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不同真空抗凝管保存對(duì)血醇含量穩(wěn)定性的影響

發(fā)布時(shí)間:2018-10-21 16:56
【摘要】:目前對(duì)酒駕取血存在血儲(chǔ)容器種類繁多、缺乏規(guī)范的現(xiàn)象,為進(jìn)一步優(yōu)選血儲(chǔ)容器,最大限度保證血醇含量測(cè)定結(jié)果的穩(wěn)定,為血樣存儲(chǔ)最適容器的選擇和交管部門(mén)制定相應(yīng)規(guī)范、標(biāo)準(zhǔn)提供基礎(chǔ)的數(shù)據(jù)。本實(shí)驗(yàn)選取5種常見(jiàn)的真空抗凝管,在恒溫冰箱(4~8℃)下存儲(chǔ)靜脈鮮血,采用GC法測(cè)定血液0、3 d、7 d、14 d、21 d和28 d的乙醇動(dòng)態(tài)含量。結(jié)果顯示:血儲(chǔ)容器為枸櫞酸鈉(1:4)黑色帽管在3 d后、氟化鈉-草酸鉀灰色帽管在7 d后血醇含量呈不斷顯著下降的趨勢(shì)(p0.05);EDTA-k2紫色帽管、枸櫞酸鈉(1:9)藍(lán)色帽管、肝素綠色帽管在21 d內(nèi)酒精含量穩(wěn)定性較好,之后顯著下降(p0.05)。5種采血管以EDTA-k2紫色帽管、枸櫞酸鈉(1:9)藍(lán)色帽管、肝素綠色帽管在21 d內(nèi)為較佳血儲(chǔ)容器,其余2種抗凝管不宜作為血醇檢測(cè)的血儲(chǔ)容器。
[Abstract]:At present, there are many kinds of blood storage containers for drinking driving blood, which is lack of standard. In order to further optimize the selection of blood storage containers and to ensure the stability of the determination results of blood alcohol content to the maximum extent, To provide basic data for the selection of optimal vessel for blood sample storage and for traffic control department. In this experiment, five kinds of common vacuum anticoagulant tubes were used to store venous blood in a constant temperature refrigerator (48 鈩,

本文編號(hào):2285762

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