本文選題：象耳豆根結(jié)線蟲 + 促分裂原活化蛋白激酶　； 參考：《熱帶作物學(xué)報(bào)》2016年10期

【摘要】：促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是線蟲體內(nèi)信號(hào)轉(zhuǎn)導(dǎo)的重要組分,在生長(zhǎng)發(fā)育過程中具有重要作用。本研究從象耳豆根結(jié)線蟲中克隆了1個(gè)促分裂原活化蛋白激酶基因Me-mapk1c DNA序列,該序列全長(zhǎng)1 369 bp,包含1個(gè)1 185 bp的完整開放閱讀框,推導(dǎo)編碼394個(gè)氨基酸,蛋白質(zhì)分子量為45.39 ku。同源性搜索比對(duì)結(jié)果發(fā)現(xiàn),該基因編碼的蛋白序列與南方根結(jié)線蟲MI-MAPK1具有99%的一致性。通過構(gòu)建原核表達(dá)載體p ET-32a-MAPK,在IPTG的誘導(dǎo)下得到70 ku左右的特異融合蛋白(包括大小約26 ku的標(biāo)簽序列)。利用RNAi技術(shù)對(duì)象耳豆根結(jié)線蟲二齡幼蟲Me-mapk1基因進(jìn)行沉默,線蟲誘導(dǎo)番茄根結(jié)形成的數(shù)量顯著減少,推測(cè)Me-mapk1基因的下調(diào)表達(dá)有可能影響象耳豆根結(jié)線蟲二齡幼蟲(J2)的生長(zhǎng)發(fā)育。
[Abstract]:Mitogen-activated protein kinase (MAPK), an important component of signal transduction in nematodes, plays an important role in the growth and development of the nematode. This study has cloned 1 mitogen activated protein kinase gene Me-mapk1c DNA sequences from the Elephant Ear bean root knot nematode, which has a full length of 1369 BP, including 1 1185. The complete open reading frame of BP, which encodes 394 amino acids, and the protein molecular weight of 45.39 ku. homologous search results, found that the protein sequence of the gene is consistent with the 99% of the southern root knot nematode MI-MAPK1. By constructing the prokaryotic expression vector p ET-32a-MAPK, the specific fusion egg of about 70 Ku is obtained under the induction of IPTG. White (including the size of the label sequence of about 26 KU). The RNAi technique was used to silence the Me-mapk1 gene of the two instar larvae of the root knot nematode. The number of root knot formation of the nematode induced tomato root formation was significantly reduced. It is speculated that the down regulation of the Me-mapk1 gene may affect the growth and development of the second instar larvae of the Elephant Ear bean root knot nematode (J2).
【作者單位】： 海南大學(xué)環(huán)境與植物保護(hù)學(xué)院;海南省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所海南省植物病蟲害防控重點(diǎn)實(shí)驗(yàn)室;中國(guó)熱帶農(nóng)業(yè)科學(xué)院環(huán)境與植物保護(hù)研究所;
【基金】：973計(jì)劃前期研究專項(xiàng)(No.2014CB160307) 國(guó)家自然科學(xué)基金項(xiàng)目(No.31360432) 海南省植物病蟲害防控重點(diǎn)實(shí)驗(yàn)室開放課題
【分類號(hào)】：S432.45

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