可識(shí)別單一核苷酸錯(cuò)配的微懸臂基因檢測(cè)技術(shù)力學(xué)模型（英文）
發(fā)布時(shí)間：2025-03-30 05:58
基于表面種植有DNA分子層的微懸臂梁系統(tǒng),在考慮該帶電DNA分子空間構(gòu)型與其表面分布影響的基礎(chǔ)上,提出了新的力學(xué)模型以定量描述完全與不完全雜交情形下導(dǎo)致的微懸臂梁撓曲變形.該模型將撓度曲率半徑作為反饋DNA檢測(cè)信號(hào)的唯一變量.預(yù)測(cè)出的完全與不完全DNA雜交情形與懸臂梁撓曲變形之間的關(guān)系與文獻(xiàn)[2]的實(shí)驗(yàn)觀測(cè)相符,且預(yù)測(cè)精度可滿足單一堿基對(duì)變化的檢測(cè)需要,對(duì)高精確DNA檢測(cè)技術(shù)的開發(fā)具有借鑒意義.
【文章頁(yè)數(shù)】：11 頁(yè)
【文章目錄】：
1Materials and methods
1.1 Initial deflection of the cantilever with im-mobilized ssDNAs
1.2 Deflection induced by DNA hybridization
1.3 Mismatch from incomplete hybridization
1.4 Mismatch from the mutation of targets
2 Results
2.1 Parameters with changing grafting density
2.2 Immobilization of probe ssDNAs
2.3 Analysis of complete hybridization and exter-nal mismatches.
2.4 Deflection variations with different PB con-centrations.
2.5 Mutation causes internal mismatches
Appendix
本文編號(hào)：4038297
【文章頁(yè)數(shù)】：11 頁(yè)
【文章目錄】：
1Materials and methods
1.1 Initial deflection of the cantilever with im-mobilized ssDNAs
1.2 Deflection induced by DNA hybridization
1.3 Mismatch from incomplete hybridization
1.4 Mismatch from the mutation of targets
2 Results
2.1 Parameters with changing grafting density
2.2 Immobilization of probe ssDNAs
2.3 Analysis of complete hybridization and exter-nal mismatches.
2.4 Deflection variations with different PB con-centrations.
2.5 Mutation causes internal mismatches
Appendix
本文編號(hào)：4038297
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