禾谷鐮孢菌Fusarium graminearum是近年來引起大豆根腐病的重要致病菌之一,嚴(yán)重影響大豆產(chǎn)量和品質(zhì)。當(dāng)前,化學(xué)防治病害導(dǎo)致了諸如農(nóng)藥殘留、土壤水體污染、病菌抗藥性及生態(tài)環(huán)境破壞等一系列問題。選育和利用抗病品質(zhì)仍然是防治大豆病害的最經(jīng)濟、有效和環(huán)境友好的途徑。然而,關(guān)于大豆和禾谷鐮孢菌的互作機制,特別是大豆對禾谷鐮孢菌的抗性機制尚不清楚,有待深入研究。本論文以課題組前期抗性篩選獲得禾谷鐮孢菌中抗大豆品種南豆12和感病九月黃為材料,采用下胚軸刺傷接種法接種禾谷鐮孢菌,于接種后不同時間點檢測大豆下胚軸種抗氧化物酶（SOD、POX,GR,CAT和POD）活性、丙二醛(MDA,H₂O₂含量、活性氧ROS產(chǎn)生情況、大豆異黃酮含量變化,以及抗病相關(guān)基因表達(dá)情況,以期從生理生化和分子水平解析大豆響應(yīng)禾谷鐮孢菌侵染的機制。主要研究結(jié)果如下:禾谷鐮孢菌侵染后,三種抗氧化物酶活性和MDA含量均發(fā)生變化,其中抗病品種南豆12中CAT和SOD活性較感病品種九月黃高,且MDA含量較九月黃高,而在九月黃中POD活性在病菌侵染早期高于南豆12,后期又降低。受F... 

【文章來源】：四川農(nóng)業(yè)大學(xué)四川省 211工程院校

【文章頁數(shù)】：53 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
Abstract
Abbreviation
1. Introduction
    1.1 The occurrence and harm of soybean root rot
    1.2 Pathogen population of soybean root rot is complex
    1.3 Fusarium root rot occurs widely in soybean production
    1.4 F. graminearum as the important pathogens of soybean root rot
    1.5 Effective management of Fusarium related diseases
    1.6 Resistance mechanism of soybean to Fusarium graminearum
2. Purpose and Significance of this study
3. Materials and Methods
    3.1 Fungal culture
    3.2 Soybean germination and fungal inoculation
    3.3 Detection of antioxidant enzymes activities
    3.4 Detection of ROS (Reactive oxygen species)
    3.5 Examination of necrotic cell death
    3.6 HPLC Analysis of Isoflavones
    3.7 Analysis of defense-related gene expression
    3.8 Statistical analysis
4. Results
    4.1 Activity of Antioxidant enzyme was induced by F. graminearum but differed in two cultivars
    4.2 ROS were produced differently in two soybean cultivars after F. graminearum infection
    4.3 Cell death was induced differently in two soybean cultivars after F. graminearum infection
    4.4 Four isoflavones were significantly induced by F. graminearum but differed in quantity and change dynamics
    4.5 Expression of defense-related genes were induced by F. graminearum
5. Discussion
6. Conclusion
Reference
Dedication
Acknowledgement
Publications list


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期刊論文
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