槐米是豆科植物國(guó)槐的干燥花蕾,廣泛栽培于中國(guó)的各個(gè)省市、日本和韓國(guó)�；泵资且环N珍貴的中草藥,具有清熱、涼血和止血作用。本試驗(yàn)以產(chǎn)自山西省夏縣的槐米為試驗(yàn)原料,研究了槐米黃酮類化合物的提取工藝,槐米中多種黃酮物質(zhì)的測(cè)定方法及其抗氧化性能。本文研究結(jié)果如下:本研究建立了定性和定量分析槐米提取物中六種黃酮化合物的高效液相色譜方法。實(shí)驗(yàn)條件為:流動(dòng)相由0.1%磷酸水溶液（A）和乙腈（B）組成。使用前用45μm微孔濾膜過(guò)濾,并超聲脫氣。色譜柱Diamonsil TMC18柱（250mm×4.6mm,5μm）,柱溫為30℃,進(jìn)樣量為5μL,流速為1.0 mL/min。目標(biāo)組分的檢測(cè)波長(zhǎng)為360 nm。流動(dòng)相梯度洗脫條件如下:0-30 min,95-65%A;30-35min,65-95%A;35-50 min,95%A。結(jié)果表明該方法準(zhǔn)確、有效,可用于槐米黃酮物質(zhì)的質(zhì)量控制。采用超聲輔助提取槐米中的蘆丁、煙草苷、水仙苷、山奈酚、異鼠李素、槲皮素和總黃酮。通過(guò)單因素試驗(yàn)和響應(yīng)面法確定了最佳提取條件為:乙醇濃度為70%,時(shí)間30 min,溫度61℃,液固比15.30mL/g。蘆丁、煙草苷、水仙苷、山奈... 

【文章來(lái)源】：山西大學(xué)山西省

【文章頁(yè)數(shù)】：93 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
Abstract
Chapter 1 Review of the literature
    1.1 The overview of Flos Sophorae Immaturus
        1.1.1 The profile of Flos Sophorae Immaturus
        1.1.2 Nutrient composition analysis of FSI
        1.1.3 The utilization value of FSI
    1.2 Overview of flavonoids from FSI
        1.2.1 Pharmacology activities
        1.2.2 Determination of flavonoids from FSI
        1.2.3 Extraction methods of flavonoids from FSI
        1.2.4 Purification of flavonoids from FSI
    1.3 Research content, research purpose and research significance
        1.3.1 Research content
        1.3.2 Research purpose
        1.3.3 Research significance
Chapter 2 Determination of the six flavonoids in Flos Sophorae Immaturus byHPLC
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Materials and reagents
        2.2.2 HPLC conditions
        2.2.3 Preparation of standard stock solution
        2.2.4 Preparation of sample solution
        2.2.5 Validation
        2.2.6 Statistical analysis
    2.3 Results and discussion
        2.3.1 Chromatographic profile
        2.3.2 Validation of the HPLC method for FSI extract
        2.3.3 Specificity/Selectivity
        2.3.4 Linearity, LOD and LOQ
        2.3.5 Precision
        2.3.6 Stability
        2.3.7 Repeatability
        2.3.8 Accuracy/Recovery assay
        2.3.9 Sample determination
    2.4 Summary
Chapter 3 Optimization of Ultrasound-Assisted Extraction for SimultaneousQuantitation of Six Flavonoids in Flos Sophorae Immaturus
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Sample of FSI
        3.2.2 Chemicals and Apparatus
        3.2.3 Preparation of Standard Solution
        3.2.4 Determination of TFFSI by UV
        3.2.5 Determination of six main flavonoids by HPLC
        3.2.6 Sample Preparation Based on UAE
        3.2.7 Experimental Design
        3.2.8 Statistical analysis
    3.3 Results and discussion
        3.3.1 Univariate Experiments Design and Analysis
        3.3.2 Multi-Response Design and Analysis
    3.4 Summary
Chapter 4 Antioxidant activity of Flavonoids in Flos Sophorae Immaturus
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Chemicals and Apparatus
        4.2.2 Sample Preparation
        4.2.3 Measurement of scavenging activity on DPPH radicals
        4.2.4 Measurement of scavenging activity on ABTS·+
        4.2.5 Superoxide anion scavenging activity (SASA)
        4.2.6 Ferric reducing antioxidant power (FRAP)
        4.2.7 Statistical analysis
    4.3 Results and discussion
        4.3.1 Scavenging activity of DPPH radical
        4.3.2 Scavenging activity of ABTS·+
        4.3.3 Scavenging activity of Superoxide anion (·O_2~-) radical
        4.3.4 Ferric reducing antioxidant power (FRAP)
    4.4 Summary
Chapter 5 Conclusion and Prospect
    5.1 Conclusion
    5.2 Prospect
References
攻讀學(xué)位期間取得的研究成果
致謝
個(gè)人簡(jiǎn)況及聯(lián)系方式



本文編號(hào)：3283015