本文選題：雙室無(wú)介體MFC + BOD傳感器�。� 參考：《北京林業(yè)大學(xué)》2015年碩士論文

【摘要】：生化需氧量(BOD)是表征有機(jī)物污染程度的綜合性指標(biāo)。傳統(tǒng)的五天生化需氧量(BOD5)為檢測(cè)BOD濃度的標(biāo)準(zhǔn)方法,但存在有耗時(shí)時(shí)間長(zhǎng)、結(jié)果重現(xiàn)性差等缺陷。微生物燃料電池(MFC)型BOD傳感器是一種快速檢測(cè)BOD5的新方法,具有穩(wěn)定性較強(qiáng)、檢測(cè)范圍廣等優(yōu)勢(shì)。本文以厭氧段污泥作接種物、質(zhì)子交換膜作陰陽(yáng)極室的分隔物構(gòu)建了雙室無(wú)介體MFC型BOD傳感器,并通過(guò)優(yōu)化陽(yáng)極材料和陰極電子受體等關(guān)鍵措施,改善MFC型BOD傳感器的檢測(cè)性能。BOD傳感器穩(wěn)定運(yùn)行后,通過(guò)模擬實(shí)際廢水初步研究了MFC型BOD傳感器檢測(cè)實(shí)際廢水的可行性,并利用PCR-DGGE技術(shù)分析了陽(yáng)極微生物群落結(jié)構(gòu)。采用硝酸酸化改性石墨氈電極。石墨氈酸化處理4h時(shí),表面接觸角變化最大。以硝酸酸化處理的石墨氈為電極材料、溶解氧為陰極電子受體構(gòu)建MFC型BOD傳感器,MFC優(yōu)化的檢測(cè)條件為：陰極進(jìn)水流量為30mL/min;廢水中緩沖溶液投加量為50mL/L;L-半胱氨酸投加量為20 mg/L；外接電阻為500Ω。BOD檢測(cè)范圍為2～100mg/L,響應(yīng)時(shí)間小于10 h,數(shù)據(jù)重現(xiàn)性良好。BOD濃度在2-50 mg/L范圍,可利用電流最大值檢測(cè)廢水BOD濃度；BOD濃度在2,～100mg/L范圍,可利用電荷量檢測(cè)廢水BOD濃度。以碳刷為電極材料,溶解氧為陰極電子受體構(gòu)建MFC型BOD傳感器,在已優(yōu)化的檢測(cè)條件下進(jìn)行BOD濃度的檢測(cè)。BOD濃度檢測(cè)范圍為2～100 mg/L,響應(yīng)時(shí)間小于12 h,數(shù)據(jù)重現(xiàn)性良好。BOD濃度在2-60 mg/L范圍,可利用電流峰值檢測(cè)廢水BOD濃度；BOD濃度在2～100mg/L范圍時(shí),可利用電荷量檢測(cè)廢水BOD濃度。以硝酸酸化處理的石墨氈為電極材料,H2O2為陰極電子受體構(gòu)建的BOD傳感器的最佳檢測(cè)條件為：廢水中L-半胱氨酸投加量為20 mg/L、緩沖溶液投加量為50mL/L。H2O2濃度為0.5mmol/L時(shí),BOD濃度檢測(cè)范圍為2-200 mg/L;H2O2濃度為1.0 mmol/L時(shí),BOD濃度檢測(cè)范圍為2～300mg/L;H2O2濃度為1.5mmol/L時(shí),BOD濃度檢測(cè)范圍為2～500 mg/L。三種H202濃度條件下,在各自的檢測(cè)范圍內(nèi),響應(yīng)時(shí)間均小于4h。通過(guò)在陰極液中投加叔丁醇間接證明了H2O2在運(yùn)行過(guò)程中分解生成了·OH,且·OH參與了陰極反應(yīng)。選擇硝酸酸化的石墨氈作為電極材料,H2O2作陰極電子受體以達(dá)到快速準(zhǔn)確檢測(cè)的目的。在廢水配方中加入面粉作為懸浮可降解有機(jī)物代表和廢水中的可溶性可降解有機(jī)物葡萄糖可被陽(yáng)極上的微生物菌群作為底物降解,增大了產(chǎn)電電流,延長(zhǎng)了響應(yīng)時(shí)間。利用PCR-DGGE技術(shù)進(jìn)行了陽(yáng)極表面微生物多樣性的分析,陽(yáng)極上富集的微生物菌落由8種產(chǎn)電菌和8種非產(chǎn)電菌組成,兩類(lèi)菌種互營(yíng)共生。
[Abstract]:Biochemical oxygen demand (bod) is a comprehensive indicator of organic pollution. Traditional five day biochemical oxygen demand (BOD5) is the standard method for detecting bod concentration, but it has some defects such as long time consuming and poor reproducibility. Microbial fuel cell (MFC) bod sensor is a new method for rapid detection of BOD5, which has the advantages of strong stability and wide detection range. In this paper, using anaerobic sludge as inoculum and proton exchange membrane as the separator of cathode and cathode cell, we have constructed a double compartment MFC bod sensor without medium, and optimized anode material and cathode electron receptor. After improving the detection performance of MFC type bod sensor, the feasibility of MFC type bod sensor for detecting actual wastewater was preliminarily studied by simulating the actual wastewater, and the microbial community structure of anode was analyzed by PCR-DGGE. Graphite felt electrode was modified by nitric acid acidizing. The surface contact angle of graphite felt changed greatly when it was acidified for 4 h. Using