本文選題：埃博拉病毒 + 磁球�。� 參考：《分析科學學報》2017年05期

【摘要】：用免疫磁球捕獲埃博拉病毒糖蛋白,與生物素化抗體形成免疫夾心復合物,然后鏈霉親和素標記辣根過氧化物酶(SA-HRP)催化3,3',5,5'-四甲基聯(lián)苯胺(TMB)進行顯色,通過370 nm處吸光度對病毒糖蛋白進行定量。對免疫磁球進行了免疫熒光表征,通過對照實驗驗證方法的可靠性,并對檢測條件進行了優(yōu)化。結(jié)果表明,吸光度與病毒糖蛋白濃度在1.0~25.0 ng/m L內(nèi)呈線性關系,檢出限達到0.18 ng/m L。該方法重現(xiàn)性較好,特異性好,抗干擾能力強,可實現(xiàn)復雜樣品中埃博拉病毒的檢測。
[Abstract]:The Ebola virus glycoprotein was captured with the immunomagnetic ball, and the immunofluorescence complex was formed with the biotinylated antibody. Then the streptomycin labeled horseradish peroxidase (SA-HRP) catalyzed 3,3', 5,5'- four methylbiphenyl amine (TMB) to color and quantified the virus sugar protein through the absorbance at 370 nm. The immunomagnetic sphere was immunofluorescent table. The results showed that the absorbance and the concentration of virus glycoprotein in 1.0~25.0 ng/m L were linear, and the detection limit reached 0.18 ng/m L.. The results showed that the method had good reproducibility, good specificity and strong anti-interference energy, and the detection of Ebola virus in complex samples could be realized.

【作者單位】： 生物醫(yī)學分析化學教育部重點實驗室武漢大學化學與分子科學學院;
【基金】：國家自然科學基金(No.21475099)
【分類號】：O657.3;R446.6
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本文編號：1798590