廣州地區(qū)麗赤殼屬真菌的種類鑒定
發(fā)布時間：2018-11-19 08:23
【摘要】：麗赤殼屬(Calonectria)真菌是一類重要的植物病原菌,其分布范圍廣,可為害林木及農(nóng)作物等,造成嚴重的經(jīng)濟損失。廣東地處南亞熱帶季風氣候區(qū),氣候溫暖潮濕,麗赤殼屬真菌所致病害嚴重。為明確麗赤殼屬種類,為廣州市麗赤殼屬真菌病害防控提供理論依據(jù),本論文對廣州及周邊地區(qū)的麗赤殼屬真菌進行了調(diào)查采樣和分離,通過形態(tài)學和分子生物學方法對分離獲得的菌株進行了種類鑒定,并分析其系統(tǒng)發(fā)育關系,主要研究結果如下:從廣州地區(qū)的植物上共采集分離得到16個麗赤殼屬真菌菌株。在觀察標準培養(yǎng)條件下形態(tài)學特征的基礎上,結合肌動蛋白(ACT)、鈣調(diào)蛋白(CAL)、組蛋白H3(HIS3)、翻譯延伸因子(TEF-1α)和β-微管蛋白(TUB2)共5個基因序列的系統(tǒng)發(fā)育分析,將16個菌株鑒定為5個復合群中11個種:Calonectria cylindrospora復合群中的天南星生麗赤殼Ca.spathiphyllicola、紅千層麗赤殼Ca.callistemonii、天南星麗赤殼Ca.spathiphylli、霍奇斯麗赤殼Ca.hodgesii;Ca.brassicae復合群中的東方麗赤殼Ca.orientalis;Ca.reteaudii復合群中的野牡丹麗赤殼Ca.tibouchinae、五隔大無性孢麗赤殼Ca.pentaseptata、小無性孢麗赤殼Ca.microconidialis;Ca.colhounii復合群中的克儒斯麗赤殼Ca.crousiana;Ca.kyotensis復合群中的香港麗赤殼Ca.hongkongensis、冬青麗赤殼Ca.ilicicola。其中,Ca.spathiphyllicola、Ca.callistemonii、Ca.tibouchinae為新種,Ca.orientalis和Ca.hodgesii為中國新記錄種。簽名樹(Clusia rosea)、野牡丹(Melastoma sp.)為麗赤殼屬真菌新寄主,薔薇(Rosa sp.)為Ca.hodgesii新寄主,紅千層(Callistemon rigidus)為Ca.pentaseptata、Ca.crousiana新寄主,千層金(Melaleuca bracteata)為Ca.pentaseptata新寄主,鶴望蘭(Strelitzia reginae)為Ca.microconidialis新寄主,番荔枝(Annona squamosa)和鳳梨(Ananas sp.)為Ca.ilicicola新寄主。所鑒定菌株均附有菌落特征、顯微形態(tài)圖和形態(tài)特征描述。研究發(fā)現(xiàn),16個麗赤殼屬菌株形態(tài)存在豐富的多樣性,僅依靠形態(tài)特征難以正確區(qū)分其種類。本研究通過形態(tài)學結合ACT、HIS3、TEF-1α、CAL和TUB2等5個基因聯(lián)合序列系統(tǒng)發(fā)育樹分析,能有效地將16個菌株鑒定到種的水平。5個基因中,ACT基因對麗赤殼屬真菌物種的鑒別能力最弱,而TEF-1α基因對復合群中相似種的劃分最有效。
[Abstract]:(Calonectria) fungi are a kind of important plant pathogens, which can damage trees and crops and cause serious economic losses. Guangdong is located in the subtropical monsoon climate, the climate is warm and humid. In order to identify the species of Rhizoma, and to provide a theoretical basis for the prevention and control of Rhizoma fungi in Guangzhou, this paper investigated and isolated the fungi from Guangzhou and its surrounding areas. The species identification and phylogenetic relationship of the isolated strains were studied by morphological and molecular biological methods. The main results were as follows: a total of 16 strains were isolated from plants in Guangzhou. On the basis of observation of morphological characteristics under standard culture conditions, actin (ACT), calmodulin (CAL), histone H3 (HIS3) was combined with actin. Phylogenetic analysis of 5 gene sequences of translation extension factor (TEF-1 偽) and 尾 -tubulin (TUB2), 16 strains were identified as Ca.spathiphyllicola, in 11 species of: Calonectria cylindrospora complex of 5 complex groups. Red thousand layer red shell Ca.callistemonii, star red shell Ca.spathiphylli, Hodgsly red shell Ca.hodgesii; The Red Shell Ca.crousiana; in the Oriental Red Shell Ca.orientalis;Ca.reteaudii complex of the Ca.brassicae complex Ca.tibouchinae, in the Ca.pentaseptata, small asexual Red Shell Ca.microconidialis;Ca.colhounii complex Hong Kong Red Shell Ca.hongkongensis, in Ca.kyotensis complex Group Ca.ilicicola. Among them, Ca.spathiphyllicola,Ca.callistemonii,Ca.tibouchinae is a new species, Ca.orientalis and Ca.hodgesii are new records of China. Signature tree (Clusia rosea), wild peony (Melastoma sp.) Rosa (Rosa sp.) is a new host of Rhizoma. It is a new host of Ca.hodgesii, a new host of Ca.pentaseptata,Ca.crousiana, a new host of (Melaleuca bracteata), a new host of Ca.pentaseptata, a new host of Ca.microconidialis, (Annona squamosa) of Annona and (Ananas sp.) of pineapple. It is a new host of Ca.ilicicola. All the strains were characterized by colony characteristics, microscopic morphology and morphological characteristics. It was found that there was abundant diversity in the morphology of 16 strains of the genus Ceratopsis, and it was difficult to distinguish their species correctly depending on their morphological characteristics. In this study, by combining morphological analysis with ACT,HIS3,TEF-1 偽, CAL and TUB2 gene sequence phylogenetic tree analysis, 16 strains can be effectively identified to the level of the species. ACT gene had the weakest ability to identify the species of Lycidium, while TEF-1 偽 gene was the most effective for the classification of similar species in the complex group.
