Intron-targeted gene insertion strategy using CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9） has been shown to be a potential tool for crop genetic improvement by targeted mutagenesis or gene replacement of an elite allele into widely cultivated rice varieties. The rice blast resistant protein Pi-ta, differs from its susceptible counterpart, pi-ta, by a single amino acid in exon 2. To create new materials resistant to the rice blast disease, we inse... 

【文章來源】：The Crop Journal. 2020,8(03)

【文章頁數(shù)】：8 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Vector construction
    2.2. Rice transformation
    2.3. Molecular characterization of the transgenic plants
3. Results and discussion
    3.1. Intron-targeted insertion of exon 2 of Pi-ta
    3.2. Genotyping of the transgenic lines
    3.3. Selection of the progeny without the transgene
    3.4. Expression analysis of the inserted Pi-ta gene
    3.5. Agronomic trait analysis and detection of potential off-target sites
    3.6. Applications in the molecular breeding
4. Conclusions
Appendix A.Supplementary data


【參考文獻】：
期刊論文
[1]稻瘟病抗病基因Pita和Pib在中國水稻地方品種中的分布[J]. 楊杰,楊金歡,王軍,范方軍,朱金燕,曹卿,田勝尼,仲維功.  華北農(nóng)學(xué)報. 2011(03)



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