本文關(guān)鍵詞：低頻電磁場(chǎng)對(duì)毛發(fā)生長(zhǎng)及相關(guān)因子表達(dá)影響的實(shí)驗(yàn)研究　出處：《南方醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 低頻電磁場(chǎng) 組織形態(tài) 基因表達(dá) 毛發(fā)生長(zhǎng)

【摘要】：目的觀察低頻電磁場(chǎng)在毛發(fā)生長(zhǎng)周期小鼠模型中對(duì)毛發(fā)生長(zhǎng)及KGF、TGF-β1、PDGF-BB及FGFR2表達(dá)的影響。方法將120只毛發(fā)自然生長(zhǎng)模型C57BL/6小鼠隨機(jī)分成磁場(chǎng)組和對(duì)照組,每組60只。其中,磁場(chǎng)組小鼠選擇低頻電磁場(chǎng)(50 Hz、5mT)暴露,30min/d,連續(xù)干預(yù)14天;對(duì)照組不進(jìn)行磁場(chǎng)處理,但飼養(yǎng)條件與磁場(chǎng)組一致。實(shí)驗(yàn)過程中,分別在磁場(chǎng)處理后的第3d、7d、14d留取小鼠背部皮膚組織。肉眼觀察毛發(fā)生長(zhǎng)差異;HE染色觀察毛發(fā)及表皮組織形態(tài);熒光定量PCR法檢測(cè)KGF、TGF-β1、PDGF-BB 及 FGFR2 表達(dá);Western Blot 檢測(cè) KGF、TGF-β1、PDGF-BB 及 FGFR2蛋白表達(dá)。結(jié)果1、肉眼觀察,3d時(shí)可見兩組小鼠背部毛發(fā)呈黑色,色澤鮮亮、濃密;7d時(shí)毛發(fā)逐漸脫落,變稀疏;至14d時(shí),兩組毛發(fā)幾乎脫落完,脫毛處皮膚變粉紅。第3d、7d時(shí),磁場(chǎng)組和對(duì)照組中小鼠的毛發(fā)生長(zhǎng)情況無明顯差異。2、HE染色觀察:與對(duì)照組相比,磁場(chǎng)干預(yù)后第3d、7d,小鼠毛發(fā)長(zhǎng)度增加(P0.05),同時(shí)小鼠表皮厚度也增厚(P0.05);當(dāng)磁場(chǎng)干預(yù)后第14天時(shí),毛發(fā)生長(zhǎng)進(jìn)入退行期,與對(duì)照組相比較,其表皮厚度較厚。在干預(yù)過程中,對(duì)照組和磁場(chǎng)組的生長(zhǎng)周期無明顯差別。3、PCR檢測(cè)結(jié)果:在磁場(chǎng)組和對(duì)照組中,組內(nèi)第3d、7d、14d時(shí)間點(diǎn)中的KGF的表達(dá)逐漸降低,但變化差異無統(tǒng)計(jì)學(xué)意義(P0.05);與對(duì)照組相比,同時(shí)間點(diǎn)的磁場(chǎng)組KGFmRNA的表達(dá)增加,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。在對(duì)照組中,第3d、7d、14d時(shí)間點(diǎn)中的TGF-β1的表達(dá)逐漸增加,但變化差異無統(tǒng)計(jì)學(xué)意義(P0.05),而磁場(chǎng)組是先增加后降低,差異也無統(tǒng)計(jì)學(xué)意義(P0.05);與對(duì)照組相比,3d、7d磁場(chǎng)組TGF-β1的表達(dá)均增加,14d時(shí)磁場(chǎng)組低于對(duì)照組,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。在磁場(chǎng)組和對(duì)照組中,組內(nèi)第3d、7d、14d時(shí)間點(diǎn)中的PDGF-BB的表達(dá)是先增加后減少,但變化差異無統(tǒng)計(jì)學(xué)意義(P0.05);3d、7d兩組的表達(dá)均高于14d(P0.05),與對(duì)照組相比,同時(shí)間點(diǎn)的磁場(chǎng)組PDGF-BB的表達(dá)下降,差異不具有統(tǒng)計(jì)學(xué)意義(P0.05)。在磁場(chǎng)組和對(duì)照組中,組內(nèi)第3d、7d、14d時(shí)間點(diǎn)中的FGFR2的表達(dá)逐漸降低,但變化差異無統(tǒng)計(jì)學(xué)意義(P0.05);與對(duì)照組相比,同時(shí)間點(diǎn)的磁場(chǎng)組FGFR2的表達(dá)增加,差異不具有統(tǒng)計(jì)學(xué)意義(P0.05)。4、Western-blot檢測(cè)結(jié)果:每個(gè)時(shí)間點(diǎn)KGF蛋白的表達(dá)磁場(chǎng)組較對(duì)照組均稍有增加,對(duì)照組KGF蛋白表達(dá)隨時(shí)間變化不大,磁場(chǎng)組在7d時(shí)KGF蛋白表達(dá)較3d、14d的均高。在每個(gè)時(shí)間點(diǎn)TGF-β1的蛋白表達(dá)均是磁場(chǎng)組高于對(duì)照組;而兩組的TGF-β1的蛋白表達(dá)隨時(shí)間變化不大。在每個(gè)時(shí)間點(diǎn),PDGF-BB的表達(dá)均是磁場(chǎng)組高于對(duì)照組;對(duì)照組在3d和7d時(shí)表達(dá)變化不大,但到14d時(shí)表達(dá)減小,而磁場(chǎng)組在7d時(shí)表達(dá)最多。FGFR2在3d、7d時(shí)的蛋白表達(dá)均是磁場(chǎng)組高于對(duì)照組,14d時(shí)兩組表達(dá)差異不明顯。結(jié)論低頻電磁場(chǎng)可促進(jìn)生長(zhǎng)周期中小鼠毛發(fā)的生長(zhǎng);在該過程中,低頻電磁場(chǎng)促進(jìn)KGF、TGF-β1、PDGF-BB及FGFR2的表達(dá)。
[Abstract]:Objective to observe the effect of low frequency electromagnetic field on hair growth and KGF- 尾 1 in mouse hair growth cycle. Methods 120 C57BL / 6 mice were randomly divided into magnetic field group and control group. The mice in the magnetic field group were exposed to low frequency electromagnetic field (50 Hz) for 30 min / d for 14 days. The control group was not treated with magnetic field, but the feeding condition was the same as that in the magnetic field group. During the experiment, the skin tissue of the back of the mice was taken on the 3rd day, 7 days and 14 days after the magnetic field treatment, and the difference of hair growth was observed with naked eyes. The morphology of hair and epidermis was observed by HE staining. The expression of PDGF-BB and FGFR2 was detected by fluorescence quantitative PCR. The expression of PDGF-BB and FGFR2 protein was detected by Western Blot. On the 3rd day, the hair on the back of the two groups was black, bright and dense. At 7 days, the hair gradually fell off and became sparse. At day 14, the hair of the two groups was almost lost, and the skin became pink at the point of depilation. On the 3rd day, there was no significant difference in hair growth between the