本文關(guān)鍵詞： 蘋果內(nèi)生菌 拮抗作用 抑菌機(jī)制 鑒定　出處：《微生物學(xué)通報》2016年10期 　論文類型：期刊論文

【摘要】：【目的】鑒定一株分離自蘋果健康枝條的內(nèi)生菌long A,對其抑菌作用機(jī)理進(jìn)行初步探究。【方法】通過生理生化性質(zhì)測定和16S r RNA基因序列分析對其進(jìn)行鑒定;采用平板對峙法檢測long A對蘋果樹腐爛病菌(Valsa mali)菌絲生長的影響和對孢子萌發(fā)的抑制作用,利用透射電鏡觀察蘋果樹腐爛病菌的細(xì)胞內(nèi)部變化�！窘Y(jié)果】16S r RNA基因序列分析結(jié)果表明該菌株為解淀粉芽孢桿菌Bacillus amyloliquefaciens;菌株long A好氧,可利用葡萄糖、蔗糖、甘露醇、乳酸、檸檬酸等碳源;耐鹽性差(不能耐受質(zhì)量分?jǐn)?shù)7%的氯化鈉);接觸酶反應(yīng)呈陽性;可還原硝酸鹽;能使明膠液化、水解淀粉;不能利用檸檬酸鹽;乙酰甲基甲醇(V-P)試驗為陰性。long A能顯著地降低蘋果樹腐爛病菌分生孢子的萌發(fā)率,并導(dǎo)致其菌絲生長過程中分枝增多、頂端膨大及細(xì)胞質(zhì)外滲�！窘Y(jié)論】菌株long A能有效抑制蘋果樹腐爛病菌等,具有一定的生防潛能。
[Abstract]:[objective] to identify a strain of endophytic bacteria long A isolated from healthy branches of apple, and to explore its bacteriostatic mechanism. [methods] it was identified by physiological and biochemical properties and 16s r RNA gene sequence analysis. The effects of long A on the growth of apple tree rot fungus Valsa mali) and its inhibition on spore germination were detected by using the plate confrontation method. [results] the 16s r RNA gene sequence analysis showed that the strain was Bacillus amylolique faciens, strain long A was aerobic, and could use glucose, sucrose, mannitol, lactic acid. Keywords carbon source such as citric acid; poor salt tolerance (intolerant to sodium chloride with 7% mass fraction; positive contact enzyme reaction; reducing nitrate; liquefaction of gelatin, hydrolysis of starch; no use of citrate; Acetylmethyl methanol-V-P) test showed that negative. Long A could significantly reduce the germination rate of conidia of apple tree rot bacteria, and lead to the increase of branches during the growth of mycelium. [conclusion] strain long A can effectively inhibit apple tree rot bacteria and has certain biocontrol potential.
【作者單位】： 旱區(qū)作物逆境生物學(xué)國家重點實驗室;西北農(nóng)林科技大學(xué)生命科學(xué)學(xué)院;西北農(nóng)林科技大學(xué)植物保護(hù)學(xué)院;
【基金】：國家自然科學(xué)基金項目(No.31101476,31171796) 陜西省科學(xué)技術(shù)研究發(fā)展計劃項目(No.2013K01-45) 楊凌示范區(qū)科技計劃項目(No.2014NY-41)~~
【分類號】：S436.611.11;S476.1
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本文編號：1531163