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水稻根系相關(guān)內(nèi)生細(xì)菌的分離鑒定及生防潛能研究

發(fā)布時(shí)間:2021-06-26 03:13
  水稻(Oryza sativa L.)是世界上最重要的糧食作物之一。水稻在生長(zhǎng)的過程中受到各種病原菌的影響,其中植物病原真菌可使水稻產(chǎn)生多種重要的病害,這些水稻疾病的爆發(fā)導(dǎo)致嚴(yán)重的經(jīng)濟(jì)損失。目前防治這些水稻病害的主要途徑是使用化學(xué)農(nóng)藥,然而化學(xué)農(nóng)藥長(zhǎng)期、大量不合理的使用導(dǎo)致其產(chǎn)生的抗藥性,抗藥性菌株的出現(xiàn)已成為病害頻繁爆發(fā)的最主要因素。隨著經(jīng)濟(jì)社會(huì)的快速發(fā)展,人們對(duì)農(nóng)產(chǎn)品質(zhì)量安全和生態(tài)環(huán)境的要求越來越高,這迫使我國(guó)必須盡快研究和開發(fā)出替代化學(xué)農(nóng)藥的綠色安全高效生物防治制劑以有效地防治水稻病害。生防微生物及其代謝產(chǎn)物具有防效高、環(huán)境友好且對(duì)人畜無毒等優(yōu)點(diǎn),是一類理想的可替代化學(xué)農(nóng)藥的產(chǎn)品。根系內(nèi)的內(nèi)生細(xì)菌及其代謝產(chǎn)物具有抑制植物病原菌的活性,從而增強(qiáng)植物對(duì)病原菌的抵抗力。許多內(nèi)生細(xì)菌由于對(duì)植物病原菌具有良好的抑制活性,已經(jīng)被開發(fā)成生物防治劑在田間廣泛使用。然而,水稻根系內(nèi)生細(xì)菌對(duì)植物病原真菌的研究卻鮮有報(bào)道。本研究選取水稻田間不同品種的健康植株,對(duì)水稻根系的內(nèi)生細(xì)菌進(jìn)行了分離鑒定,測(cè)定了內(nèi)生細(xì)菌對(duì)幾種重要水稻真菌病原物的抑制活性,并對(duì)內(nèi)生細(xì)菌生防機(jī)制進(jìn)行了初步探究,具體的研究結(jié)果如下:1... 

【文章來源】:中國(guó)農(nóng)業(yè)科學(xué)院北京市

【文章頁(yè)數(shù)】:131 頁(yè)

【學(xué)位級(jí)別】:博士

【文章目錄】:
摘要
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 GENERAL INTRODUCTION
    1.2 REVIEW LITERATURE
        1.2.1 Introduction of bacterial endophytes
        1.2.2 Diversity of bacterial endophytes
        1.2.3 The Lifestyles of the bacterial endophytes
        1.2.4 Colonization behavior of bacterial endophytes
        1.2.5 Colonization mechanism of the bacterial endophytes in the host plant
        1.2.6 Function and role of plant bacterial endophytes
        1.2.7 Evolution of bacterial endophytes in plant by symbioses
        1.2.8 Biocontrol mechanism of endophytic bacterial
        1.2.9 Antagonistic mechanisms of bacterial endophytes by using biocontrol agents
        1.2.10 Secondary metabolite producing ability of bacterial endophytes
        1.2.11 Biotic and abiotic stresses tolerance abilities of bacterial endophytes
        1.2.12 Bacterial endophytes suppressing plant phytopathogens by anti-biotics and anti-biosis
        1.2.13 Induced systemic resistance(ISR)by bacterial endophytes against plant phytopathogens
CHAPTER 2 ISOLATION AND CHARACTERIZATION OF ROOT-ASSOCIATED BACTERIAL ENDOPHYTES AND THEIR BIOCONTROL POTENTIAL AGAINST MAJOR FUNGAL PHYTOPATHOGENS OF RICE(ORYZA SATIVA L.)
    2.1 INTRODUCTION
    2.2 MATERIAL AND METHODS
        2.2.1 Plant material and samples locations
        2.2.2 Surface sterilization of rice root tissues
        2.2.3 Isolation of root-associated bacterial endophytes
        2.2.4 Biochemical and physiological characterization of root-associated bacterial endophytes
        2.2.5 Genomic DNA extraction of root-associated bacterial endophytes
        2.2.6 PCR amplification and classification of root-associated bacterial endophytes
        2.2.7 Phylogenetic analysis of root-associated bacterial endophytes based on16s r RNA gene sequencings
        2.2.8 Rice Fungal Phytopathogens and Culture Condition
        2.2.9 Molecular detection of antibiotic related genes based on PCR amplification of root-associated bacterial endophytes
        2.2.10 Statistical Analysis
    2.3 RESULTS
        2.3.1 Isolation and identification of root-associated bacterial endophytes
        2.3.2 Molecular classification and phylogenetic association of root-associated bacterial endophytes
        2.3.3 Biochemical and physiological characterization of root-associated bacterial endophytes
        2.3.4 In-vitro antagonistic activities of root-associated bacterial endophytes
        2.3.5 Molecular detection of antibiotic related genes based on PCR amplification of root-associated bacterial endophytes
    2.4 DISCUSSION
    2.5 CONCLUSION
CHAPTER3 MAJOR FINDINGS AND FUTURE PROSPECTIVES
    3.1 MAJOR FINDINGS
    3.2 FUTURE PROSPECTIVES
REFERENCES
APPENDIX
LIST OF PUBLICATIONS
ACKNOWLEDGEMENTS
AUTHORS RESUME


【參考文獻(xiàn)】:
期刊論文
[1]Gram-Positive Bacteria Associated with Rice in China and Their Antagonists Against the Pathogens of Sheath Blight and Bakanae Disease in Rice[J]. LUO Jin-yan1, XIE Guan-lin1, LI Bin1, LUO Yuan-chan1, ZHAO Li-han1, WANG Xiao 1, LIU Bo2, LI Wen1 (1 State Key Laboratory of Rice Biology, Zhejiang University,Hangzhou 310029, China; 2 Center of Biotechnology, Fujian Academy of Agricultural Science, Fuzhou 350003, China).  Rice Science. 2005(03)



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