中東亞洲小型1（MEAM1）煙粉虱Bemisia tabaci（Gennadius）（半翅目:粉虱科）,是世界范圍內(nèi)重要的園藝,農(nóng)藝和觀賞作物害蟲(chóng),可以危害六百多種植物,最初被認(rèn)為是B型煙粉虱。植物釋放的揮發(fā)性混合物在食草動(dòng)物選擇合適的宿主植物過(guò)程中發(fā)揮著重要作用。在此研究以前,我們對(duì)植物揮發(fā)物的了解還局限在不同茄科植物（茄子,番茄和甜椒）釋放的揮發(fā)物對(duì)植食性昆蟲(chóng)寄主選擇行為的影響;對(duì)于受草食動(dòng)物損傷誘導(dǎo)的植物揮發(fā)物對(duì)食草動(dòng)物宿主選擇行為的影響也較少有研究。此外,植物的氮濃度會(huì)影響揮發(fā)性有機(jī)化合物（VOC）的排放,而且可能進(jìn)一步影響食草動(dòng)物的侵染行為,但相關(guān)研究相對(duì)較少。在本研究中,我們使用風(fēng)洞和‘Y’型管嗅覺(jué)儀測(cè)定了煙粉虱對(duì)茄子、番茄和甜椒三種茄科植物的反應(yīng)。我們還利用自由選擇和嗅覺(jué)實(shí)驗(yàn)研究了:煙粉虱和桃蚜單獨(dú)或共同取食番茄后,煙粉虱對(duì)番茄植物的侵染行為差異。此外,由于施用不同含量的氮素營(yíng)養(yǎng)后番茄會(huì)產(chǎn)生不同水平的VOC;我們利用風(fēng)洞和嗅覺(jué)儀檢測(cè)了煙粉虱對(duì)上述番茄植物氮素營(yíng)養(yǎng)處理后VOC的響應(yīng)。同時(shí),我們利用氣相色譜質(zhì)譜聯(lián)用儀（GC-MS）分析和量化了的三種茄科植物（茄子、番茄和甜椒）... 

【文章頁(yè)數(shù)】：115 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
ABSTRACT
摘要
CHAPTER Ⅰ GENERAL INTRODUCTION
    1.1 INTRODUCTION
    1.2 IMPORTANCE OF WHITEFLY, BEMISIA TABACI
        1.2.1 Host plants of Bemisia tabaci
        1.2.2 Problems associated to Bemisia tabaci infestation in crop production
        1.2.3 Problems related to the control of Bemisia tabaci
    1.3 VOLATILE ORGANIC COMPOUNDS
        1.3.1 Impact of plant volatiles on herbivorous insects
        1.3.2 Impact of herbivore induced volatiles on the infestation behavior ofphytophagous insects
        1.3.3 Impact of nitrogen on volatile production which influence on host plantselection of herbivorous insects
    1.4 SIGNIFICANCE AND APPLICATION OF THIS STUDY
    1.5 VIEWPOINT AND AIMS OF THIS STUDY
CHAPTER Ⅱ VOLATILE ORGANIC COMPOUNDS OF EGGPLANT, TOMATOAND SWEET PEPPER AFFECT HOST PLANT SELECTION OF BEMISIA TABACI(HEMIPTERA:ALEYRODIDAE)
    2.1 INTRODUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Host plants
        2.2.2 Insect rearing
        2.2.3 Chemicals
        2.2.4 Wind tunnel bioassays
        2.2.5 Olfactory preference test of Bemisia tabaci with Y-tube olfactometer
        2.2.6 Volatile chemicals collection technique from plants
        2.2.7 Analyses of volatile chemicals
        2.2.8 Data analysis
    2.3 RESULTS
        2.3.1 Wind tunnel choice test
        2.3.2 Y-tube olfactometer test
        2.3.3 Volatile organic compounds released from eggplant, tomato and sweet pepper
    2.4 DISCUSSIONS
    2.5 CONCLUSIONS
CHAPTER Ⅲ SINGLE AND MULTIPLE HERBIVORE-INDUCED VOLATILESREDUCE THE ATTRACTIVENESS OF TOMATO PLANTS TO BEMISIA TABACI(HEMIPTERA: ALEYRODIDAE)
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Host plants
        3.2.2 Insect rearing
        3.2.3 Chemicals
        3.2.4 Pre-infestation of host plants
        3.2.5 Free-choice bioassay of Bemisia tabaci with healthy and infested tomato plants
        3.2.6 Y-tube bioassays of Bemisia tabaci responses to olfactory cues
        3.2.7 Repellency synthetic volatile chemicals added on healthy tomato plant toBemisia tabaci
        3.2.8 Volatile chemicals collection procedure
        3.2.9 Analysis of volatile compounds
        3.2.10 Statistical analyses
    3.3 RESULTS
        3.3.1 Free-choice bioassay of Bemisia tabaci among healthy and infested tomatoplants
        3.3.2 Olfactory responses of Bemisia tabaci to healthy and infested tomato plantvolatiles, extracted plant volatiles and synthetic volatile components
        3.3.3 Responses of Bemisia tabaci to synthetic volatile components added on healthytomato plant
        3.3.4 Collection and identification of volatiles
    3.4 DISCUSSION
    3.5 CONCLUSIONS
CHAPTER Ⅳ HIGH LEVEL OF NITROGEN MAKES TOMATO PLANTSRELEASING LESS VOLATILES AND ATTRACTING MORE BEMISIA TABACI(HEMIPTERA:ALEYRODIDAE)
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Plant growth and fertilization
        4.2.2 Insect rearing
        4.2.3 Wind tunnel bioassays
        4.2.4 Olfactory choice test with Y-tube olfactometer
        4.2.5 Determination of nitrogen from tomato plants
        4.2.6 Volatile compound collection from nitrogen applied tomato plants
        4.2.7 Analysis of volatile compounds
        4.2.8 Statistical analyses
    4.3 RESULTS
        4.3.1 Two-way choice tests conducted in a wind tunnel
        4.3.2 Olfactory bioassay with Y-tube olfactometer
        4.3.3 Amounts of nitrogen in tomato plants
        4.3.4 Volatile organic compounds identified from intact tomato plants afterapplication of different levels of nitrogen, quantity of volatile constituents andcorrelation between nitrogen levels
    4.4 DISCUSSION
    4.5 CONCLUSIONS
LITERATURES CITED
ACKNOWLEDGEMENTS
AUTHOR'S PROFILE
LIST OF PUBLICATIONS RELATED TO THIS DEGREE


