本文選題：干細胞　切入點：生物光子　出處：《天津工業(yè)大學》2017年碩士論文

【摘要】：干細胞是具有自我更新和高度增殖能力的一類細胞,在再生醫(yī)學領(lǐng)域具有廣闊的應用前景。大量具有高活力、無損傷的干細胞是進行干細胞生物學研究和臨床應用的重要前提。因此,實時準確地獲取細胞的生理狀態(tài)信息具有重要的研究價值。針對現(xiàn)有干細胞狀態(tài)信息檢測手段的局限性和不足,本文基于光學檢測技術(shù)的無標記、非侵入等優(yōu)勢,結(jié)合生物光子輻射對細胞生理狀態(tài)靈敏的指示特性,設(shè)計并搭建了適用于活體干細胞的生物光子檢測系統(tǒng),旨在為進一步研究生物超弱光子輻射與干細胞生理狀態(tài)的關(guān)聯(lián)性提供可靠的實驗平臺。本文的主要研究內(nèi)容如下:(一)根據(jù)生物光子的輻射強度低和輻射過程非穩(wěn)態(tài)等特點,基于單光子計數(shù)測量原理,結(jié)合多通道時序控制技術(shù),提出了一種適用于活體干細胞的生物光子檢測系統(tǒng)的設(shè)計方案。(二)采用高壓氙燈作為照射光源,利用光纖及耦合透鏡設(shè)計系統(tǒng)傳輸光路,使用食品級不銹鋼設(shè)計樣品暗室,提高了系統(tǒng)的靈活性和避光性。采用高靈敏、低噪聲的光電倍增管和快速響應的多通道光子計數(shù)器,提高了系統(tǒng)的集成度和靈敏度,降低了系統(tǒng)的噪聲水平。(三)針對活體干細胞延遲發(fā)光檢測對時序控制的需求,提出了一種基于ARM+FPGA架構(gòu)的多通道時序控制器的設(shè)計方案,并進行了儀器的產(chǎn)品化設(shè)計。以ARM芯片STM32F103ZET6和FPGA芯片EP3C16Q240C8N為核心設(shè)計了多通道時序控制器的硬件電路,并基于此硬件平臺進行了 FPGA邏輯電路設(shè)計,ARM軟件設(shè)計,觸摸屏的界面設(shè)計以及上位機軟件設(shè)計,實現(xiàn)了 8通道并行輸出、時序精度為1μs,雙通道觸發(fā)響應和以太網(wǎng)遠程控制等功能。(四)搭建了以多通道時序控制器為控制核心的活體干細胞生物光子檢測系統(tǒng),并進行了干細胞檢測實驗。實驗結(jié)果顯示本文所研制的活體干細胞生物光子檢測系統(tǒng)具有檢測精度高(4.6×105/s·pW,at 600nm)、噪聲水平低(暗計數(shù)低于20光子/秒)、測量滯后時間短(約35ms)、擴展性強、應用靈活性高等特點,滿足活體細胞光誘導延遲發(fā)光的測量要求,可為進一步研究生物超弱光子輻射與干細胞生理狀態(tài)信息的關(guān)聯(lián)性提供穩(wěn)定可靠的測量工具。
[Abstract]:Stem cells are a kind of cells with self-renewal and high proliferative ability, which have a broad application prospect in regenerative medicine.A large number of stem cells with high activity and no damage are the important prerequisite for the biological research and clinical application of stem cells.Therefore, it has important research value to obtain the physiological state information of cells in real time and accurately.In view of the limitations and shortcomings of the existing methods for the detection of stem cell state information, based on the advantages of non-labeling and non-invasion of optical detection technology, this paper combines the biological photon radiation to indicate the physiological state of the cells.A biological photon detection system suitable for living stem cells was designed and built to provide a reliable experimental platform for the further study of the relationship between biological ultra-weak photon radiation and the physiological state of stem cells.The main contents of this paper are as follows: (1) according to the characteristics of low radiation intensity and unstable radiation process of biological photons and based on the principle of single photon counting measurement, combined with multi-channel timing control technology,A design scheme of biological photon detection system for living stem cells is presented.(2) High pressure xenon lamp is used as irradiation light source, optical transmission path is designed by optical fiber and coupling lens, and sample darkroom is designed by using food grade stainless steel, which improves the flexibility and light avoidance of the system.The high sensitivity low noise photomultiplier tube and the fast response multichannel photon counter are used to improve the integration and sensitivity of the system and reduce the noise level of the system.(3) in order to meet the demand of time-sequence control for delayed luminescence detection of living stem cells, a design scheme of multi-channel timing controller based on ARM FPGA architecture is proposed, and the production design of the instrument is carried out.The hardware circuit of multi-channel timing controller is designed with ARM chip STM32F103ZET6 and FPGA chip EP3C16Q240C8N as the core. Based on this hardware platform, the design of FPGA logic circuit and arm software, interface design of touch screen and upper computer software design are carried out.The parallel output of 8 channels is realized, the timing precision is 1 渭 s, the trigger response of two channels and the remote control of Ethernet are realized.(4) the biological photon detection system of living stem cells with multi-channel timing controller as the control core is built, and the stem cell detection experiment is carried out.The experimental results show that the living stem cell biological photon detection system developed in this paper has the characteristics of high detection accuracy (4.6 脳 10 ~ 5 / s pWN / s), low noise level (dark count is less than 20 photons / sec), short measurement lag time (about 35 Ms / s), high expansibility, high flexibility in application, etc.It can provide a stable and reliable tool for further study on the relationship between biological ultra-weak photon radiation and physiological state information of stem cells.
【學位授予單位】：天津工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R329.2;TP274

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