本文選題：銅綠假單胞菌 + 泛耐藥�。� 參考：《中華醫(yī)院感染學(xué)雜志》2017年19期

【摘要】：目的調(diào)查一組泛耐藥的銅綠假單胞菌獲得性耐藥元件基因的攜帶情況及菌株間的親緣關(guān)系。方法收集2015年1-12月醫(yī)院住院患者痰液標(biāo)本分離到的20株泛耐藥的銅綠假單胞菌,采用K-B法測(cè)定9種抗菌藥物的敏感性,采用聚合酶鏈反應(yīng)(PCR)及序列分析的方法分析35種β-內(nèi)酰胺類(lèi)獲得性耐藥基因與膜孔蛋白o(hù)prD2基因、21種氨基糖苷類(lèi)獲得性耐藥基因和9種可移動(dòng)遺傳元件遺傳標(biāo)記。陽(yáng)性耐藥基因測(cè)序后直接作BLAST比對(duì),對(duì)57種獲得性耐藥元件基因與膜孔蛋白o(hù)prD2基因檢測(cè)結(jié)果作樣本聚類(lèi)分析(UPGMA法)。結(jié)果 20株銅綠假單胞菌為泛耐藥菌。20株菌有19株分別檢出1~3種β-內(nèi)酰胺酶基因,僅1株未檢出β-內(nèi)酰胺酶基因且膜孔蛋白o(hù)prD2基因缺失。20株菌均檢出氨基糖苷類(lèi)藥物獲得性耐藥基因和可移動(dòng)遺傳元件遺傳標(biāo)記基因。樣本聚類(lèi)分析提示20株菌有聚集性且可分成A、B群。其中A群中有4個(gè)克隆播散,B群中有2個(gè)克隆播散。結(jié)論 20株泛耐藥銅綠假單胞菌同時(shí)攜帶了β-內(nèi)酰胺類(lèi)藥物獲得性耐藥基因、氨基糖苷類(lèi)藥物獲得性耐藥基因和可移動(dòng)遺傳元件遺傳標(biāo)記,是對(duì)β-內(nèi)酰胺類(lèi)和氨基糖苷類(lèi)產(chǎn)生耐藥的重要原因。本組菌檢出的6個(gè)克隆高度疑似醫(yī)院感染,同一克隆菌株攜帶相同基因。
[Abstract]:Objective to investigate the gene carriers of acquired drug resistance elements of Pseudomonas aeruginosa and the relationship between them. Methods from January to December 2015, 20 strains of pan-resistant Pseudomonas aeruginosa were isolated from sputum samples of hospital inpatients. The sensitivity of 9 antimicrobial agents was determined by K-B method. Polymerase chain reaction (PCR) and sequence analysis were used to analyze 35 尾 -lactam acquired drug resistance genes and 21 aminoglycoside acquired resistance genes and 9 transportable genetic element genetic markers. The positive drug resistance genes were compared with BLAST directly after sequencing. The results of 57 acquired drug resistance element genes and membrane porin oprD2 gene were analyzed by cluster analysis with UPGMA method. Results among the 20 strains of Pseudomonas aeruginosa, 19 strains were pan-resistant. Only one strain did not detect 尾 -lactamase gene and the membrane-porin oprD2 gene was absent. All the 20 strains detected aminoglycoside acquired drug resistance gene and transportable genetic element genetic marker gene. Cluster analysis showed that 20 strains of bacteria were clustered and could be divided into Agna B group. There are 4 clones in group A and 2 in group B. Conclusion 20 strains of pan-resistant Pseudomonas aeruginosa carry 尾 -lactam acquired drug resistance genes, aminoglycoside acquired drug resistance genes and transportable genetic element genetic markers. It is an important cause of resistance to 尾-lactams and aminoglycosides. The 6 clones were highly suspected of nosocomial infection, and the same cloned strains carried the same gene.
【作者單位】： 湖北醫(yī)藥學(xué)院附屬襄陽(yáng)市第一人民醫(yī)院檢驗(yàn)科;寧波市第一醫(yī)院檢驗(yàn)科;
【基金】：浙江省中醫(yī)藥基金資助項(xiàng)目(2011ZB126)
【分類(lèi)號(hào)】：R446.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉春明;韋柳華;朱勝波;唐石伏;孫一帆;馬興璇;;耐藥銅綠假單胞菌獲得性耐藥基因與可移動(dòng)遺傳元件檢測(cè)的指標(biāo)聚類(lèi)分析[J];中華醫(yī)院感染學(xué)雜志;2012年22期

，

本文編號(hào)：1824071