本文關(guān)鍵詞：四川自貢地區(qū)健康人群中艾伯特埃希菌的分離鑒定　出處：《中國人獸共患病學(xué)報》2017年06期 　論文類型：期刊論文

  更多相關(guān)文章： 艾伯特埃希菌 健康人群 eae MLST PFGE

【摘要】：目的了解長期從事雞鴨等家禽養(yǎng)殖及屠宰人員等健康人群艾伯特埃希菌的攜帶情況。方法收集家禽養(yǎng)殖、屠宰人群及其他健康人糞便,經(jīng)EC肉湯增菌后PCR檢測eae基因,陽性樣品接種麥康凱瓊脂,挑選不發(fā)酵乳糖菌落,通過16SrDNA序列分析和多位點序列分型(MLST)對菌株進行鑒定。分離株進行緊密粘附素亞型和cdtB亞型分析,并通過脈沖場凝膠電泳(PFGE)分析與動物來源艾伯特埃希菌菌株間的相關(guān)性。結(jié)果從189份從事雞鴨宰殺人員的糞便中,分離到2株艾伯特埃希菌,從58份其他健康人群糞便中分離到1株菌,而138份家禽養(yǎng)殖場人員糞便中未分離到菌株。3株菌株的緊密粘附素亞型分別為sigma,iota 2,nu,cdtB亞型均為Ⅱ/Ⅲ/Ⅴ亞型。PFGE顯示三株菌之間的帶型相似性小于80%,并且與雞鴨等來源的艾伯特埃希菌帶型不同。結(jié)論從事雞鴨屠宰等健康人員中存在一定程度的艾伯特埃希菌攜帶率,但其與家禽攜帶艾伯特埃希菌的關(guān)系,仍需進一步研究。
[Abstract]:Objective to investigate the carrying status of Ebert-Escherichia coli in poultry breeding and slaughtermen for a long time. Methods the feces of poultry, slaughtered people and other healthy people were collected. The eae gene was detected by PCR after inoculation with EC broth. The positive samples were inoculated with wheat Kang Kai Agar and the unfermented lactose colony was selected. The strains were identified by 16s rDNA sequence analysis and multilocus sequence typing. The close adhesion subtypes and cdtB subtypes of the isolates were analyzed. The correlation between Ebert-Escherichia coli strain and animal origin was analyzed by pulsed field gel electrophoresis (PFGE). Results two strains were isolated from 189 feces of chicken and duck slaughtermen. One strain was isolated from the feces of 58 other healthy people, while the close adhesion subtype of strain .3 was sigma-iota 2 in 138 feces of poultry farm workers. The results showed that the similarity of banding type among the three strains was less than 80%. The subtypes of nuchus cdtB were all 鈪，

本文編號：1438170