發(fā)布時間：2018-06-14 22:06

  本文選題：降鈣素原 + 時間分辨熒光法　； 參考：《軍事醫(yī)學》2017年08期

【摘要】：目的建立一種基于時間分辨熒光納米顆粒的磁分離均相免疫分析方法并用于檢測降鈣素原(PCT)重組抗原。方法以PCT單克隆抗體偶聯(lián)銪離子熒光微球為檢測抗體,另一株PCT單克隆抗體偶聯(lián)生物素作為固相抗體,建立一種通過表面修飾有鏈霉親和素的磁珠分離銪離子熒光微球-檢測抗體-降鈣素原-固相抗體-生物素復合物的均相免疫分析方法。用該法檢測PCT重組抗原,通過Sigma Plot分析該法的最低檢測限。結果 PCT重組抗原的標準曲線匹配二次函數(shù),函數(shù)方程為Y=19170.12+75493.74X-26.00X2(r=0.9986),PCT重組抗原檢測靈敏度為0.04 ng/ml。結論銪離子熒光微球可作為標記物應用于磁分離均相免疫分析方法,并在PCT重組抗原檢測中獲得較高的檢測靈敏度。
[Abstract]:Objective to establish a time resolved fluorescent nanoparticles based magnetic separation homogeneous immunoassay method for the detection of procalcitonin PCT recombinant antigen. Methods PCT monoclonal antibody coupled with europium ion fluorescent microspheres was used as detection antibody and another strain of PCT monoclonal antibody coupled with biotin as solid phase antibody. A homogeneous immunoassay method was developed for the separation of europium ion fluorescent microspheres by magnetic beads modified with streptavidin on the surface-detection antibody-procalcitonin-solid phase antibody-biotin complex. PCT recombinant antigen was detected by this method, and the minimum detection limit was analyzed by Sigma blot. Results the standard curve matching quadratic function of PCT recombinant antigen was used. The function equation was: YT19170.12 75493.74X-26.00X2RX 0.9986. The detection sensitivity of PCT recombinant antigen was 0.04 ng / ml. Conclusion europium ion fluorescent microspheres can be used as markers for magnetic separation homogenous immunoassay, and high sensitivity can be obtained in the detection of PCT recombinant antigen.
【作者單位】： 安徽醫(yī)科大學;軍事醫(yī)學科學院微生物流行病研究所病原微生物生物安全國家重點實驗室;
【分類號】：R446.6

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本文編號：2019109