Intron-targeted gene insertion strategy using CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9） has been shown to be a potential tool for crop genetic improvement by targeted mutagenesis or gene replacement of an elite allele into widely cultivated rice varieties. The rice blast resistant protein Pi-ta, differs from its susceptible counterpart, pi-ta, by a single amino acid in exon 2. To create new materials resistant to the rice blast disease, we inse... 

【文章來(lái)源】：The Crop Journal. 2020,8(03)

【文章頁(yè)數(shù)】：8 頁(yè)

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Vector construction
    2.2. Rice transformation
    2.3. Molecular characterization of the transgenic plants
3. Results and discussion
    3.1. Intron-targeted insertion of exon 2 of Pi-ta
    3.2. Genotyping of the transgenic lines
    3.3. Selection of the progeny without the transgene
    3.4. Expression analysis of the inserted Pi-ta gene
    3.5. Agronomic trait analysis and detection of potential off-target sites
    3.6. Applications in the molecular breeding
4. Conclusions
Appendix A.Supplementary data


【參考文獻(xiàn)】：
期刊論文
[1]稻瘟病抗病基因Pita和Pib在中國(guó)水稻地方品種中的分布[J]. 楊杰,楊金歡,王軍,范方軍,朱金燕,曹卿,田勝尼,仲維功.  華北農(nóng)學(xué)報(bào). 2011(03)



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