本文選題：象耳豆根結(jié)線蟲(chóng) + 促分裂原活化蛋白激酶��； 參考：《熱帶作物學(xué)報(bào)》2016年10期

【摘要】：促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是線蟲(chóng)體內(nèi)信號(hào)轉(zhuǎn)導(dǎo)的重要組分,在生長(zhǎng)發(fā)育過(guò)程中具有重要作用。本研究從象耳豆根結(jié)線蟲(chóng)中克隆了1個(gè)促分裂原活化蛋白激酶基因Me-mapk1c DNA序列,該序列全長(zhǎng)1 369 bp,包含1個(gè)1 185 bp的完整開(kāi)放閱讀框,推導(dǎo)編碼394個(gè)氨基酸,蛋白質(zhì)分子量為45.39 ku。同源性搜索比對(duì)結(jié)果發(fā)現(xiàn),該基因編碼的蛋白序列與南方根結(jié)線蟲(chóng)MI-MAPK1具有99%的一致性。通過(guò)構(gòu)建原核表達(dá)載體p ET-32a-MAPK,在IPTG的誘導(dǎo)下得到70 ku左右的特異融合蛋白(包括大小約26 ku的標(biāo)簽序列)。利用RNAi技術(shù)對(duì)象耳豆根結(jié)線蟲(chóng)二齡幼蟲(chóng)Me-mapk1基因進(jìn)行沉默,線蟲(chóng)誘導(dǎo)番茄根結(jié)形成的數(shù)量顯著減少,推測(cè)Me-mapk1基因的下調(diào)表達(dá)有可能影響象耳豆根結(jié)線蟲(chóng)二齡幼蟲(chóng)(J2)的生長(zhǎng)發(fā)育。
[Abstract]:Mitogen-activated protein kinase (MAPK), an important component of signal transduction in nematodes, plays an important role in the growth and development of the nematode. This study has cloned 1 mitogen activated protein kinase gene Me-mapk1c DNA sequences from the Elephant Ear bean root knot nematode, which has a full length of 1369 BP, including 1 1185. The complete open reading frame of BP, which encodes 394 amino acids, and the protein molecular weight of 45.39 ku. homologous search results, found that the protein sequence of the gene is consistent with the 99% of the southern root knot nematode MI-MAPK1. By constructing the prokaryotic expression vector p ET-32a-MAPK, the specific fusion egg of about 70 Ku is obtained under the induction of IPTG. White (including the size of the label sequence of about 26 KU). The RNAi technique was used to silence the Me-mapk1 gene of the two instar larvae of the root knot nematode. The number of root knot formation of the nematode induced tomato root formation was significantly reduced. It is speculated that the down regulation of the Me-mapk1 gene may affect the growth and development of the second instar larvae of the Elephant Ear bean root knot nematode (J2).
【作者單位】： 海南大學(xué)環(huán)境與植物保護(hù)學(xué)院;海南省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所海南省植物病蟲(chóng)害防控重點(diǎn)實(shí)驗(yàn)室;中國(guó)熱帶農(nóng)業(yè)科學(xué)院環(huán)境與植物保護(hù)研究所;
【基金】：973計(jì)劃前期研究專項(xiàng)(No.2014CB160307) 國(guó)家自然科學(xué)基金項(xiàng)目(No.31360432) 海南省植物病蟲(chóng)害防控重點(diǎn)實(shí)驗(yàn)室開(kāi)放課題
【分類號(hào)】：S432.45

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 練冬梅;趙志祥;陳綿才;王會(huì)芳;;象耳豆根結(jié)線蟲(chóng)actin基因的克隆與分析[J];西南農(nóng)業(yè)學(xué)報(bào);2014年03期

，

本文編號(hào)：2095174