本文選題：血小板 + 無菌試驗(yàn)�。� 參考：《中國(guó)輸血雜志》2017年07期

【摘要】：目的分析增加厭氧培養(yǎng)是否降低血小板細(xì)菌污染引起的臨床輸血不良反應(yīng)。方法采用全自動(dòng)微生物培養(yǎng)檢測(cè)系統(tǒng)對(duì)9 758份單采血小板進(jìn)行需氧、厭氧培養(yǎng),調(diào)查單采血小板厭氧培養(yǎng)的細(xì)菌檢出率、檢出時(shí)間、檢出細(xì)菌的種類以及臨床輸注后的反應(yīng)。結(jié)果需氧培養(yǎng)和厭氧培養(yǎng)的陽性率分別為0.06%(6/9 758)和0.16%(16/9 758),增加厭氧培養(yǎng)使細(xì)菌污染檢出率從0.06%增加到0.16%,提高了2.6倍。其中有10份血小板樣本僅在厭氧培養(yǎng)中檢出的細(xì)菌,檢出的細(xì)菌以瘡皰丙酸桿菌等厭氧菌為主,檢出時(shí)間為(96.8±18.21)h,檢出時(shí)均已臨床輸注,未出現(xiàn)不良反應(yīng)。結(jié)論增加厭氧培養(yǎng)可提高血小板細(xì)菌污染的檢出率,但是本研究中厭氧瓶未在血小板發(fā)放使用前出現(xiàn)陽性信號(hào)。在目前我國(guó)逐步開展血小板細(xì)菌檢測(cè)的情況下,可參考北美洲和中國(guó)香港的模式首先開展需氧培養(yǎng)以減少血小板細(xì)菌污染引起的臨床輸血不良反應(yīng)。
[Abstract]:Objective to analyze whether increasing anaerobic culture can reduce the adverse reaction of blood transfusion caused by bacterial contamination of platelets. Methods A total of 9 758 platelets were cultured for aerobic and anaerobic culture with an automatic microbiological culture system. The detection rate, detection time, types of bacteria and the reaction after transfusion were investigated. Results the positive rates of aerobic culture and anaerobic culture were 0.06 / 9.758 and 0.16 / 9.758, respectively. Increasing anaerobic culture increased the detection rate of bacterial contamination from 0.06% to 0.16%, which increased 2.6 times. Among them, 10 platelet samples were only detected in anaerobic culture. The main bacteria detected were Propionibacterium bullosa and other anaerobic bacteria, and the detection time was 96.8 鹵18.21 h. All of them were infused clinically and no adverse reactions were found at the time of detection. Conclusion the increase of anaerobic culture can increase the detection rate of platelet bacterial contamination, but the anaerobic flask did not show positive signals before platelet release. Under the condition that platelet bacteria are being detected step by step in our country, the aerobic culture should be carried out first in order to reduce the adverse reaction of blood transfusion caused by bacterial contamination of platelets, according to the model of North America and Hong Kong, China.
【作者單位】： 浙江省血液中心;
【分類號(hào)】：R457.1

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