【摘要】：傳統(tǒng)射頻生物傳感方案中,傳感器件只能檢測(cè)干燥樣本。對(duì)細(xì)胞等具有生物活性的生物大分子進(jìn)行烘干處理后,生物活性降低,其電特性發(fā)生改變,從而影響最終檢測(cè)結(jié)果。為了在微波頻段靈敏檢測(cè)出生物分子溶液或液體的介電特性,本文提出基于對(duì)稱超材料元件作為諧振結(jié)構(gòu)的射頻生物傳感器。首先,介紹了基于超材料結(jié)構(gòu)的射頻生物傳感器的研究背景。其次,闡述諧振型生物傳感器的工作原理、諧振型超材料傳輸線設(shè)計(jì)方案及諧振結(jié)構(gòu)和生物溶液結(jié)合過(guò)程。詳細(xì)描述有效介質(zhì)、半波長(zhǎng)諧振器、左手介質(zhì)和超材料傳輸線四個(gè)方面的生物傳感器設(shè)計(jì)的理論基礎(chǔ)。通過(guò)開(kāi)環(huán)諧振器(split-ring resonator,SRR)或互補(bǔ)開(kāi)環(huán)諧振器(complementary split-ring resonator,CSRR)的純諧振方法或混合方法耦合可得到多種不同類型的傳輸線。再次,提出了基于開(kāi)環(huán)諧振器(nested split-ring resonator,nSRR)結(jié)構(gòu)的生物傳感器設(shè)計(jì)方案。仿真驗(yàn)證了被檢測(cè)溶液的介電常數(shù)、厚度及覆蓋位置對(duì)生物傳感器的影響。最后,闡明了新型生物傳感器的制備和測(cè)量校準(zhǔn)過(guò)程。生物傳感器裸片諧振頻率的測(cè)試結(jié)果與相應(yīng)的仿真結(jié)果相符,從而驗(yàn)證了仿真結(jié)果的正確性。開(kāi)展了聚二甲基硅氧烷(Polydimethysiloxane,PDMS)液體樣本測(cè)試實(shí)驗(yàn),并與相應(yīng)的仿真結(jié)果進(jìn)行了比較。綜上所述,本文利用阻抗匹配方法,設(shè)計(jì)出基于對(duì)稱超材料元件作為諧振結(jié)構(gòu)的射頻生物傳感器,該生物傳感器可用于免標(biāo)簽的生物分子溶液快速檢測(cè)。生物傳感器裸片的實(shí)驗(yàn)測(cè)試結(jié)果與仿真結(jié)果符合較好,驗(yàn)證了理論以及設(shè)計(jì)方案的正確性。通過(guò)PDMS液體樣本測(cè)試實(shí)驗(yàn),初步證明了生物傳感器可以通過(guò)測(cè)試添加液體前后諧振頻率的變化,推測(cè)被測(cè)生物分子液體或溶液的介電特性。
[Abstract]:In traditional RF biosensors, only dry samples can be detected by sensors. After drying the biological macromolecules, such as cells, the biological activity decreased and its electrical properties changed, thus affecting the final detection results. In order to detect the dielectric properties of biomolecular solution or liquid sensitively in microwave frequency band, a novel RF biosensor based on symmetric supermaterial as resonant structure is proposed in this paper. Firstly, the research background of RF biosensor based on supermaterial structure is introduced. Secondly, the working principle of resonant biosensor, the design scheme of resonant supermaterial transmission line and the process of combining resonant structure with biological solution are described. The theoretical basis of biosensor design in effective medium, half-wavelength resonator, left-handed medium and supermaterial transmission line is described in detail. Different types of transmission lines can be obtained by coupling the pure resonant method or the hybrid method of open loop resonator (split-ring resonator,SRR) or complementary open loop resonator (complementary split-ring resonator,CSRR). Thirdly, a design scheme of biosensor based on open-loop resonator (nested split-ring resonator,nSRR) is proposed. The effects of dielectric constant, thickness and cover position on the biosensor are verified by simulation. Finally, the preparation and measurement calibration process of the new biosensor are expounded. The test results of the resonance frequency of the biosensor are in good agreement with the corresponding simulation results, which verify the correctness of the simulation results. The liquid samples of polydimethylsiloxane (Polydimethysiloxane,PDMS) were tested and compared with the corresponding simulation results. In summary, using impedance matching method, a novel RF biosensor based on symmetric supermaterial as resonant structure is designed. The biosensor can be used for fast detection of biomolecular solution without label. The experimental results are in good agreement with the simulation results, which verify the correctness of the theory and the design scheme. The experiment of PDMS liquid sample shows that the biosensor can predict the dielectric properties of the biomolecular liquid or solution by testing the change of resonance frequency before and after adding liquid.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TP212.3

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本文編號(hào)：2448144