【摘要】：藥敏試驗(yàn)是臨床選擇敏感抗生素的精確檢測(cè)工作,其成敗在于質(zhì)量控制的可靠性和最低抑菌濃度(MIC)的精確性、前瞻性.本文結(jié)合質(zhì)控試驗(yàn)和藥敏試驗(yàn)對(duì)梯度平板法進(jìn)行驗(yàn)證,梯度平板法所測(cè)得的MIC與微量肉湯稀釋法和瓊脂稀釋法測(cè)得的MIC結(jié)果一致,且因?yàn)榫�(xiàn)性設(shè)置的藥物濃度,MIC更加精確,能推算抗菌藥物的原始濃度,更好地保證質(zhì)控試驗(yàn)的可靠性.同時(shí),按照美國(guó)臨床實(shí)驗(yàn)室標(biāo)準(zhǔn)委員會(huì)或歐盟藥敏試驗(yàn)委員會(huì)的敏感(S),中介(I)以及抗藥(R)范圍標(biāo)準(zhǔn)設(shè)置0-S,S-R,R-8R這樣3個(gè)線(xiàn)性梯度,首先判斷菌株敏感性范圍,然后精確測(cè)定細(xì)菌群落的MIC值,并能有效分離出抗性菌株,預(yù)警該抗生素使用后可能出現(xiàn)的抗藥性,進(jìn)而指導(dǎo)臨床抗菌藥物的選擇和應(yīng)用.梯度平板的主要困難在于手工制備的繁瑣和不穩(wěn)定性,如果自動(dòng)化生產(chǎn)即用型梯度平板,將便利臨床藥敏試驗(yàn),改進(jìn)抗藥性篩選技術(shù).
[Abstract]:Drug sensitivity test is an accurate test for clinical selection of sensitive antibiotics. Its success or failure lies in the reliability of quality control and the accuracy and prospective of minimum inhibitory concentration (MIC). In this paper, the quality control test and drug sensitivity test were combined to verify the gradient plate method. The MIC determined by gradient plate method was consistent with that of the micro broth dilution method and the Agar dilution method, and because the linear setting of drug concentration and MIC was more accurate, the results obtained by the gradient plate method were in good agreement with those obtained by the micro broth dilution method and the Agar dilution method. The original concentration of antimicrobial agents can be calculated and the reliability of quality control test can be better guaranteed. At the same time, according to the sensitivity (S), intermediary (I) and drug resistance (R) range standards of the American Committee for Clinical Laboratory Standards or the European Commission for Drug sensitivity testing, three linear gradients such as 0-S S-RN R-8R were set up to determine the sensitivity range of the strain. Then the MIC value of bacterial community was determined accurately, and the resistant strain could be effectively isolated, and the resistance of the antibiotic could be warned, and the selection and application of clinical antimicrobial agents could be guided. The main difficulty of gradient plate is the complexity and instability of manual preparation. If the gradient plate is produced automatically, it will facilitate the clinical drug sensitivity test and improve the screening technique of drug resistance.
【作者單位】： 山東省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所山東省動(dòng)物疫病防治與繁育重點(diǎn)實(shí)驗(yàn)室;山東大學(xué)生命科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(批準(zhǔn)號(hào):81271886,31672609) 山東省農(nóng)業(yè)重大應(yīng)用技術(shù)創(chuàng)新項(xiàng)目(批準(zhǔn)號(hào):SDAIT-11-09) 山東省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)創(chuàng)新工程(批準(zhǔn)號(hào):CXGC2016A10) 山東省自然科學(xué)培養(yǎng)基金(批準(zhǔn)號(hào):ZR2014CP021)資助
【分類(lèi)號(hào)】：R446.5
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本文編號(hào)：2124663