【摘要】：以堿性產(chǎn)氨菌Providencia Jat-1.為原始菌種,使用鹽酸羥胺對其進(jìn)行化學(xué)誘變,考察誘變前后細(xì)菌活性、形貌以及浸銅能力的變化。研究結(jié)果表明:鹽酸羥胺的誘變作用提高細(xì)菌的活性以及浸銅能力。1.5%(質(zhì)量分?jǐn)?shù))的鹽酸羥胺為最佳誘變劑含量,在此條件下細(xì)菌的致死率為81.00%、正突變率為16.67%。與誘變前細(xì)菌相比,誘變后細(xì)菌生長到達(dá)穩(wěn)定期時間縮短10 h,穩(wěn)定期細(xì)菌濃度提高30.00%,產(chǎn)氨量提高17.60%,誘變后細(xì)菌尺寸與形狀未發(fā)生變化,但其表面分泌物增多。使用誘變菌種浸出銅礦石,168 h后銅浸出率達(dá)58.52%,比誘變前細(xì)菌浸出率提高10.21%。浸出后礦石表面細(xì)顆粒礦物消失,礦石表面未形成結(jié)晶或沉淀,表面孔裂隙明顯增多。
[Abstract]:Providencia Jat-1. of Alkaline ammonia producing bacteria Hydroxylamine hydrochloride was used for chemical mutagenesis to investigate the changes of bacterial activity, morphology and copper leaching ability before and after mutagenesis. The results showed that the mutagenic effect of hydroxylamine hydrochloride increased the activity of bacteria and the concentration of hydroxylamine hydrochloride with copper leaching ability of 1.5% (mass fraction) was the best. Under this condition, the mortality of bacteria was 81.00 and the positive mutation rate was 16.67%. Compared with the bacteria before mutagenesis, the growth time of bacteria after mutagenesis was shortened by 10 hours, the concentration of bacteria in stable period was increased by 30.00000 and ammonia production was increased by 17.600.After mutagenesis, the size and shape of bacteria did not change, but the surface secretion increased. The leaching rate of copper from copper ore by mutagenesis bacteria was 58.52 after 168 h, which was 10.21 higher than that before mutagenesis. After leaching, fine grained minerals disappeared, and no crystallization or precipitation was formed on the surface of the ore, and the number of pore cracks on the surface increased obviously.
【作者單位】： 北京科技大學(xué)金屬礦山高效開采與安全教育部重點實驗室;
【基金】：國家“十二五”科技支撐計劃項目(2012BAB08B02) 國家自然科學(xué)基金資助項目(51304011,51374035)~~
【分類號】：TD952
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本文編號：2237638