本文選題：栗蘑菌渣 + 栗樹有機肥��； 參考：《華北理工大學》2017年碩士論文

【摘要】：以栗蘑栽培后的廢棄菌渣為基料和載體,配以枯草芽孢桿菌、地衣芽孢桿菌、膠質芽孢桿菌、側孢芽孢桿菌生物菌發(fā)酵,添加聚天冬氨酸、檸檬酸的配合物,增加微量元素硼等的使用,復配制備出利于栗樹生長的生物有機菌肥,研究有機菌肥對板栗增產的影響,對探索栗蘑菌渣的資源綜合利用具有重要意義。測定了不同類型的栗樹土壤主要元素的含量,并分析其與板栗產量的關系。結果表明:有效硼、硝態(tài)氮、有效磷、有效鐵、有效錳、有效鈣含量分別在1.1～1.8mg/kg,21.4～32.8 mg/kg,80.1～121.2 mg/kg,31.6～47.8 mg/kg,41.0～65.9 mg/kg,3.2～3.7 g/kg時,板栗產量較高;硝態(tài)氮含量高于42.1 mg/kg,有效的硼含量低于0.9 mg/kg、磷含量低于36.4 mg/kg、鐵含量低于20.0 mg/kg、錳含量低于40.0mg/kg時,板栗產量較低。栗蘑菌渣的主要成分含量測定發(fā)現,N、P、K含量分別占栗蘑菌渣的0.50%、0.56%、1.10%,B、Zn、Ca、Mg、Fe、Mn含量均高于0.013%,可作為復配生物有機菌肥的基本原料。栗蘑菌渣SEM分析表明:屬于疏松多孔結構,可以滿足生物有機菌肥中使用的菌種載體的要求。確定了四種芽孢桿菌在菌渣中的最佳接種條件:菌液與菌渣體積比為25%,34℃條件下培養(yǎng)60 h。研究了聚天冬氨酸與錳、鐵、鈣絡合以及檸檬酸與硼絡合的反應條件,合成了能夠改善土壤肥力,容易被栗樹吸收的聚天冬氨酸錳、聚天冬氨酸鐵、聚天冬氨酸鈣和檸檬酸硼。確定了8～10年生栗樹所需生物有機菌肥配比:1332 kg的菌渣,添加枯草桿菌菌液、地衣芽孢桿菌菌液、膠質芽孢桿菌菌液、側孢芽孢桿菌菌液各550 mL,復配檸檬酸硼0.82 kg,聚天冬氨酸錳22.44 kg,聚天冬氨酸鐵14.52 kg,聚天冬氨酸鈣24.24 kg,60%含水量混合均勻繼續(xù)發(fā)酵,60 h完成。選用不同樹齡的24棵栗樹進行應用小試,其結果:施加復配肥料后的高、低產組栗樹產量普遍較上一年分別提升了13.75%、14.46%,相較于同年未施加肥料的栗樹凈增長分別為37.53%、38.37%,驗證了生物有機菌肥的肥效。
[Abstract]:The waste fungus after the mushroom cultivation was used as the base material and carrier, with Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus spore bacillus, adding polyaspartic acid, citric acid complex and increasing the use of trace element boron to prepare bio organic bacterial fertilizer for the growth of chestnut, and study organic bacteria. The effect of fertilizer on the increase of chestnut yield is of great significance to the exploration of the comprehensive utilization of the resources of mushroom residue. The content of the main elements in different types of chestnut soil and its relationship with the yield of chestnut are determined. The results show that the effective boron, nitrate nitrogen, effective phosphorus, effective iron, effective manganese and effective calcium content are from 1.1 to 1.8mg/kg, 21.4 to 32.8 m, respectively. G/kg, 80.1 ~ 121.2 mg/kg, 31.6 ~ 47.8 mg/kg, 41 ~ 65.9 mg/kg, 3.2 to 3.7 g/kg, the yield of chestnut is higher, the content of nitrate nitrogen is higher than 42.1 mg/kg, the effective boron content is lower than 0.9 mg/kg, the content of phosphorus is less than 36.4 mg/kg, the content of iron is lower than 20 mg/kg, and the yield of chestnut is lower than 40.0mg/kg. The main composition of the fungus of chestnut mushroom residue is measured. It was found that the content of N, P and K accounted for 0.50%, 0.56%, 1.10%, 1.10%, B, Zn, Ca, Mg, Fe, Mn content above 0.013% respectively, and could be used as the basic raw material for the compound biological organic manure. The SEM analysis of mushroom residue in chestnut fungus showed that it belonged to the porous structure and could meet the requirements of the bacteria carrier used in biological organic manure. The optimum conditions for inoculation in the bacteria residue: the volume ratio of bacterial and bacterial residue was 25%, and the culture of 60 h. at 34 C was used to study the reaction conditions of the complexation of polyaspartic acid and manganese, iron, calcium and citric acid and boron, and the synthesis of polyaspartic manganese, polyaspartate, polyaspartic calcium and lemon, which could improve soil fertility and easily be absorbed by chestnut trees. Boron acid. The ratio of bio organic fertilizer for 8~10 year old chestnuts: 1332 kg bacteria residue, Bacillus subtilis liquid, Bacillus licheniformis, Bacillus cereus, Bacillus spore bacillus, 550 mL, 0.82 kg of boron citrate, 22.44 kg of polyaspartic acid manganese, 14.52 kg of polyaspartic acid, 24.24 kg, 6 of polyasparin, 6 The 0% water content was mixed and continued to ferment and 60 h was completed. 24 chestnut trees of different age were selected for application. The results showed that the yield of chestnut increased by 13.75% and 14.46% respectively after the application of compound fertilizer. The net growth of chestnut trees that did not apply fertilizer in the same year was 37.53% and 38.37% respectively, and the biological organic bacteria were verified. Fertilizer efficiency.
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TQ446

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