【摘要】：本研究建立以離子液體作為萃取溶液,聚丙烯中空纖維膜作為支載體,三相微萃取尿液中的目標(biāo)物質(zhì),以期建立阿片類毒品的高效液相色譜檢測方法(IL-HF-LPME-HPLC)。實驗以嗎啡、曲馬多、可待因、美沙酮四種阿片類毒品為研究對象,優(yōu)化出最佳的HF-LPME的萃取模式和條件為:萃取劑為3-辛基-3-甲基咪唑六氟磷酸離子液體加15%三辛基氧化膦;接收相均為p H為1,加入Na Cl為2 mol/L,攪拌速度為1 000 r/min,萃取時間為60 min。通過本優(yōu)化的方法檢測4種阿片類物質(zhì),獲得了較高的富集倍數(shù)(98~178倍)和較低的檢測限(10~50 ng/m L),相對標(biāo)準(zhǔn)偏差(RSD)均為小于10%。研究表明,中空纖維膜起到了很好的隔離復(fù)雜基質(zhì)中的大分子干擾作用,具有十分良好的富集效果。
[Abstract]:In order to establish a high performance liquid chromatography (IL-HF-LPME-HPLC) method for the determination of opioid drugs, a three-phase microextraction of target substances from urine with ionic liquid as extraction solution and polypropylene hollow fiber membrane as branch carrier was established in order to establish a high performance liquid chromatography (HPLC) method for the determination of opioid drugs. Four opioid drugs, morphine, tramadol, codeine and methadone, were studied. The optimum extraction mode and conditions of HF-LPME were optimized as follows: the extractant was 3-octyl-3-methylimidazolium hexafluorophosphate ionic liquid plus 15% trioctyl phosphine oxide, the receiving phase was pH 1, the stirring speed of Na Cl was 1 000 r 路min, and the extraction time was 60 min.. Four opioids were detected by this optimized method, and the higher enrichment multiple (98 鈮，

本文編號：2514844