本文選題：事故傾向性 + 5-HT��； 參考：《廣西醫(yī)科大學(xué)》2016年碩士論文

【摘要】：目的通過廣西駕駛員血清中5-羥色胺(5-hydroxytryptamine,5-HT)、5-羥色氨酸(5-hydroxytryptophan,5-HTP)及其腦源性神經(jīng)營養(yǎng)因子(brain-derived neurotrophic factor, BDNF)含量的測(cè)定,尋求事故傾向性駕駛員的生物標(biāo)志物,探討事故傾向性駕駛員5-HT、5-HTP、BDNF水平與其發(fā)生事故的相關(guān)因素是否存在關(guān)聯(lián)。從生物學(xué)角度初步探索事故傾向性產(chǎn)生的原因和作用機(jī)制,為今后駕駛員的篩檢進(jìn)而減少交通事故的發(fā)生提供科學(xué)依據(jù)。方法采用病例對(duì)照的研究方法,通過調(diào)查問卷,根據(jù)研究對(duì)象的納入和排除標(biāo)準(zhǔn)選取2014年廣西事故傾向性駕駛員139例和正常對(duì)照駕駛員127例。通過高效液相色譜法(High performance liquid chromatography, HPLC)測(cè)定166例研究對(duì)象(病例組89例,對(duì)照組77例)血清中5-HT、 5-HTP水平。采用酶聯(lián)免疫吸附試驗(yàn)(enzyme linked immunosorbent assay, ELISA)測(cè)定100例研究對(duì)象(病例組和對(duì)照組各50例)血清BDNF水平。應(yīng)用EpiData3.1和SPSS16.0統(tǒng)計(jì)軟件進(jìn)行數(shù)據(jù)的錄入和統(tǒng)計(jì)分析。采用t檢驗(yàn)、χ2檢驗(yàn)、Pearson相關(guān)分析、非條件Logistic回歸分析的統(tǒng)計(jì)方法。結(jié)果 (1)病例組血清中5-HT (125.45±66.41)μg/L和5-HTP (225.06±55.54) μg/L均低于對(duì)照組5-HT (146.29±62.33) μg/L和5-HTP (249.33±58.16) μg/L (P0.05); 5-HT/5-HTP比值兩組間差異無統(tǒng)計(jì)學(xué)意義。(2)5-HTP與5-HT呈正相關(guān)趨勢(shì)(相關(guān)系數(shù)r=0.784,P0.05)；病例組中5-HTP與5-HT呈正相關(guān)趨勢(shì)(相關(guān)系數(shù)r--0.743,P0.05)；對(duì)照組中5-HTP與5-HT呈正相關(guān)趨勢(shì)(相關(guān)系數(shù)r=0.813,P0.05)。(3)駕齡至10年的駕駛員中,病例組5-HT(123.13±48.38)μg/L和5-HTP(219.65±45.98)μg/L均低于對(duì)照組5-HT(154.01±66.56)μg/L和5-HTP(254.00±46.85)μg/L(P0.05).(4)病例組中漢族駕駛員5-HTP(212.61±37.51)μg/L低于對(duì)照組中漢族駕駛員(246.37±44.39)μg/L(P0.05)；病例組中其他少數(shù)民族駕駛員5-HT(113.14±54.13)μg/L低于對(duì)照組中其他少數(shù)民族駕駛員(156.53±60.68)μg/L(P0.05)；病例組中漢族駕駛員5-HTP(212.61±37.51)μg/L低于病例組中其他少數(shù)民族駕駛員(241.98+70.83)μg/L(P0.05)。(5)非條件Logistic回歸結(jié)果顯示5-HIT、5-HTP和鍛煉頻率與事故傾向性有關(guān),可能為其保護(hù)因素。(6)病例組血清BDNF水平(3.18±2.19)ng/mL高于對(duì)照組水平(2.23±1.43)ng/mL(P0.05)；病例組駕齡10年的駕駛員血清BDNF水平高于對(duì)照組(P0.05)；病例組初中及以下的駕駛員血清BDNF水平高于對(duì)照組(P0.05)；病例組其他民族的駕駛員血清BDNF水平高于對(duì)照組(P0.05)。(7)非條件Logistic回歸結(jié)果顯示睡眠時(shí)間和鍛煉頻率與事故傾向性有關(guān),可能為其保護(hù)因素。結(jié)論血清5-HT、5.HTP、BDNF水平與事故傾向性駕駛員發(fā)生事故有關(guān)聯(lián)。血清5-HTP與5-HT呈正相關(guān)趨勢(shì),兩組血清5-HT/5-HTP比值沒有明顯差異,提示體內(nèi)血清5-HTP與5-HT的濃度比例變化一致。血清5-HT、 5-HTP、睡眠時(shí)間和鍛煉頻率可能為事故傾向性保護(hù)因素。
[Abstract]:Objective to explore the biomarkers of accident prone drivers by the determination of serotonin 5-hydroxytryptamine 5-hydroxytryptophanine 5-HTP (5-hydroxytryptophanine 5-HTP) and brain-derived neurotrophic factor (brain-derived neurotrophic factor, BDNF) in Guangxi drivers. To investigate the correlation between BDNF level and accident prone drivers. From the biological point of view, the causes and mechanism of accident tendency are preliminarily explored, which provides scientific basis for driver screening and reducing the occurrence of traffic accidents in the future. Methods 139 accident prone drivers in Guangxi in 2014 and 127 normal control drivers were selected according to the inclusion and exclusion criteria of the subjects. Serum 5-HT and 5-HTP levels were measured by high performance liquid chromatography (HPLC) in 166 subjects (89 cases in the case group and 77 cases in the control group). The serum BDNF levels were measured by enzyme linked immunosorbent assay, ELISA) in 100 subjects (50 cases in the case group and 50 cases in the control group) by enzyme linked immunosorbent assay (Elisa). EpiData3.1 and SPSS16.0 software are used to input and analyze the data. T test, 蠂 2 test, Pearson correlation analysis and non conditional Logistic regression analysis were used. Results (1) the serum levels of 5-HT and 5-HTP in the case group were 125.45 鹵66.41 渭 g / L and 225.06 鹵55.54 渭 g / L, respectively, which were lower than those in the control group (146.29 鹵62.33) 渭 g / L and 249.33 鹵58.16 渭 g / L respectively (P 0.05). There was no significant difference in 5-HT/5-HTP ratio between the two groups (r = 0.784, P 0.00. 05). In the control group, the trend of 5-HTP and 5-HT (r = 0.813) was positively correlated with that of the control group (r = 0.813, P 0.05, P 0.05, r = 0.74 3, P 0.05, P 0.05, r = 0.74 3, P 0.05, r = 0.74 3, P < 0.05). 鐥呬緥緇，

本文編號(hào)：1935947