本文選題：雌激素 + 疼痛　； 參考：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年07期

【摘要】：目的·通過(guò)建立去勢(shì)小鼠模型并給予外源性雌激素補(bǔ)充治療,探索雌激素調(diào)控去勢(shì)小鼠疼痛的炎癥機(jī)制。方法·24只12周齡C57BL/6J雌性小鼠隨機(jī)分為3組:假手術(shù)組(S組),手術(shù)+安慰劑對(duì)照組(P組),手術(shù)+雌激素替代治療組(E組)。術(shù)后1周給予雌激素替代治療,為期4周。分別在術(shù)前1日、術(shù)后3日及術(shù)后1、2、3、4、5周檢測(cè)小鼠基礎(chǔ)痛閾,包括機(jī)械痛閾縮足閾值(PWT)和熱板痛閾縮足潛伏期(PWL);術(shù)后3日及5周檢測(cè)小鼠血清炎癥因子水平,包括IL-1β、IL-6、TNF-α;術(shù)后5周取小鼠脊髓腰膨大,Western blotting分析p38表達(dá)含量。結(jié)果·去勢(shì)小鼠術(shù)后基礎(chǔ)痛閾均較S組低,PWT、PWL分別在術(shù)后第1周、術(shù)后第3日起與S組差異有統(tǒng)計(jì)學(xué)意義(P0.01)。術(shù)后第3日,E組、P組炎癥因子水平均較S組高,差異具有統(tǒng)計(jì)學(xué)意義(P0.01)。術(shù)后第5周,P組p38表達(dá)高于S組,差異具有統(tǒng)計(jì)學(xué)意義(P0.01)。給予雌激素補(bǔ)充治療后,術(shù)后第4、5周,E組PWT、PWL均略有提高,與P組差異具有統(tǒng)計(jì)學(xué)意義(P0.01)。術(shù)后第5周E組炎癥因子表達(dá)降低,與P組差異具有統(tǒng)計(jì)學(xué)意義(P0.01),但也仍然顯著高于S組(P0.01)。術(shù)后第5周E組p38表達(dá)含量降低但仍高于S組,與P組差異具有統(tǒng)計(jì)學(xué)意義(P0.01),而與S組比較差異已無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論·雌激素可能通過(guò)p38 MAPK通路降低炎癥因子水平,發(fā)揮對(duì)去勢(shì)小鼠疼痛的調(diào)控作用。
[Abstract]:Objective to explore the inflammatory mechanism of estrogen regulating pain in ovariectomized mice by establishing ovariectomized mice model and giving exogenous estrogen supplementation therapy. Methods Twenty-four 12-week-old female C57BL/6J mice were randomly divided into three groups: sham operation group (group S), surgical placebo control group (group P) and estrogen replacement therapy group (group E). Estrogen replacement therapy was given 1 week after operation for 4 weeks. The basic pain threshold (PWTs) and the latent period of hot plate pain threshold (PWTs) were measured at 1 day before operation, 3 days after operation and 5 weeks after operation, and the levels of serum inflammatory factors in mice were measured at 3 and 5 weeks after operation. The expression of p38 was detected by Western blotting at 5 weeks after operation. Results the basic pain threshold of castrated mice was significantly lower than that of group S at 1 week and 3 days after operation respectively. There was significant difference between group S and group S (P 0.01). The level of inflammatory factors in group E was higher than that in group S on the 3rd day after operation, and the difference was statistically significant (P 0.01). The expression of p38 in P group was higher than that in S group at 5 weeks after operation, and the difference was statistically significant (P 0.01). After estrogen supplementation therapy, PWTG PWL in group E increased slightly at the 4th week after operation, which was significantly higher than that in group P (P 0.01). At the 5th week after operation, the expression of inflammatory factors in group E was significantly lower than that in group P (P 0.01), but still significantly higher than that in group S (P 0.01). At the 5th week after operation, the expression of p38 in group E was decreased but still higher than that in group S, and there was a significant difference between group E and group P (P 0.01), but there was no significant difference between group E and group S (P 0.05). Conclusion estrogen may reduce the level of inflammatory cytokines through p38 MAPK pathway and play a role in regulating pain in ovariectomized mice.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院麻醉科;
【基金】：國(guó)家自然科學(xué)基金(81400910)~~
【分類號(hào)】：R402

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