本文選題：高通量測序 + 新型肥料增效劑��； 參考：《微生物學(xué)通報》2017年11期

【摘要】：【目的】高通量測序技術(shù)對研究環(huán)境樣品中微生物群落組成具有很大的應(yīng)用價值。土壤微生物群落結(jié)構(gòu)和多樣性及其變化在一定程度上反映了土壤的質(zhì)量。旨在從微生物群落結(jié)構(gòu)的角度闡述環(huán)保肥料增效劑對馬鈴薯根際土壤主要真菌類群結(jié)構(gòu)的影響�！痉椒ā客ㄟ^高通量測序結(jié)果對比分析應(yīng)用增效劑前后馬鈴薯根際真菌宏基因組ITS1區(qū),并依據(jù)RDP中設(shè)置的分類閾值對序列進行物種分類�！窘Y(jié)果】測序結(jié)果經(jīng)過質(zhì)量控制,共獲得有效條帶437 375條,依據(jù)97%的序列相似性做聚類分析,獲得全部樣品的可分類操作單元(OTUs)共633個。子囊菌的總體數(shù)量在所有樣品中最多(相對豐度在56.95%-97.23%之間),且處理后呈增加趨勢(HY除外),而擔(dān)子菌門數(shù)量在處理后呈下降的趨勢。基于真菌ITS1高通量測序結(jié)果獲得的α指數(shù)顯示,樣品內(nèi)部處理和對照之間真菌物種多樣性有差別。基于真菌ITS1高通量測序獲得的β指數(shù)顯示,處理組與對照組的真菌多樣性之間沒有差別,這表明真菌多樣性之間的差異更多地取決于樣品采集地點�！窘Y(jié)論】土壤特性是影響真菌種群的重要因素之一,環(huán)保肥料增效劑顯著改善了土壤真菌的種群結(jié)構(gòu)。
[Abstract]:[objective] High-throughput sequencing technique has great application value in the study of microbial community composition in environmental samples. The structure and diversity of soil microbial communities and their changes reflect the soil quality to some extent. The purpose of this paper is to expound the effect of environmental fertilizer synergist on the structure of main fungi in potato rhizosphere soil from the point of view of microbial community structure. [methods] Potato roots before and after application of synergist were analyzed by high-throughput sequencing results. The ITS1 region of the Macrogenome of International Fungi, The sequence was classified according to the classification threshold set in RDP. [results] after quality control, a total of 437,375 effective bands were obtained. Cluster analysis was made according to 97% sequence similarity. The total number of classifiable operating units (OTUs) of all samples was 633. The total quantity of ascomycetes was the highest in all samples (relative abundance was between 56.95% and 97.23%), and the amount of basidiomycetes decreased after treatment. The 偽 -index obtained from the high throughput sequencing of fungal ITS1 showed that the species diversity of fungi was different between the internal treatment and the control. The 尾 index based on high-throughput sequencing of fungal ITS1 showed that there was no difference in fungal diversity between the treatment group and the control group. [conclusion] soil characteristics are one of the important factors affecting the fungal population, and the synergist of environmental fertilizer can improve the population structure of soil fungi significantly.
【作者單位】： 中國科學(xué)院微生物研究所傳感技術(shù)聯(lián)合國家重點實驗室;中國科學(xué)院微生物研究所植物基因組學(xué)國家重點實驗室;寧夏現(xiàn)代農(nóng)業(yè)綜合開發(fā)工程技術(shù)研究中心;寧夏自治區(qū)農(nóng)業(yè)綜合開發(fā)辦公室;寧夏海原縣農(nóng)業(yè)綜合開發(fā)辦公室;寧夏農(nóng)墾賀蘭山種業(yè)有限公司;
【基金】：中國科學(xué)院重點部署項目(No.KFJ-EW-STS-067)~~
【分類號】：S154.3;S532

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