籽粒大小相關性狀,包括粒長、粒寬、粒厚等,對于玉米的產(chǎn)量與品質(zhì)表現(xiàn)具有決定性的影響。揭示這些性狀的表型特性,并發(fā)掘相應的QTL,對于玉米高產(chǎn)、穩(wěn)產(chǎn)育種,以及品質(zhì)改良都具有重要的實踐指導意義。本研究以自育的穗位極低材料EHel（extremely low Ear Height）與測序自交系B73雜交所構建的F3分離群體為研究對象,全面鑒定了群體的粒長、粒寬、粒厚,并結合10K的SNP芯片基因型鑒定,開展了玉米極低穗位材料EHel籽粒大小性狀的表型鑒定與QTL定位。主要研究結果如下:1.親本及分離群體籽粒大小性狀的表型特性表型鑒定結果顯示,兩個親本EHel與B73的10-粒長（10-kernel length,KL）與10-粒寬（10-kernel width,KW）在2018與2019之間均表現(xiàn)出極顯著差異（P<0.01）。但是EHel的10-粒厚（10-kernel thickness,KT）在兩年之間差異不顯著（P=0.6788）,而B73的KT在兩年間則差異極顯著（P=0.0098）。親本之間的比較結果表明,在2018與2019兩年間,EHel與B73的KL與KW均存在極顯著... 

【文章來源】：西南大學重慶市 211工程院校 教育部直屬院校

【文章頁數(shù)】：68 頁

【學位級別】：碩士

【文章目錄】：
ABSTRACT
摘要
CHAPTER1
    1.1 GLOBAL MAIZE DISTRIBUTION,PRODUCTION,AND CONSUMPTION
        1.1.1 General overview of maize
        1.1.2 Global maize distribution
        1.1.3 Global maize production
        1.1.4 Global maize consumption
    1.2 YIELD AND KERNEL-RELATED YIELD COMPONENTS OF MAIZE
        1.2.1 Yield and kernel-related yield components of harvesting maize
        1.2.2 Documents focused on maize yield and kernels
        1.2.3 Kernel related traits and their contributions to maize yield performance
    1.3 ADVANCE IN GENETICS OF KERNEL RELATED TRAITS IN MAIZE AND OTHER CROPS
        1.3.1 Genetic dissection of kernel related traits
        1.3.2 Detection of chromosomal regions controlled kernel related traits
        1.3.3 Cloned genes for kernel related traits in maize and other crops
    1.4 MAPPING STRATEGY FOR MARKER-TRAIT ASSOCIATION
        1.4.1 QTL mapping and often-used software
        1.4.2 Association mapping
        1.4.3 Bulked segregation/sample analysis（BSA）
    1.5 HIGH-THROUGHPUT GENOTYPING VIA SNP-BASED CHIPSET
CHAPTER2 MATERIALS AND METHODS
    2.1 PLANT MATERIALS
    2.2 EXPERIMENT DESIGN AND DATA COLLECTING
    2.3 PHENOTYPIC DATA ANALYZING
    2.4 CHIPSET-BASED GENOTYPING
    2.5 SCREENING OF POLYMORPHIC SNPS AND CONSTRUCTION OF LINKAGE MAP
    2.6 QTL MAPPING FOR THREE KERNEL-SIZE RELATED TRAITS
    2.7 VERIFICATION OF CANDIDATE CHROMOSOMAL REGION VIA BULKED STRATEGY
CHAPTER3 RESULTS
    3.1 PHENOTYPIC PERFORMANCE OF THREE KERNEL-SIZE RELATED TRAITS
    3.2 CORRELATION ANALYSIS OF PHENOTYPIC TRAITS
    3.3 CONSTRUCTION OF LINKAGE MAP
    3.4 QTL MAPPING FOR THE TARGET TRAITS
    3.5 PRIMARY VERIFICATION OF TARGET CHROMOSOMAL REGION ON CHR9 VIA BULKED STRATEGY
CHAPTER4 DISCUSSION AND CONCLUSION
    4.1 DISCUSSION
    4.2 CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS



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