本文選題：DNA　切入點(diǎn)：聚硫堇　出處：《吉首大學(xué)學(xué)報(bào)(自然科學(xué)版)》2016年06期 　論文類(lèi)型：期刊論文

【摘要】：在玻碳電極(GCE)表面依次電聚合硫堇膜(PTh)、電沉積金-普魯士藍(lán)復(fù)合納米粒子(PB-Au)和金納米粒子(GN),利用GN比表面積大和生物相容性好的特性,進(jìn)而固定單鏈DNA(ssDNA),制備一種電流型DNA傳感器(GCE/PTh/PB-Au/GN/ssDNA).利用電化學(xué)交流阻抗技術(shù)(EIS)和循環(huán)伏安法(CV)對(duì)電極的修飾過(guò)程進(jìn)行表征,以亞甲基藍(lán)(MB)為雜交指示劑,利用微分脈沖伏安法(DPV)對(duì)DNA進(jìn)行檢測(cè).結(jié)果表明,所制備的DNA傳感器可以對(duì)DNA進(jìn)行靈敏檢測(cè),在1.0×10~(-14)~1.0×10~(-6) mg/L范圍內(nèi),DPV的峰電流與DNA質(zhì)量濃度呈良好線(xiàn)性關(guān)系,工作曲線(xiàn)斜率為-13.4μA/decade,相關(guān)系數(shù)為0.994,檢測(cè)下限為1.0×10~(-14) mg/L.所制備的傳感器靈敏準(zhǔn)確,不僅可用于基因檢測(cè),而且對(duì)重金屬離子及其他有機(jī)污染物的檢測(cè)也有重要研究?jī)r(jià)值.
[Abstract]:On the surface of glassy carbon electrode (GCE), the electropolymerized thionine film (PThn), electrodeposition of gold / Prussian blue composite nanoparticles (PB-Au) and gold nanoparticles (GNN) were sequentially used to make use of the characteristics of large GN surface area and good biocompatibility. A novel current mode DNA sensor, GCE-PTh-PB-Au-PB-Au-GNssDNA, was prepared by immobilization of single strand DNA. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the modification process of the electrode. Methylene blue blue MBs was used as the hybridization indicator, and the electrochemistry impedance spectroscopy (ECI) and cyclic voltammetry (CV) were used to characterize the modification process of the electrode. Differential pulse voltammetry (DPV) was used to detect DNA. The results show that the DNA sensor can be used to detect DNA sensitively. The peak current of DPV is linearly correlated with the mass concentration of DNA in the range of 1.0 脳 10 ~ (-14) ~ (-1) mg/L. The slope of the working curve is -13.4 渭 A / r decade, the correlation coefficient is 0.994, and the detection limit is 1.0 脳 10 ~ (-1) -14) mg / L. the sensor prepared is sensitive and accurate, which can be used not only for gene detection, but also for the detection of heavy metal ions and other organic pollutants.
【作者單位】： 遼寧師范大學(xué)化學(xué)化工學(xué)院功能材料化學(xué)研究所;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(21304041)
【分類(lèi)號(hào)】：O657.1;TP212.3

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