目的:探討微小RNA 1827(mi R-1827)對甲狀腺乳頭狀癌細胞增殖、侵襲轉(zhuǎn)移及對c-Myc基因表達的影響。方法:采用實時熒光定量聚合酶鏈反應(yīng)(q PCR)檢測mi R-1827在TPC-1和Nthy-ori 3-1細胞中的表達,分別用CCK-8、細胞侵襲和蛋白質(zhì)印跡法(Western blot)檢測過表達及干擾mi R-1827對TPC-1細胞增殖、侵襲和上皮間質(zhì)轉(zhuǎn)化(EMT)的影響。采用Target Scan和雙熒光素酶報告基因?qū)嶒灧治鰉i R-1827與c-Myc之間的作用靶點,通過q PCR和Western blot檢測mi R-1827和c-Myc之間的調(diào)控關(guān)系。通過Western blot分析mi R-1827對TPC-1細胞中Akt-m TOR通路的影響。雷帕霉素抑制Akt-m TOR通路后,檢測mi R-1827對甲狀腺乳頭狀癌細胞增殖、侵襲轉(zhuǎn)移以及對c-Myc基因表達的影響。結(jié)果:TPC-1細胞中mi R-1827表達水平明顯低于Nthy-ori 3-1細胞(P<0.01),過表達mi R-1827能夠抑制TPC-1細胞的增殖和侵襲(P<0.05)...

【文章頁數(shù)】：9 頁

【文章目錄】：
Introduction
Materials and methods
    Main materials
Methods
    Cell culture
    Cell transfection
    Cell proliferation assay
    Detection of dual luciferase reporter gene activity
    Cell invasion assay
    RT-q PCR
    Western blot
    Statistical method
Results
    Overexpression of mi R-1827 inhibited TPC-1 cell proliferation,invasion and epithelial-mesenchymal transition(EMT)
    Down-regulation of mi R-1827 promotes proliferation,invasion and EMT of TPC-1 cell
    Mi R-1827 regulates proliferation,invasion and EMT of TPC-1 cell through Akt-m TOR pathway
    Targeting and regulating relationship between mi R-1827 and c-Myc
    Mi R-1827 regulates c-Myc expression through Akt-m TOR pathway
Conclusion



本文編號：3809096