發(fā)布時(shí)間：2021-09-18 11:29

　　蜂王漿是工蜂咽下腺等腺體分泌的漿狀物質(zhì),主要用以飼喂蜜蜂小幼蟲和蜂王。為了提高蜂王漿產(chǎn)量,我國(guó)從意大利蜜蜂（意蜂）中成功培育出蜂王漿高產(chǎn)蜜蜂（漿蜂）,蜂王漿產(chǎn)量至少提高了10倍�；诘鞍踪|(zhì)組學(xué)技術(shù),咽下腺發(fā)育和功能的分子機(jī)制研究已廣泛開展,為蜂王漿高產(chǎn)機(jī)制的解析提供了科學(xué)依據(jù)。但受過(guò)去蛋白質(zhì)組學(xué)發(fā)展技術(shù)的制約,只有少部分蛋白質(zhì)得以鑒定和分析,因此,隨著以質(zhì)譜技術(shù)為主蛋白組學(xué)技術(shù)的快速發(fā)展,需要借助新技術(shù)對(duì)蜜蜂蛋白質(zhì)譜進(jìn)行深入系統(tǒng)研究。本研究比較了漿蜂和意蜂的新出房蜂、哺育蜂和采集蜂的咽下腺蛋白質(zhì)組,在去除咽下腺里435個(gè)分泌蛋白后,即蜂王漿蛋白,在漿蜂和意蜂的咽下腺中共鑒定了5,751個(gè)蛋白,約占蜜蜂總蛋白組覆蓋率的38%,顯著提高咽下腺蛋白質(zhì)組覆蓋深度。功能富集分析結(jié)果表明,不同日齡咽下腺鑒定的蛋白和上調(diào)表達(dá)蛋白在漿蜂和意蜂中所鑒定蛋白和差異表達(dá)主要參與蛋白質(zhì)合成和糖類代謝支撐了腺體在不同階段的基本生物學(xué)需求,如幼蜂時(shí)期的腺體發(fā)育,哺育和采集蜂時(shí)期的蜂王漿蛋白質(zhì)和花蜜轉(zhuǎn)化酶分泌等的需求。然而漿蜂和意蜂咽下腺在不同發(fā)育階段都具有發(fā)育階段特異的蛋白表達(dá)譜幫助腺體實(shí)現(xiàn)各個(gè)階段的生物學(xué)功能。... 

【文章來(lái)源】：中國(guó)農(nóng)業(yè)科學(xué)院北京市

【文章頁(yè)數(shù)】：138 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER1 INTRODUCTION
    1. The honey bees
        1.1 The beekeeping industry
        1.2 Chinese beekeeping industry
        1.3 Economic importance of the honeybee
            1.3.1 Honey
            1.3.2 Beeswax
            1.3.3 Royal Jelly
            1.3.4 Pollen
            1.3.5 Other products
            1.3.6 Pollination
        1.4 The honeybee colony and the cast dependent role of worker bees
            1.4.1 The honeybee queen
            1.4.2 The honeybee drones
            1.4.3 The honeybee workers
        1.5 Anatomy of the honeybee
            1.5.1 The Hypopharyngeal Glands (HGs)
CHAPTER2 QUALITATIVE AND QUANTITATIVE PROTEOME COMPARISON OF HYPOPHARYNGEALGLANDS (HGs) OF ITALIAN HONEYBEES (APIS MELLIFERA) AT DIFFERENT AGES
    2.1 SUMMARY
    2.2 INTRODUCTION
    2.3 OBJECTIVE
    2.4 MATERIALS AND METHOD
        2.4.1 Reagents
        2.4.2 Sampling hypopharyngeal glands of ITBs
        2.4.3 Protein extraction and digestion
        2.4.4 LC?MS/MS analysis
        2.4.5 Identification and abundance level quantitation of proteins
        2.4.6 Bioinformatics analysis
    2.5 RESULTS
        2.5.1 Qualitative proteome comparison of HG development in ITBs
        2.5.2 Quantitative proteome comparison of HG development in ITB
    2.6 DISCUSSION
        2.6.1 Proper development and basic functions of the HGs requires shared proteome
        2.6.2 Age-dependent development and functions of the HGs requires age-specific proteins
    2.7 CONCLUSION
CHAPTER3 QUALITATIVE AND QUANTITATIVE PROTEOME COMPARISON OF HYPOPHARYNGEAL GLANDS (HGs) OF HIGH ROYAL JELLY PRODUCING HONEYBEES (RJBs) (APIS MELLIFERALIGUSTICA) AT DIFFERENT AGES
    3.1 SUMMARY
    3.2 INTRODUCTION
    3.3 OBJECTIVE
    3.4 MATERIALS and METHODS
        3.4.1 Reagents
        3.4.2 Sampling hypopharyngeal glands of RJBs
    3.5 RESULTS
        3.5.1 Qualitative proteome comparison of HG development in RJBs
        3.5.2 Quantitative proteome comparison of HG development in RJBs
    3.6 Discussion
        3.6.1 Proper development and basic functions of the HGs requires shared proteome
        3.6.2 Age-dependent development and functions of the HGs requires age-specific Proteins
    3.7 CONCLUSION
CHAPTER4 PROTEOME COMPARISON OF HYPOPHARYNGEAL GLANDS (HGS) BETWEEN TWO STOCKS OF HONEYBEES REVEALS DEEP INSIGHT INTO MOLECULAR UNDERLINGS OF HGDEVELOPMENT, FUNCTIONALITY AND DIFFERENCE
    4.1 SUMMARY
    4.2 INTRODUCTION
    4.3 OBJECTIVE
    4.4 MATERIALS AND METHOD
        4.4.1 Reagents
        4.4.2 Sampling hypopharyngeal lands from ITBs and RJBs
    4.5 RESULT
        4.5.1 Proteome comparison of HG between NEBs from ITBs and RJBs
        4.5.2 Proteome comparison of HG between NBs from ITBs and RJBs
        4.5.3 Proteome comparison of HGs between FBs from ITBs and RJBs
    4.6 DISCUSSION
        4.6.1 Proper development and basic functions of the NEBs HGs requires shared proteome
        4.6.2 The NEBs development from ITBs and RJBs adapt divergent HG proteome
        4.6.3 Proper development and basic functions of the NBs HGs requires shared Proteome
        4.6.4 The NBs development from ITBs and RJBs adapt divergent HG proteome
        4.6.5 Proper development and basic functions of the FBs HGs requires shared proteome
        4.6.6 The FBs development from ITBs and RJBs adapt divergent HG proteome
    4.7 CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
LIST OF TABLES
AUTHOR’S BIOGRAPHY


【參考文獻(xiàn)】：
期刊論文
[1]Effect of honey on bacterial translocation and intestinal morphology in obstructive jaundice[J]. Cem Gencay,Kemal Kismet,Bulent Kilicoglu,Serap Erel,Mehmet Ali Akkus,Sibel Serin Kilicoglu,Sabahattin Muratoglu,Asli Elif Sunay,Esra Erdemli.  World Journal of Gastroenterology. 2008(21)



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