本文選題：細(xì)胞凋亡 + PUMA-α蛋白　； 參考：《解放軍醫(yī)學(xué)雜志》2017年09期

【摘要】：目的探討p53上調(diào)凋亡調(diào)節(jié)因子(PUMA)-α蛋白在高糖刺激誘導(dǎo)的人腹膜間皮細(xì)胞(HPMC)凋亡及纖維化中的作用。方法以50mmol/L D型葡萄糖和甘露醇分別刺激HPMC 72h后,采用流式細(xì)胞儀檢測細(xì)胞凋亡率,Western blotting檢測凋亡及纖維化相關(guān)蛋白的表達(dá)。分別以Lenti-PUMA-α和sh RNA-PUMA-α轉(zhuǎn)染正常的HPMC和高糖刺激72h后的HPMC,采用流式細(xì)胞儀檢測凋亡細(xì)胞數(shù)目,Western blotting檢測凋亡及纖維化相關(guān)蛋白的表達(dá)。結(jié)果高糖刺激HPMC 72h后,流式細(xì)胞儀檢測結(jié)果顯示細(xì)胞凋亡率增加;Western blotting檢測結(jié)果顯示,促進(jìn)凋亡及纖維化的蛋白表達(dá)增加,而抑制凋亡及纖維化的蛋白表達(dá)減少。上調(diào)PUMA-α的表達(dá)可促進(jìn)HPMC的凋亡及纖維化;下調(diào)PUMA-α的表達(dá)可抑制HPMC的凋亡及纖維化(P0.05)。結(jié)論高糖刺激通過上調(diào)PUMA-α的表達(dá),促進(jìn)了HPMC的凋亡及纖維化。
[Abstract]:Objective to investigate the role of p53 up-regulation of apoptosis regulatory factor (PUMA- 偽) protein in hyperglycemic induced apoptosis and fibrosis of human peritoneal mesothelial cells (HPMCs).Methods after stimulation of HPMC with 50mmol/L D glucose and mannitol for 72 hours, apoptosis rate was detected by flow cytometry and the expression of fibrosis related protein was detected by Western blotting.Lenti-PUMA- 偽 and sh RNA-PUMA- 偽 were transfected into normal HPMC and HPMC stimulated by high glucose for 72 h, respectively. The number of apoptotic cells and the expression of fibrosis-related protein were detected by flow cytometry and Western blotting.Results after stimulation of HPMC with high glucose for 72 hours, the results of flow cytometry showed that the rate of apoptosis was increased. Western blotting analysis showed that the expression of protein that promoted apoptosis and fibrosis increased, but the protein expression of inhibiting apoptosis and fibrosis decreased.Upregulating the expression of PUMA- 偽 could promote the apoptosis and fibrosis of HPMC, and down-regulating the expression of PUMA- 偽 could inhibit the apoptosis and fibrosis of HPMC.Conclusion High glucose stimulates apoptosis and fibrosis of HPMC by upregulating the expression of PUMA- 偽.
【作者單位】： 解放軍273醫(yī)院內(nèi)三科;解放軍69008部隊衛(wèi)生隊;解放軍273醫(yī)院內(nèi)一科;陜西省友誼醫(yī)院消化內(nèi)科;第四軍醫(yī)大學(xué)西京醫(yī)院腎臟內(nèi)科;
【分類號】：R459.5
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本文編號：1736144