本文選題：水平基因轉(zhuǎn)移 + 自然轉(zhuǎn)化��； 參考：《華中農(nóng)業(yè)大學(xué)學(xué)報(bào)》2016年02期

【摘要】：為確定土壤中的DNA通過自然轉(zhuǎn)化的方式發(fā)生水平基因轉(zhuǎn)移的頻率,將1個帶有egfp和卡那霉素抗性基因Kanr的質(zhì)粒DNA添加到自然土壤中,分離菌懸液,上流式細(xì)胞儀分選能發(fā)出綠色熒光且具有卡那霉素抗性的陽性轉(zhuǎn)化子,確定土壤中發(fā)生自然轉(zhuǎn)化的頻率小于10~(-7)。分離的菌懸液通過培養(yǎng)基的富集,從39 736 426個細(xì)胞中分選獲得了82個細(xì)胞,最終得到了純化的陽性轉(zhuǎn)化子,證實(shí)土壤中的DNA通過自然轉(zhuǎn)化發(fā)生了水平基因轉(zhuǎn)移。
[Abstract]:In order to determine the frequency of horizontal gene transfer of DNA in soil by natural transformation, a plasmid DNA containing egfp and kanamycin resistant gene Kanr was added to natural soil to isolate bacterial suspension. The up-flow cytometer was used to separate the positive transformants with green fluorescence and kanamycin resistance, and the frequency of natural transformation in soil was determined to be less than 10 ~ (10) ~ (-7) ~ (th). 82 cells were isolated from 39 736 426 cells and purified positive transformants were obtained, which confirmed that horizontal gene transfer took place through natural transformation of DNA in soil.
【作者單位】： 華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)微生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(41071180,41230854) 國家“863”計(jì)劃項(xiàng)目(2012AA101402)
【分類號】：S154
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本文編號：1882535