【參考文獻(xiàn)】：
期刊論文
[1]西花薊馬誘導(dǎo)的番茄植株防御反應(yīng)對(duì)B型煙粉虱行為的影響[J]. 李曉星,酈衛(wèi)弟,呂要斌.  昆蟲(chóng)學(xué)報(bào). 2012(05)
[2]抑制劑存在下不同種群煙粉虱乙酰膽堿酯酶殘余活性頻率分布及其與抗藥性的關(guān)系[J]. 何玉仙,趙建偉,黃建,翁啟勇,梁智生.  昆蟲(chóng)學(xué)報(bào). 2009(12)
[3]Invasive mechanism and management strategy of Bemisia tabaci（Gennadius） biotype B:Progress report of 973 Program on invasive alien species in China[J]. WAN FangHao1,ZHANG GuiFen1,LIU ShuSheng2,LUO Chen3,CHU Dong4,ZHANG YouJun4,ZANG LianSheng2,JIU Min2,Lü ZhiChuang1,CUI XuHong1,ZHANG LiPing4,ZHANG Fan3,ZHANG QingWen5,LIU WanXue1,LIANG Pei5,LEI ZhongRen1 ＆ ZHANG YongJun1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Acadeny of Agriculture Sciences,Beijing 100094,China;2 Institute of Applied Entomology,Zhejiang University,Hangzhou 310029,China;3 Institute of Plant and Environment Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China;4 Institute of Vegetables and Flowers,Chinese Acadeny of Agricullare Sciences,Beijing 100081,China;5 Department of Entomology,China Agriculture University,Beijing 100094,China.  Science in China（Series C:Life Sciences）. 2009(01)
[4]B型煙粉虱對(duì)三種寄主植物及其揮發(fā)物的行為反應(yīng)[J]. 曹鳳勤,劉萬(wàn)學(xué),范中南,萬(wàn)方浩,程立生.  昆蟲(chóng)學(xué)報(bào). 2008(08)
[5]B型煙粉虱成蟲(chóng)對(duì)五種寄主植物的取食和產(chǎn)卵行為[J]. 林克劍,吳孔明,張永軍,郭予元.  植物保護(hù)學(xué)報(bào). 2008(03)
[6]溫室白粉虱的性行為[J]. 李祖蔭,烏里希.馬施維茨.  昆蟲(chóng)學(xué)報(bào). 1985(02)



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