本研究旨在探討心臟成纖維細(xì)胞旁分泌能力對小鼠胚胎干細(xì)胞的影響。新生小鼠和成年小鼠成纖維細(xì)胞的條件培養(yǎng)基（ConM-NCF、ConM-ACF）分別稀釋50倍（1:50）或5倍（1:5）,通過RT-real time PCR技術(shù)、ELISA、細(xì)胞增殖檢測以及計(jì)數(shù)心肌分化過程中跳動擬胚體（embryoid body, EB）的比例,觀察這些條件培養(yǎng)基對小鼠胚胎干細(xì)胞的影響。結(jié)果顯示,成纖維細(xì)胞細(xì)胞因子的分泌能力在發(fā)育過程中發(fā)生顯著變化,其中白細(xì)胞介素6 （interleukin 6, IL6）隨發(fā)育成熟顯著增加。ConM-NCF抑制干細(xì)胞的增殖,但高、低濃度對干性基因表達(dá)的作用不同。Con M-ACF 1:50促進(jìn)干細(xì)胞增殖并上調(diào)干性基因,但Con M-ACF1:5作用相反。ConM-NCF和ConM-ACF都能抑制干細(xì)胞的心肌細(xì)胞分化能力,但又各有特點(diǎn):ConM-NCF 1:50抑制早期心肌分化但不影響晚期心肌細(xì)胞標(biāo)記物的表達(dá);ConM-ACF 1:50抑制早期心肌分化比ConM-NCF 1:50更顯著,阻礙心肌成熟并上調(diào)分化晚期的心肌細(xì)胞標(biāo)記物表達(dá)水平。此外,IL6中和抗體抑制ConM-... 

【文章來源】：生理學(xué)報. 2020,72(05)北大核心CSCD

【文章頁數(shù)】：9 頁

【文章目錄】：
1 MATERIALS AND METHODS
    1.1 CFs isolation and culture
    1.2 Mouse ESCs culture and cardiomyocyte differentiation
    1.3 Cell proliferation assay
    1.4 RT-real time PCR
    1.5 Enzyme linked immunosorbent assay （ELISA）
    1.6 Statistics
2 RESULTS
    2.1 Con M-NCF and Con M-ACF functioned dis-tinctly in mouse ESC proliferation and pluripotency markers expression
    2.2 Con M-NCF and Con M-ACF distinguishably changed the cardiomyocyte differentiation of mouse ESCs
    2.3 CFs-secreted growth factors and cytokines changed during development
    2.4 IL6 dominated partial functions of the Con Ms derived from CFs in mouse ESCs
3 DISCUSSION


【參考文獻(xiàn)】：
期刊論文
[1]Thymosin β4 Impeded Murine Stem Cell Proliferation with an Intact Cardiovascular Differentiation[J]. 聶麗,高仕君,趙亞楠,Jacob Masika,駱紅艷,胡新武,張亮品,曾瑩,Jürgen Hescheler,梁華敏.  Journal of Huazhong University of Science and Technology（Medical Sciences）. 2016(03)



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