本試驗(yàn)研究了日糧中的葉酸對(duì)團(tuán)頭魴(Megalobramaamblycephala)生長(zhǎng)、生理生化以及急性高溫應(yīng)激下生理生化的影響。團(tuán)頭魴初體重27.0±0.03g,試驗(yàn)配置6組半純合日糧,分別含葉酸0.0、0.5、1.0、2.0、5.0和10.0mg/kg飼料,養(yǎng)殖試驗(yàn)在循環(huán)水系統(tǒng)中進(jìn)行,周期為10周,每組飼料3個(gè)平行。研究結(jié)果顯示,飼喂1.0、2.0和5.0 mg/kg葉酸組團(tuán)頭魴的終末重、增重率和特定生長(zhǎng)率顯著高于其他兩組(P<0.05)。另外,1.0mg/kg組的顯著影響團(tuán)頭魴的餌料系數(shù)和肥滿度。飼喂1.0mg/kg和2.0mg/kg葉酸組的團(tuán)頭魴的肝體比和臟體比顯著增加。葉酸對(duì)團(tuán)頭魴肌肉水分、(?)分和粗脂肪含量影響不顯著(P>0.05)。但是顯著影響肌肉粗蛋白含量,1.0mg/kg和2.0mg/kg葉酸組的團(tuán)頭魴的肌肉粗蛋白含量顯著高于對(duì)照組和0.5mg/kg葉酸組(P<0.05),但與其他各組差異不顯著。根據(jù)增重率、特定生長(zhǎng)率和肝體比,團(tuán)頭魴日糧中最適葉酸含量分別為0.68mg/kg、0.68mg/kg和0.82mg/kg。日糧中的葉酸含量對(duì)團(tuán)頭魴血液中的... 

【文章來源】：南京農(nóng)業(yè)大學(xué)江蘇省 211工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：91 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
ABSTRACT
摘要
CHAPTER 1 Literature Review and Study Plan
    1.1 Dietary folic acid
        1.1.1 Functions of Folic Acid
    1.2 Fish Specific Stressors
        1.2.1 Capture, Transport, and Handling
        1.2.2 Contaminants
        1.2.3 Crowding
    1.3 Stress Responses and organ system
        1.3.1 Gills
        1.3.2 Liver
        1.3.3 Integument
    1.4 An overview of the nutrition of M. amblycephala
    1.5 Aim of the study
    1.6 Hypothesis of the study
CHAPTER 2 Effect of dietary Folic acid on growth performance and muscle chemical composition ofblunt snout bream M. amblycephala fingerling
    1 Introduction
    2 Materials and Methods
        2.1 Formulation and preparation of experimental diets
        2.2 Research facility and fish rearing
        2.3 Sampling and fish weighing
        2.4 Calculations
        2.5 Proximate composition analysis
        2.6 Statistical analysis
    3 Results
        3.1 Growth performance and feed utilization
        3.2 Effect of dietary folic acid level on the muscle proximate chemical composition
    4 Discussion
        4.1 Growth performance and feed utilization
        4.2 Effect of dietary folic acid level on the muscle proximate chemical composition
    5 Conclusion
CHAPTER 3 Effect of dietary Folic acid on blood hematological parameters and Digestive enzymesactivities of blunt snout bream fingerling M. amblycephala
    1 Introduction
    2 Materials and Method
        2.1 Formulation and preparation of experimental diets
        2.2 Research facility and fish rearing
        2.3 Sample collection
        2.4 Effect of dietary folic acid on Hematological measurements
        2.5 Effect of dietary folic acid on digestive enzyme activity
        2.6 Statistical analysis
    3 Results
        3.1 Effect of dietary folic acid on blood Hematological parameters
        3.2 Effect of dietary folic acid on digestive enzyme activity
    4 Discussion
        4.1 Effect of dietary folic acid on blood hematology
        4.2 Effect of dietary folic acid on digestive enzyme activity
    5 Conclusion
CHAPTER 4 Effect of dietary folic acid on biochemical parameters, Antioxidant enzymes activities andgene expression of three heat shock proteins （HSPs） in blunt snout bream Megalobrama amblycephalafingerling under acute high temperature stress
    1 Introduction
    2 Materials and Method
        2.1 Formulation and preparation of experimental diets
        2.2 Research facility and fish rearing
        2.3 Collection of Sample
        2.4 Biochemical measurements
        2.5 Immune and antioxidant parameters
        2.6 Real-time PCR analysis of hepatic HSP60, HSP70 and HSP90
        2.7 Statistical analysis
    3 Results
        3.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge
        3.2 Effect of dietary folic acid level on the serum Cortisol, Glucose, ACTH, ALT, AST andALP under acute high temperature challenge
        3.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperaturechallenge
        3.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge
        3.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge
        3.6 Effect of dietary folic acid on gene expression of three heat shock proteins （HSPs） underacute high temperature stress
    4 Discussion
        4.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge
        4.2 Effect of dietary folic acid level on the serum Glucose, Cortisol, ACTH, ALT, AST andALP under acute high temperature challenge
        4.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperatureStress
        4.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge
        4.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge
        4.6 Effect of dietary folic acid on gene expression of three heat shock proteins （HSPs） underacute high temperature stress
    5 Conclusion
Conclusion of the whole study
References
Appendix
Acknowledgements
Meetings attended and publications
Awards


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