本文關鍵詞： 醒腦靜注射液 膿毒癥 心肌酶 線粒體呼吸鏈復合物　出處：《中國臨床藥理學雜志》2017年07期 　論文類型：期刊論文

【摘要】：目的研究醒腦靜注射液對膿毒癥大鼠心肌酶和線粒體呼吸鏈復合物的影響。方法按照體重將SD大鼠隨機分為3組:正常組10只,腹腔注射與脂多糖(LPS)等量的0.9%NaCl;其余SD大鼠腹腔注射10 mg·kg~(-1)脂多糖(LPS),建立膿毒癥模型。模型組與實驗組均30只,實驗組經(jīng)胃管注入醒腦靜注射液6.4 mL·kg~(-1)。于給藥后6,24,48 h,用試劑盒法檢測不同時點各組的心肌酶和呼吸鏈復合物的活性,包括NADH-輔酶Q還原酶(complexⅠ)、琥珀酸-輔酶Q還原酶(complexⅡ)、輔酶Q-細胞色素C還原酶(complexⅢ)、細胞色素C-氧化還原酶(complexⅣ)。結(jié)果在24 h,實驗組與模型組的血清肌酸激酶分別為(1333.20±140.87),(1813.50±243.72)U·L~(-1);這2組的肌酸激酶同工酶分別為(748.15±119.02),(1167.92±145.17)U·L~(-1)。實驗組的肌酸激酶、肌酸激酶同工酶水平均顯著低于模型組,差異均有統(tǒng)計學意義(P0.01)。在24 h,實驗組和模型組的心肌線粒體complexⅠ活性分別為(2.83±0.15),(1.84±0.18)u·mg~(-1),這2組的complexⅢ活性分別為(0.91±0.13),(0.68±0.16)u·mg~(-1),這2組的complexⅣ分別為(19.43±1.37),(15.20±1.79)u·mg~(-1),實驗組心肌線粒體complexⅠ、Ⅲ、Ⅳ活性均顯著高于模型組差異均有統(tǒng)計學意義(P0.05)。結(jié)論醒腦靜注射液可顯著降低膿毒癥大鼠心肌酶水平,提高膿毒癥大鼠心肌線粒體complexⅠ、Ⅲ及Ⅳ活性。
[Abstract]:Objective to study the effect of Xingnaojing injection on myocardial enzyme and mitochondrial respiratory chain complex in septic rats. Methods SD rats were randomly divided into 3 groups according to their body weight: 10 normal rats. The sepsis model was established by intraperitoneal injection of 0.9% NaCl1 to lipopolysaccharide (LPS) and intraperitoneal injection of 10 mg 路kg ~ (-1) lipopolysaccharide (LPS-1) into the other SD rats. Xingnaojing injection (6.4 mL 路kg ~ (-1)) was injected into the stomach tube of the experimental group. The activity of myocardial enzyme and respiratory chain complex at different time points were detected by kit method at 6 ~ 24m ~ 48 h after administration. These include NADH- coenzyme Q reductase complex 鈪，

本文編號：1511552