本文選題：非連續(xù)密度梯度離心　切入點(diǎn)：梯度分離　出處：《中國實(shí)驗(yàn)血液學(xué)雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的:研究非連續(xù)密度梯度分離法分離紅細(xì)胞的影響因素,初步建立Percoll分離液在紅細(xì)胞分離中的最佳操作條件。方法:檢測Percoll與1.5 mol/L NaCl比例為9∶1、10∶1、11∶1、12∶1、13∶1滲透壓情況,選取等滲比例作為最佳母液(SIP);以不同密度細(xì)胞被充分離心沉降分離,較少存在紅細(xì)胞滯留、拖帶現(xiàn)象作為分離效果評判標(biāo)準(zhǔn),比較不同紅細(xì)胞懸液濃度、不同離心力、不同離心加減速度、不同溫度和不同離心時間的分離效果。結(jié)果:50%以下濃度紅細(xì)胞懸液分離效果優(yōu)于50%以上濃度紅細(xì)胞懸液,4 000×g優(yōu)于3 000和2 000×g的離心效果,2-8的離心加減速度可以獲得滿意的離心效果,4℃分層效果比室溫穩(wěn)定,20 min優(yōu)于15和10 min的分離效果。結(jié)論:Percoll與1.5mol/L NaCl為12∶1和11∶1的母液可作為等滲母液,優(yōu)先4℃離心條件離心,加減速度2-8,其離心效果會隨著紅細(xì)胞懸液濃度的降低、離心時間的延長、離心力的增加而獲得更好的分離效果
[Abstract]:Objective: to study the factors affecting the separation of red blood cells by discontinuous density gradient separation, and to establish the optimum operating conditions of Percoll separation solution in erythrocyte separation. Methods: the osmotic pressure of Percoll to 1.5 mol/L NaCl was 9: 1: 10: 1: 11: 11: 12: 1: 1: 1. The isosmotic ratio was chosen as the best mother liquid, the cells of different density were separated by full centrifugation and sedimentation, there was less red blood cell retention, the phenomenon of towing was taken as the criterion for evaluating the separation effect, and the different concentration of red blood cell suspension and different centrifugal force were compared. Different centrifugal speed, Results the separation effect of red blood cell suspensions below 50% was better than that of red blood cell suspensions of more than 50% concentration (4 000 脳 g) than that of 3 000 脳 g and 2 000 脳 g / 2 000 脳 g centrifuge suspensions. The results show that the separation effect at 4 鈩，

本文編號：1616716