植物寄生線蟲系一類嚴(yán)重威脅農(nóng)業(yè)生產(chǎn)的重要的植物病原生物。線蟲本身或和土壤中其他微生物的協(xié)同作用可以侵染和危害植物的根、莖、葉、果實(shí)和種子等。長(zhǎng)針類和毛刺類線蟲均系植物根的外寄生線蟲,是刺嘴綱（Enoplea）內(nèi)具有植物寄生能力的典型類群,長(zhǎng)針類和毛刺類線蟲可寄生危害許多的植物類群,更重要的是其中的長(zhǎng)針線蟲屬、擬長(zhǎng)針線蟲屬、劍線蟲屬、毛刺線蟲屬和擬毛刺線蟲屬內(nèi)的一些種類可以作為一些重要植物病毒的傳播介體。這些屬中的新種或者潛在的重要種類的鑒定及描述對(duì)農(nóng)業(yè)上植物檢疫等措施,抑制其傳播有重要的意義。本研究的目的是通過形態(tài)學(xué)和分子生物學(xué)的方法和技術(shù)提供準(zhǔn)確的有關(guān)長(zhǎng)針類和毛刺類線蟲的鑒定及檢測(cè)方法,以及中國(guó)發(fā)生的長(zhǎng)針類和毛刺類線蟲種群與國(guó)際上報(bào)道的有關(guān)種群的系統(tǒng)發(fā)育關(guān)系。本研究通過對(duì)來自國(guó)內(nèi)浙江、湖北、山東、安徽、海南、山西、云南、重慶和北京等9個(gè)�。ㄊ校﹥�(nèi),涉及公園、森林、果園等生物棲息地的植物根圍共采集528份土壤樣品（其中包括進(jìn)境植物根圍截獲的樣品）。通過改進(jìn)的過篩法進(jìn)行線蟲的分離,線蟲種的形態(tài)學(xué)鑒定系通過來源于多岐檢索表的分類特征和已報(bào)道種群原始描述的形態(tài)學(xué)特征及形態(tài)測(cè)量值比較,同時(shí)所有... 

【文章來源】：浙江大學(xué)浙江省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：174 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
Acknowledgement
摘要
Abstract
Chapter 1. Literature Review
    1.1 Nematode in Biodiversity Perspective
    1.2 Nematode in Ecological Web
    1.3 Mechanism of Nematode Interaction
    1.4 Advancement of Nematode Taxonomy
    1.5 Overview of Family Longidoridae and Trichodoridae
    1.6 Longidorids and Trichodorids Ancestry of Plant Parasitism
        1.6.1 The Family Longidoridae
        1.6.2 The Family Trichodoridae
            1.6.2.1 Taxonomy and Systematics
            1.6.2.2 General Morphology
            1.6.2.3 Biology, Feeding and Life Cycle
            1.6.2.4 Trichodorids - Tobravirus Association
Chapter 2. Materials and Methods
    2.1 Sampling Sites
    2.2 Soil collection and Nematode Extraction
    2.3 Morphological Examination
    2.4 Molecular Analysis
        2.4.1 DNA Extraction
        2.4.2 PCR and sequencing
    2.5 Phylogenetic Analysis
Chapter 3. Morphological and Molecular characterizations of Longidorid and Trichodoridnematodes occurring in 9 Provinces of China
    3.1 Nematode species identified during the study
    3.2 Morphological characterization of Longidorids occurring in China
        3.2.1 Family Longidoridae Thorne, 1935 (Meyl,1961)
    3.3 Morphological Identification of Trichodorids nematodes in China
        3.3.1 Family Trichodoridae (Thorne, 1953)Clark, 1961
Chapter 4. Molecular analysis and Phylogenetic relationships of Longidorid and Trichodoridnematodes in this study
    4.1 Phylogenetic relationships of Longidorus and Xiphinema based on D2-D3 segment of 28Sregion
    4.2 Phylogenetic relationships of Longidorus and Xiphinema based on ITS 1 region
    4.3 Phylogenetic relationships of Longidorus and Xiphinema based on partial 18S region
    4.4 Phylogenetic relationships of X. brevicolle intraspecies based on mitochondrial COI region
    4.5 Phylogenetic relationships of Trichodorus and Paratrichodorus based on D2-D3 segment of28 S region
    4.6 Phylogenetic relationships of Trichodorus and Paratrichodorus based on ITS1 region
    4.7 Phylogenetic relationships of Trichodorus and Paratrichodorus based on partial 18S region
Chapter 5. Discussions and Conclusion
REFERENCES


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期刊論文
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