本文選題：等離子噴涂 + 生物材料　； 參考：《新疆大學(xué)》2015年碩士論文

【摘要】：隨著現(xiàn)代醫(yī)學(xué)的發(fā)展,組織替代和重建已經(jīng)成為人們關(guān)注的熱點,其關(guān)鍵性技術(shù)就是仿生材料的研發(fā)。Ti合金,羥基磷灰石(HA),聚乳酸(PLA)等是常用的生物材料。本文采取等離子噴涂工藝在Ti合金基體上噴涂制備生物壓電陶瓷復(fù)合涂層,將羥基磷灰石(HA)與鈦酸鋇(BaTiO3)壓電陶瓷復(fù)合制備生物活性材料,使其兼具生物活性與力電性能。Ti基體上等離子噴涂HA涂層已經(jīng)被廣泛研究,研究表明制備的涂層具有一定的生物性能和力學(xué)性能,但生物誘導(dǎo)活性并不高,在人體中也極容易剝落。為了進(jìn)一步提高涂層的性能,作者在前人的基礎(chǔ)上加入鈦酸鋇壓電陶瓷,希望涂層具有一定的壓電性能,利用骨生長的力電效應(yīng)原理,來改善生物涂層的誘導(dǎo)活性,通過控制等離子噴涂工藝參數(shù)來提高涂層與基體的結(jié)合性能。為了確定試驗最佳的工藝參數(shù),采用正交試驗設(shè)計合理的噴涂HA的工藝參數(shù),選取噴涂距離、噴涂電壓、噴涂電流為變量因素,噴涂電壓在60-70V,噴涂電流500-700A,噴涂距離80-120mm,以附著強度為考察因素,最終確定合適的工藝參數(shù)為噴涂距離80mm,噴涂電壓65V,噴涂電流600A,此時羥基磷灰石與Ti合金的附著強度達(dá)到最大值為25N。80-120mm內(nèi),隨著噴涂距離的增大,涂層的附著強度減小,60-70V內(nèi),隨著噴涂電壓的增大,涂層的附著強度先增大后減小,500-700A內(nèi),隨著噴涂電流的增大,涂層的附著強度先增大后減小。為了進(jìn)一步提高陶瓷涂層與金屬基體界面的潤濕性,在羥基磷灰石中加入純鈦粉,采取以上得到的最佳工藝參數(shù)噴涂制備Ti/HA涂層,最后再加入鈦酸鋇陶瓷粉末,采取同樣的工藝參數(shù)制備Ti/HA/Ba TiO3涂層。通過SEM/EDS對涂層進(jìn)行形貌分析和成分分析,用劃痕儀測試涂層與基體的附著強度,用Image J圖像處理軟件測試涂層孔隙率,通過對純Ti、Ti/HA涂層、Ti/HA/BaTiO3涂層進(jìn)行細(xì)胞培養(yǎng)試驗,在37℃,0.25%CO2濃度下分別培養(yǎng)1d,4d,7d后,用CCK-8法測試細(xì)胞增值率,評價細(xì)胞毒性,然后用掃描電鏡觀察細(xì)胞的黏附狀態(tài)。涂層SEM形貌結(jié)果表明:在噴涂距離80mm,噴涂電壓65V,噴涂電流600A的噴涂工藝下,制備的三種涂層具有層疊狀的組織結(jié)構(gòu),涂層熔化良好,有明顯的熔滴鋪展形貌�？紫堵蕼y試結(jié)果表明:涂層具有一定的微孔結(jié)構(gòu),孔隙率大約在2.6%-8.6%之間。通過劃痕試驗測試附著強度表明:最優(yōu)工藝參數(shù)下制備的三種涂層具有較高的附著強度,HA涂層為25N,Ti/HA涂層為35N,Ti/HA/BaTi O3涂層為27N,涂層與基體在界面處發(fā)生剝落失效。細(xì)胞毒性測試結(jié)果表明:純Ti、Ti/HA涂層的細(xì)胞等級為1級,Ti/HA/BaTiO3的細(xì)胞毒性等級趨于0級,能夠作為生物材料使用。通過對細(xì)胞的SEM形貌觀察,細(xì)胞在涂層上面附著良好,有較多的細(xì)胞偽足伸出。通過細(xì)胞增值率的比較,Ti/HA/Ba TiO3涂層的細(xì)胞增值率比其他兩種涂層的要高,這也說明了鈦酸鋇的加入對涂層的生物活性有了一定提高。以上結(jié)果表明,通過等離子噴涂工藝能夠制備具有一定附著強度的Ti/HA/BaTiO3復(fù)合涂層,利用壓電陶瓷的力電效應(yīng),能夠改善HA的生物誘導(dǎo)活性,為進(jìn)一步開發(fā)高性能的生物活性材料提供了參考依據(jù)。
[Abstract]:With the development of modern medicine, the replacement and reconstruction of tissue has become a hot spot of attention. The key technology is the research and development of biomimetic materials,.Ti alloys, hydroxyapatite (HA), polylactic acid (PLA) and so on as the common biomaterials. Hydroxyapatite (HA) and barium titanate (BaTiO3) piezoelectric ceramics are combined to prepare bioactive materials. The plasma spraying HA coating on.Ti substrate has been widely studied. The study shows that the coating has some biological and mechanical properties, but the bioactivity is not high, and it is also very important in the human body. In order to further improve the performance of the coating, the author added barium titanate piezoelectric ceramics on the basis of the predecessors. It is hoped that the coating has a certain piezoelectricity and the principle of the force and electricity effect of bone growth to improve the induction activity of the biological coating, and to improve the bonding performance of the coating and the substrate by controlling the parameters of the plasma spraying process. In order to determine the optimum technological parameters of the test, the orthogonal experiment was used to design the reasonable spraying process parameters of HA. The spraying distance, the spraying voltage and the spraying current were selected as the variables, the spraying voltage was 60-70V, the spraying current was 500-700A, the spraying distance was 80-120mm, and the adhesion