【摘要】：目的研究重慶地區(qū)漢族人群KIR基因及基因型多態(tài)性特點(diǎn)。方法采集316名重慶地區(qū)漢族無償獻(xiàn)血者的外周血樣,應(yīng)用多重PCR-SSP方法作KIR基因分型;根據(jù)等位基因頻率網(wǎng)絡(luò)數(shù)據(jù)庫(AFND)中的ID號(hào)命名檢測(cè)到的基因型及單體型組成,以Spss17.0軟件對(duì)人群間KIR基因頻率作卡方檢驗(yàn)。結(jié)果 316名重慶地區(qū)漢族獻(xiàn)血者個(gè)體均含有KIR基因3DL2、3DL3;2DL4、2DL1、3DP1、2DP1、2DL3、3DL1較為常見,頻率分別為94.1%、92.0%、92.0%、90.3%、87.4%、76.8%;頻率較低(30%)的基因有2DL5、2DS1、3DS1、2DS5、2DL2、2DS2和2DS3。共發(fā)現(xiàn)24種KIR基因型,其中以ID號(hào)為1、2和8的基因型最為常見,頻率分別為54.4%(172/316)、15.2%(48/316)和6.3%(20/316),還有1種基因型無法在AFND中查出其基因型。重慶地區(qū)漢族獻(xiàn)血者攜帶的KIR各基因中分別有8個(gè)和7個(gè)基因的頻率與南非圣族人和高加索人明顯不同(P0.05),有5個(gè)與烏市維族人明顯不同(P0.05),僅有1個(gè)與拉薩藏族有明顯不同(P0.05),而與四川漢族、云南漢族、浙江漢族、江蘇漢族和韓國(guó)人均無明顯差異(P0.05)。結(jié)論重慶地區(qū)漢族人群的KIR基因分析既含豐富的多態(tài)性,又具有自身的特點(diǎn),與四川、云南等中國(guó)南方漢族人群一致。
[Abstract]:Objective to study the polymorphism of KIR gene and genotype in Chongqing Han population. Methods Peripheral blood samples were collected from 316 non-remunerated blood donors of Han nationality in Chongqing, and the genotypes and haplotypes were detected according to the ID number of the allele frequency network database (AFND), and the multiple PCR-SSP method was used for KIR genotyping. The frequency of KIR gene was detected by chi-square with Spss17.0 software. Results the 3DL2DL3DL3DL3DL4DL4DL4DL2DL2DL2DL3DL3DL2DL2DL3DL1 gene 3DL2DL2DL2DL3DL1 was found in 316 blood donors of Han nationality in Chongqing. The frequencies of 3DL2DL1DL3DL3DL2D2D2D2D2DL2D2D2DL2D2DS2DS2DS2DS2DS2DS2and 2DS3D3D2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2D2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2DS2@@ A total of 24 genotypes of KIR were found, of which ID number 1G 2 and genotype 8 were the most common, with frequencies of 54.4% (17.2 / 316) and 6.3% (20 / 316), respectively. One genotype could not be detected in AFND. The frequencies of 8 and 7 of the KIR genes carried by Han blood donors in Chongqing area were significantly different from those of the South African saints and Caucasians (P0.05), and 5 of them were significantly different from those of Uighurs in Wushi (P0.05), and only one of them had the same frequency with Lhasa Tibetans. The difference was significant (P0.05), but it was different from that of the Han nationality in Sichuan. There was no significant difference between Han people in Yunnan, Zhejiang, Jiangsu and Korea (P0.05). Conclusion the KIR gene analysis of Han population in Chongqing area is not only rich in polymorphism, but also has its own characteristics, which is consistent with the Han population in southern China, such as Sichuan and Yunnan.
【作者單位】： 重慶市血液中心;中國(guó)醫(yī)學(xué)科學(xué)院輸血研究所;
【基金】：重慶市科委自然基金資助項(xiàng)目(CSTC,2010BB5339)
【分類號(hào)】：R394
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本文編號(hào)：2144386