【學位授予單位】：仲愷農(nóng)業(yè)工程學院
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S432.44
本文編號：2341686
[Abstract]:(Calonectria) fungi are a kind of important plant pathogens, which can damage trees and crops and cause serious economic losses. Guangdong is located in the subtropical monsoon climate, the climate is warm and humid. In order to identify the species of Rhizoma, and to provide a theoretical basis for the prevention and control of Rhizoma fungi in Guangzhou, this paper investigated and isolated the fungi from Guangzhou and its surrounding areas. The species identification and phylogenetic relationship of the isolated strains were studied by morphological and molecular biological methods. The main results were as follows: a total of 16 strains were isolated from plants in Guangzhou. On the basis of observation of morphological characteristics under standard culture conditions, actin (ACT), calmodulin (CAL), histone H3 (HIS3) was combined with actin. Phylogenetic analysis of 5 gene sequences of translation extension factor (TEF-1 偽) and 尾 -tubulin (TUB2), 16 strains were identified as Ca.spathiphyllicola, in 11 species of: Calonectria cylindrospora complex of 5 complex groups. Red thousand layer red shell Ca.callistemonii, star red shell Ca.spathiphylli, Hodgsly red shell Ca.hodgesii; The Red Shell Ca.crousiana; in the Oriental Red Shell Ca.orientalis;Ca.reteaudii complex of the Ca.brassicae complex Ca.tibouchinae, in the Ca.pentaseptata, small asexual Red Shell Ca.microconidialis;Ca.colhounii complex Hong Kong Red Shell Ca.hongkongensis, in Ca.kyotensis complex Group Ca.ilicicola. Among them, Ca.spathiphyllicola,Ca.callistemonii,Ca.tibouchinae is a new species, Ca.orientalis and Ca.hodgesii are new records of China. Signature tree (Clusia rosea), wild peony (Melastoma sp.) Rosa (Rosa sp.) is a new host of Rhizoma. It is a new host of Ca.hodgesii, a new host of Ca.pentaseptata,Ca.crousiana, a new host of (Melaleuca bracteata), a new host of Ca.pentaseptata, a new host of Ca.microconidialis, (Annona squamosa) of Annona and (Ananas sp.) of pineapple. It is a new host of Ca.ilicicola. All the strains were characterized by colony characteristics, microscopic morphology and morphological characteristics. It was found that there was abundant diversity in the morphology of 16 strains of the genus Ceratopsis, and it was difficult to distinguish their species correctly depending on their morphological characteristics. In this study, by combining morphological analysis with ACT,HIS3,TEF-1 偽, CAL and TUB2 gene sequence phylogenetic tree analysis, 16 strains can be effectively identified to the level of the species. ACT gene had the weakest ability to identify the species of Lycidium, while TEF-1 偽 gene was the most effective for the classification of similar species in the complex group.
【學位授予單位】：仲愷農(nóng)業(yè)工程學院
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S432.44
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相關碩士學位論文 前1條
1 殷瑜;廣州地區(qū)麗赤殼屬真菌的種類鑒定[D];仲愷農(nóng)業(yè)工程學院;2016年
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