獨腳金內(nèi)酯（SLs）是一類新發(fā)現(xiàn)的植物激素,廣泛參與植物的生長發(fā)育,并參與植物根系與土壤微生物的互作。獨腳金內(nèi)酯類化合物能夠刺激叢枝菌根（AMF）菌絲的分枝和促進根寄生植物的種子萌發(fā)。豆科植物,如蒺藜苜蓿（Medicago truncatula）能夠與根瘤菌形成植物-細菌互惠共生關系,通過根瘤這一特殊的器官進行固氮作用。獨腳金內(nèi)酯能夠調控根系生長,莖的分枝,植物-微生物共生體的產(chǎn)生,同時還能夠響應缺磷和各種逆境脅迫。盡管最近在模式植物中對獨腳金內(nèi)酯進行了大量的研究,然而大豆中獨腳金內(nèi)酯的生物合成及信號傳導研究還很少。本研究中我們克隆了大豆獨腳金內(nèi)酯合成酶基因和信號傳導基因。我們研究了大豆GmMAX1,GmMAX2,GmMAX3和GmMAX4,以及GmD14,GmD53,GmD27和GmPDR1等獨腳金內(nèi)酯的合成酶基因和信號傳導基因在不同組織中的表達模式,以及在外界非生物逆境脅迫下的表達。同時我們還檢測了根瘤菌感染后大豆根以及大豆根瘤不同發(fā)育階段的相關基因的表達情況。為了進一步研究GmMAX的基因功能,我們構建了GmMAX1a,GmMAX2a,GmMAX3a和GmMAX4a四個基因的Gm... 

【文章來源】：華中農(nóng)業(yè)大學湖北省 211工程院校 教育部直屬院校

【文章頁數(shù)】：123 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
Abbreviation
CHAPTER I Introduction
    1.1 Strigolactones(SLs):An overview
    1.2 Chemical nature of SLs
    1.3 SLs biosynthesis pathway
    1.4 Strigolactone's signaling pathway
        1.4.1 SLs perception
        1.4.2 SLs signaling
        1.4.3 SLs transports
    1.5 Biological functions of SLs
        1.5.1 SLs signaling in the control of branching
        1.5.2 SLs signaling in root development
        1.5.3 SLs and drought/salt stress
        1.5.4 Role of SLs in leaf senescence
        1.5.5 SLs elongates inter-node length
        1.5.6 Role of SLs in nodulation
    1.6 SLs are signals for plant interactions
        1.6.1 Mycorrhizal symbiosis
        1.6.2 Parasitic plants
        1.6.3 Symbiotic interaction of SLs with rhizobium spp
    1.7 Interaction with auxin
        1.7.1 Interaction with ABA
    1.8 More axillary growth(MAXs)genes and their role in SLs biosynthesis and signaling pathway
        1.8.1 Role of MAX1 in SLs biosynthesis
        1.8.2 Role of MAX2 in SLs signaling
        1.8.3 Role of MAX3(CCD7) &MAX4(CCD8)in SLs biosynthesis
    1.9 Aims of the study:The specific aims are as follow
CHAPTER II Materials and methods
    2.1 Plant materials and growth condition
    2.2 Gene cloning and vector construction
    2.3 Hairy root induction and hormone analysis:
        2.3.1 In vitro hairy root induction
        2.3.2 In-vivo hairy root induction
    2.4 GmMAXs transgenic soybean hairy root nodulation assay
    2.5 Rhizobia infection
    2.6 Generation of transgenic hairy roots or collection of nodules for RNA-seq analysis
    2.7 RNA isolation,cDNA library construction for Illumina deep sequencing
    2.8 Quantitative RT-PCR(qRT-PCR)analysis of gene expression
    2.9 Hormone extraction and analysis
    2.10 Bioinformatics analysis
    2.11 Statistical analysis
    2.12 Protein accession number
CHAPTER III Results
    3.1 Identification of SLs biosynthetic and signaling genes from soybean genome
    3.2 Tissue expression pattern of SLs biosynthesis and signaling genes
    3.3 SLs biosynthesis and signaling gene expression upon B.japonicum(USDA110)infection
    3.4 SLs biosynthesis and signaling gene expression at different stages of nodules
    3.5 Effects of GmMAXsa-knockdown on soybean nodulation
    3.6.Effect of GmMAXsa-knockdown on nodulation signaling pathway genes
    3.7 Effect of GmMAXsa knockdown on plant hormones
    3.8 Effects of GmMAX2a-knockdown on SLs biosynthesis and signaling genes
    3.9 Effect of GmMAX2a-knockdown on auxin biosynthesis genes
    3.10 GmMAX2 regulates a wide range of hormone-related genes and diverse transcription factors
    3.11 Overexpression of auxin biosynthesis gene affects the SLs biosynthesis and signaling genes
    3.12 Abiotic stresses affect the SLs biosynthesis and signaling genes
CHAPTER IV Discussion
    4.1 Soybean genome has conserved SLs biosynthesis and signaling components
    4.2 SLs are involved in soybean-rhizobia interaction and nodule development to senescence
    4.3 SLs biosynthesis is essential for nodulation in soybean
    4.4 GmMAX2-dependent SLs signaling is essential for nodulation in soybean
    4.5 SLs biosynthesis and signaling interacts with other hormone signaling
    4.6 SLs in soybean are involved in abiotic stresses
Conclusion
References
Appendices
    Appendix1
    Appendix2
    Appendix3
Acknowledgement
List of publications



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