯著差異。共鑒定出了872個(gè)差異表達(dá)的基因。在缺氧組中,共有803個(gè)基因下調(diào),只有69個(gè)基因上調(diào)... 

【文章來(lái)源】：Chinese Medical Sciences Journal. 2020,35(01)CSCD

【文章頁(yè)數(shù)】：12 頁(yè)

【文章目錄】：
MATERIALS AND METHODS
    Study design and animal model
    Blood sampling and RNA extraction
    RNA library preparation and deep sequencing
    Quality control and reads mapping
    Quantification of gene expression level and differential expression analysis
    Functional annotation and enrichment of differentially expressed genes
    Protein-Protein Interaction analysis of DEGs
    Statistical analysis
RESULTS
    The characteristics of the studied rats
    Quality of RNA-Seq analysis
    Differential gene expression analysis between two groups
    Functional enrichment analysis of DEGs
    Gene interaction network and functional modules
DISCUSSION
Conflict of Interests Statement



本文編號(hào)：3240432