本文關(guān)鍵詞：Waxy基因的RNA沉默使轉(zhuǎn)基因小麥種子中直鏈淀粉含量下降(英文)，由筆耕文化傳播整理發(fā)布。

    RNA Silencing and Its Application in Plant Gene Function Research and Crop Improvement

    RNA沉默及其在植物基因功能研究和作物改良中的應(yīng)用

短句來源

    RNA Silencing of Waxy Gene Results in Low Levels of Amylose in the Seeds of Transgenic Wheat (Triticum aestivum L.)

    Waxy基因的RNA沉默使轉(zhuǎn)基因小麥種子中直鏈淀粉含量下降(英文)

短句來源

    Comparison of RNA Silencing Inducing Efficiency of Three PVX CP Gene Constructs via Transient Expression

    瞬時表達比較馬鈴薯X病毒CP基因3種結(jié)構(gòu)對RNA沉默的誘導(dǎo)效果

短句來源

    The influence of temperature on RNA silencing and the level of resistance in transgenic tobacco plants was studied by using Northern blotting and ELISA to detect the virus in T3 plants that contain untranslatable PVYN CP gene.

    本研究以轉(zhuǎn)不可翻譯的馬鈴薯Y病毒壞死株系外殼蛋白基因(PVYN CP)煙草的T3代植株為材料,在獲得高度抗病植株并證明轉(zhuǎn)基因植株的抗病性是由RNA沉默介導(dǎo)的基礎(chǔ)上,采用Northern雜交及ELISA檢測病毒的方法,分析了溫度對轉(zhuǎn)基因煙草中RNA沉默以及轉(zhuǎn)基因植株抗病水平的影響。

短句來源

    In this study, in order to effectively transcribe dsRNA in plants, an inducer of RNA silencing, two copies of Tobacco mosaic virus (TMV) partial movement protein gene (△MP) were ligated with soybean intron in inverted repeat manner, the recombinant was then inserted into binary vector pBIN438 under control of the 35S promoter.

    本研究以在植物體內(nèi)轉(zhuǎn)錄出RNA沉默的高效誘導(dǎo)因子—雙鏈RNA(dsRNA)為目標(biāo)，，將煙草花葉病毒(TMV)部分移動蛋白基因(ΔMP)以反向重復(fù)的方式與大豆內(nèi)含子相連，并定向插入到植物表達載體pBIN438上35S啟動子下游；

短句來源

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    Semi-quantitative RT-PCR assay demonstrated that NbTOM1 mRNA accumulation was inhibited in N. benthamiana. These results suggest that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants and virus induced gene silencing is is a useful method for identification of gene function.

    本論文證明通過沉默NbTOM1是一種有效的獲得抗TMV植株的方法,同時表明病毒誘導(dǎo)的基因沉默是一種切實有效的鑒定基因功能的工具。

短句來源

    These results could be used for further research of Rubisco subunit gene function and mechanism of RNA silencing.

    本實驗為進一步研究Rubisco小亞基基因功能和基因沉默機制奠定了基礎(chǔ)。

短句來源

    Plant expression vectors with untranslatable transgene arranged as sense, antisense orientation and inverted repeat were constructed with the full-length potato virus X (PVX) coat protein (CP) gene and their efficiency of RNA silencing inducing was analyzed after transient expression via agroinfiltration.

    用全長馬鈴薯X病毒(PVX)CP基因構(gòu)建非翻譯的正義、反義和反向重復(fù)結(jié)構(gòu)轉(zhuǎn)基因的植物表達載體,通過農(nóng)桿菌滲入法瞬時表達測試了這些轉(zhuǎn)基因?qū)NA介導(dǎo)抗性的誘導(dǎo)效率和有效性.

短句來源

  本文關(guān)鍵詞：Waxy基因的RNA沉默使轉(zhuǎn)基因小麥種子中直鏈淀粉含量下降(英文)，由筆耕文化傳播整理發(fā)布。