strength was taken as the investigation factor. Finally, the suitable technological parameters were determined as the spraying distance. From 80mm, spraying voltage 65V and spraying current 600A, the adhesion strength of hydroxyapatite and Ti alloy reached the maximum value of 25N.80-120mm. With the increase of spray distance, the adhesion strength of the coating decreased, and the adhesion strength of the coating increased and then decreased with the increase of spraying voltage. In 500-700A, with the increase of spraying current, the coating was coated. The adhesion strength of the layer increases first and then decreases. In order to further improve the wettability of the interface between the ceramic coating and the metal matrix, the pure titanium powder is added to the hydroxyapatite, and the Ti/HA coating is prepared by the best technological parameters obtained above. Finally, the barium titanate ceramic powder is added, and the same process parameters are used to prepare the Ti/HA/Ba TiO3 coating. The coating was analyzed and analyzed by SEM/EDS. The adhesion strength of the coating and the substrate was tested by scratch tester. The porosity of the coating was tested by Image J image processing software. The cell culture test was carried out on pure Ti, Ti/HA coating and Ti/HA/BaTiO3 coating, and 1D, 4D and 7d were cultured at 37 degrees and 0.25%CO2 concentration, and the cell growth was tested by CCK-8 method. The adhesion status of cells was observed by scanning electron microscopy (SEM), and the morphology of the coated SEM showed that the three coatings prepared by spraying distance 80mm, spraying voltage 65V and spraying current 600A were stacked structure, the coating melted well, the droplet spreading morphology was obvious. The porosity test results were found. The results show that the coating has a certain microporous structure and the porosity is about 2.6%-8.6%. The adhesion strength of the three kinds of coatings prepared under the optimal process parameters shows that the coating has high adhesion strength, the HA coating is 25N, the Ti/HA coating is 35N, the Ti/HA/BaTi O3 coating is 27N, and the coating and the matrix are exfoliated at the interface. The results of toxicity test showed that the cell grade of pure Ti, Ti/HA coating was grade 1, the cytotoxicity grade of Ti/HA/BaTiO3 tended to 0, and could be used as biomaterial. By observing the SEM morphology of the cells, the cells were attached well on the coating and more cells were protruded out. Through comparison of cell value added rate, Ti/HA/Ba TiO3 coated cells The added value is higher than that of the other two kinds of coatings. It also shows that barium titanate has a certain increase in the biological activity of the coating. The above results show that the Ti/HA/BaTiO3 composite coating with certain adhesion strength can be prepared by plasma spraying process, and the bioactivity of HA can be improved by using the piezoelectric effect of the piezoelectric ceramic. It provides a reference for further developing high performance bioactive materials.

【學(xué)位授予單位】：新疆大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TG174.4

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本文編號：1788694