magnetic field group and the control group. Observation of HE staining: compared with the control group, the length of hair and the thickness of epidermis of mice were increased on the 3rd day and 7th day after the treatment of magnetic field, and the thickness of epidermis was also increased. At the 14th day after the magnetic field intervention, the hair growth entered into the retreat period, compared with the control group, the thickness of the epidermis was thicker. In the intervention process, the growth cycle of the control group and the magnetic field group was not significantly different. 3. The results of PCR detection showed that the expression of KGF in the magnetic field group and the control group decreased gradually at the 14th day after the 3rd day, but there was no significant difference between the two groups (P 0.05). Compared with the control group, the expression of KGFmRNA increased in the magnetic field group at the same time point, and the difference was statistically significant (P 0.05). The expression of TGF- 尾 1 increased gradually at 14d, but there was no significant difference in the change of TGF- 尾 1 expression, while in the magnetic field group, the expression of TGF- 尾 1 increased first and then decreased, and the difference was not statistically significant. Compared with the control group, the expression of TGF- 尾 _ 1 in the magnetic field group was significantly higher than that in the control group on the 3rd day and 7th day, and the expression of TGF- 尾 _ 1 in the magnetic field group was lower than that in the control group at the 14th day, and the difference was not statistically significant (P 0.05). The expression of PDGF-BB was increased first and then decreased at the 14th day after the 3rd day, but there was no significant difference between the two groups (P 0.05). The expression of PDGF-BB in the magnetic field group at the same time point was lower than that in the control group. The difference was not statistically significant (P 0.05). In the magnetic field group and the control group, the expression of FGFR2 decreased gradually at the 14th day after the 3rd day. But the difference was not statistically significant (P 0.05). Compared with the control group, the expression of FGFR2 in the magnetic field group at the same time point was increased, but the difference was not statistically significant (P 0.05). Western-blot results: the expression of KGF protein in the magnetic field group was slightly higher than that in the control group at each time point, but the expression of KGF protein in the control group did not change with time. The expression of KGF protein in the magnetic field group was higher than that in the control group on the 7th day, and the expression of TGF- 尾 1 protein in the magnetic field group was higher than that in the control group at each time point. However, the expression of TGF- 尾 1 protein in the two groups did not change with time. The expression of PDGF-BB in the magnetic field group was higher than that in the control group at each time point. The expression of FGFR2 in the magnetic field group was significantly higher than that in the control group on the 3rd day and 7th day in the magnetic field group, but the expression of FGFR2 in the magnetic field group was higher than that in the control group at the 3rd and 7th day, but at the 14th day, the expression of the highest expression of FGFR2 in the magnetic field group was higher. Conclusion the low frequency electromagnetic field can promote the growth of hair in the growth cycle of mice. In this process, the low frequency electromagnetic field promoted the expression of PDGF-BB and FGFR2 in KGF- 尾 1.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R454.1

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