本文選題：固相化檢測 + 抗體篩查��； 參考：《中國輸血雜志》2017年07期

【摘要】：目的對隨機獻血者及臨床輸血患者紅細胞、血小板、淋巴細胞與中性粒細胞的抗體進行固相化聯(lián)合篩查體系。方法將單層混合多人份血細胞分別固定在U型微孔板中,制備出固相化抗體篩查體系。然后對隨機獻血樣本2 150例及臨床輸血患者輸血后樣本440例血漿中是否含有相應(yīng)抗體進行檢測,并分析對對輸血后效果的影響。結(jié)果隨機獻血者樣本中抗體篩出率為:紅細胞抗體53例(2.5%),血小板抗體22例(1%),淋巴細胞抗體13例(0.6%),中性粒細胞抗體55例(2.6%);臨床輸血患者輸血后樣本中抗體篩出率為:紅細胞抗體31例(7%),血小板抗體38例(8.6%),淋巴細胞抗體24例(5.5%),中性粒細胞抗體43例(7.5%),混合抗體(包括同時存在兩種或兩種以上抗體)115例(26.1%)。223次(22.8%)輸血后產(chǎn)生疑似不良反應(yīng),其中133例(約60%)檢出抗體陽性。結(jié)論血細胞抗體固相化篩查體系可應(yīng)用于臨床輸血前及輸血后抗體檢測。固相化檢測體系靈敏度高于試管法血清學(xué)檢測與流式細胞術(shù)�？贵w的存在對輸血效果存在一定的影響。
[Abstract]:Objective to screen the antibodies of erythrocytes, platelets, lymphocytes and neutrophils in random blood donors and clinical transfusion patients. Methods monolayer mixed human blood cells were immobilized in U-shaped microporous plates to prepare solid-phase antibody screening system. Then, 2 150 samples of random blood donors and 440 cases of blood samples after clinical blood transfusion were tested for the presence of corresponding antibodies, and the influence on the effect of blood transfusion was analyzed. Results the screening rate of antibodies in random blood donors was: red blood cell antibody 53 cases, platelet antibody 22 cases, lymphocyte antibody 13 cases, neutrophil antibody 55 cases and neutrophil antibody 55 cases respectively, and the antibody screening rate of clinical blood transfusion patients after blood transfusion was as follows: 22 cases of platelet antibody, 22 cases of platelet antibody, 13 cases of lymphocyte antibody and 0.6%, 55 cases of neutrophil antibody. There were 31 cases of RBC antibody, 38 cases of platelet antibody, 24 cases of lymphocytic antibody, 43 cases of neutrophil antibody and 7.5%, and 115 cases of mixed antibody (including two or more kinds of antibody). Among them 133 cases (about 60%) were positive for antibody. Conclusion Blood cell antibody solid phase screening system can be used to detect antibody before and after transfusion. The sensitivity of solid phase detection system was higher than that of test tube method and flow cytometry. The presence of antibodies has a certain effect on the effect of blood transfusion.
【作者單位】： 上海市血液中心輸血研究所;中國人民解放軍第四五五醫(yī)院檢驗科;
【基金】：上海市衛(wèi)生和計劃生育委員會青年項目(20124Y079) 上海市自然科學(xué)基金(11ZR1434000)
【分類號】：R457.1

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