graphite felt treated by nitric acid as electrode material and dissolved oxygen as cathode electron receptor, the optimal detection conditions of MFC bod sensor are as follows: the flow rate of cathode influent is 30 mL / min, the dosage of buffer solution is 50 mL / L / L, the dosage of cysteine is 20 mg / L; The detection range of external resistance is 500 惟 路bod, the response time is less than 10 h, the data reproducibility is good, the concentration of bod is in the range of 2-50 mg / L, the maximum current can be used to detect the concentration of bod in waste water in the range of 2 ~ 100 mg / L, The concentration of bod in wastewater can be measured by charge quantity. An MFC bod sensor was constructed using carbon brush as electrode material and dissolved oxygen as cathode electron receptor. The detection range of bod concentration is 2 ~ 100 mg / L, the response time is less than 12 h, the data reproducibility is good. The concentration of bod is in the range of 2-60 mg / L, and the peak current can be used to detect the bod concentration of wastewater. When bod concentration is in the range of 2 ~ 100 mg / L, bod concentration can be measured by charge quantity. The best detection conditions of bod sensor with graphite felt treated by nitric acid as electrode material and H _ 2O _ 2 as cathode electron receptor are as follows: the dosage of L-cysteine in wastewater is 20 mg / L, the dosage of buffer solution is 50 mL / L and the concentration of H _ 2O _ 2 is 0.5 mmol 路L ~ (-1). When the concentration of H _ 2O _ 2 was 1.0 mmol / L, the detection range of bod was 2 ~ 300 mg / L ~ (-1) mg 路L ~ (-1) H _ 2O _ 2 was 1.5 mmol / L ~ (-1) mg 路L ~ (-1) 路L ~ (-1) H _ 2O _ 2 was 2 500 mg 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) of H _ 2O _ 2. The response time was less than 4 h in each detection range under three H202 concentrations. By adding tert-butanol to the cathode solution, it was proved indirectly that H _ 2O _ 2 was decomposed into OH and OH was involved in the cathodic reaction. Hydrogen peroxide (H _ 2O _ 2) was used as cathode electron receptor for rapid and accurate detection of nitrate-acidified graphite felt. Adding flour as the representative of suspended degradable organic matter and soluble degradable organic glucose in wastewater as substrate can be degraded by microbial flora on the anode, which increases the electric current and prolongs the response time. The microbial diversity on the anode surface was analyzed by PCR-DGGE technique. The enriched microorganism colonies on the anode were composed of 8 electro producing bacteria and 8 non electro producing bacteria, and the two species were symbiotic with each other.
【學(xué)位授予單位】：北京林業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：TM911.4;TP212

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