本文選題：脈沖染料激光 + 上皮間充質(zhì)轉(zhuǎn)化　； 參考：《激光雜志》2017年10期

【摘要】：目的:探討脈沖染料激光(pulsed dye laser,PDL)不同治療周期對兔耳創(chuàng)面上皮細胞向間質(zhì)細胞轉(zhuǎn)化的影響及該現(xiàn)象對瘢痕增生的作用。方法:新西蘭大耳白兔12只建立兔耳腹側(cè)增生性瘢痕模型,應(yīng)用595nm Vbeam脈沖染料激光分別采用一周、二周、三周的治療周期進行瘢痕的激光干預(yù),在治療前及治療2、4、6、8周測量瘢痕厚度,治療結(jié)束后取材行組織形態(tài)學觀察,免疫組織化學方法測定各組上皮間充質(zhì)轉(zhuǎn)化(Epithelial-mesenchymal Transformation,EMT)正相關(guān)蛋白及負相關(guān)蛋白的表達情況。結(jié)果:兔耳各組模型均有瘢痕增生,EMT表面標記物表達陽性;PDL治療結(jié)束后,一周治療組及二周治療組均表現(xiàn)出TGF-β1、Vim表達下調(diào),Ecad表達上調(diào),產(chǎn)生明顯的瘢痕抑制效應(yīng),與對照組相比有顯著差異;三周治療組與對照組、一周治療組和二周治療組相比無明顯差異。結(jié)論:PDL可能通過干預(yù)EMT的表達產(chǎn)生瘢痕抑制作用;治療周期為兩周較為適宜。
[Abstract]:Objective: to investigate the effect of pulsed dye laser (PDL) on the transformation from epithelial cells to interstitial cells in rabbit ear wound and the effect of this phenomenon on scar proliferation. Methods: the hypertrophic scar model was established in 12 New Zealand white rabbits. Laser intervention was performed with 595nm Vbeam pulsed dye laser for one week, two weeks and three weeks respectively. The thickness of scar was measured before treatment and 8 weeks after treatment. Histomorphology was performed after treatment. The expression of Epithelial-mesenchymal Transformation (EMTT) positive and negative correlation protein was detected by immunohistochemical method. Results: after the end of PDL treatment, the expression of EMT surface markers in each rabbit ear model showed a down-regulation of TGF- 尾 _ 1 Vim expression, which resulted in a marked scar inhibition effect in both the treatment group and the two-week treatment group, and the expression of TGF- 尾 _ 1 and Vim was down-regulated. There was no significant difference between the three-week treatment group and the control group, the one-week treatment group and the two-week treatment group. Conclusion the effect of chicatricial inhibition may be produced by intervention of the expression of EMT, and the treatment cycle is more suitable for two weeks.
【作者單位】： 遵義醫(yī)學院;重慶市涪陵中心醫(yī)院;
【基金】：重慶市衛(wèi)生計生委科研計劃項目(2014-2-148)
【分類號】：R622;TN249

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