本文選題：聚羥基丁酸戊酸酯 + 多孔膜　； 參考：《蘭州理工大學》2017年碩士論文

【摘要】：石油基高分子材料廣泛的應用于各個領(lǐng)域,對人類的生活和國民的經(jīng)濟產(chǎn)生著重要作用,但隨著能源材料危機的不斷加深以及白色污染的加劇,可持續(xù)發(fā)展的生物基高分子材料便備受青睞。聚羥基脂肪酸酯(Polyhydroxyalkanoats,PHA)是惟一一個完全由微生物合成的生物降解基的高分子材料。同時PHA相對于其他生物材料(聚乳酸PLA、丁二酸丁二醇共聚物PBS、聚羥基乙酸PGA、淀粉基塑料等)結(jié)構(gòu)多元化,種類繁多,物化性能不盡相同,可以適用于不同條件下的應用要求,PHA已成為生物材料領(lǐng)域最為活躍的研究熱點之一。高分子超濾膜已在顆粒脫除、蛋白等物質(zhì)的分離與純化、水質(zhì)凈化、污水處理、血液透析和細胞多孔支架等眾多領(lǐng)域得到廣泛應用。可制備超濾膜的高分子大部分為石油基高分子材料,膜材料依賴與石油能源,而且廢棄后不具備生物降解性能,易對環(huán)境造成新的污染,因此開發(fā)源于可再生資源并具備生物可降解性的新型高分子多孔膜材料日益受到關(guān)注,此外具有生物相容性的多孔膜在生物醫(yī)用領(lǐng)域應用日益收到重視。本文以二甲基乙酰胺(DMAc)作為溶劑,采用相轉(zhuǎn)化法(NIPS)制備聚羥基丁酸戊酸酯(PHBV)平板多孔膜,通過對非溶劑、鑄膜液濃度、凝固溫度、凝固浴組成的調(diào)節(jié)、添加劑等,調(diào)控成膜過程中的相分離行為,制備出結(jié)構(gòu)可控的PHBV多孔膜。并用生物質(zhì)聚羥基丁酸戊酸酯多孔膜作為細胞培養(yǎng)的培養(yǎng)基質(zhì),培養(yǎng)Hela細胞,觀察細胞生長狀況,探究聚羥基丁酸戊酸酯多孔膜的生物相容性。研究表明,45℃時PHBV/DMAc/H_2O體系分相比PHBV/DMAc/乙醇體系更快。當凝固浴為純水時,非溶劑和溶劑交換速率快,發(fā)生瞬時分相,而PHBV固化速率較低,多孔膜形貌呈指狀孔、海綿孔并存。隨著凝固浴中溶劑DMAc的加入,非溶劑和溶劑的交換速率變緩,從而發(fā)生延時分相,多孔膜呈均勻的海綿狀結(jié)構(gòu)。添加劑中聚乙烯吡咯烷酮PVP對多孔膜的成孔效果最佳,不僅有利于多孔膜的形成,而且下表面形成排列規(guī)整的蜂窩狀孔,蜂窩狀孔內(nèi)有小孔存在,孔徑分布比較均勻。親水物質(zhì)PVP的加入,也是多孔膜的水通量得到提高。在浸潤溫度為80℃時,凝固浴中為純水時,相分離過程中,熱力學與動力學相互作用,使PHBV/PVP多孔膜的成孔效果好,孔隙率和水通量相對較高。用PHBV多孔膜作為細胞培養(yǎng)基質(zhì),對Hela細胞進行細胞培養(yǎng),不同細胞培養(yǎng)基質(zhì),Hela細胞的Viability值在90%以上,證明PHBV多孔膜無毒性,細胞可以在上面進行生長,不會對細胞產(chǎn)出影響。并通過DAPI染劑對細胞進行染色,在熒光顯微鏡下觀察到,蜂窩狀PHBV多孔膜作為細胞培養(yǎng)基質(zhì)時,Hela細胞生長、繁殖情況好。因此PHBV多孔膜為生物相容性好,可以應用在生物醫(yī)學領(lǐng)域。
[Abstract]:Petroleum based polymer materials are widely used in various fields, which play an important role in human life and national economy. However, with the deepening of the crisis of energy materials and the intensification of white pollution,Sustainable development of biological-based polymer materials will be favored.Polyhydroxyalkanoate (PHA) is the only biodegradable polymer material completely synthesized by microorganisms.At the same time, compared with other biomaterials (polylactic acid (PLA), butylene succinate copolymer (PHA), polyglycolic acid (PGA), starch based plastics, etc.), the structure of PHA is various, and its physical and chemical properties are different.PHA, which can be applied to different conditions, has become one of the most active research hotspots in the field of biomaterials.Polymer ultrafiltration membrane has been widely used in many fields, such as particle removal, protein separation and purification, water purification, sewage treatment, hemodialysis and cellular porous scaffolds.Most of the polymers that can be prepared by ultrafiltration membrane are petroleum-based polymer materials. The membrane materials depend on petroleum energy and do not have biodegradability after being abandoned, which is easy to cause new pollution to the environment.Therefore, the development of new polymeric porous membrane materials with biodegradability and renewable resources has attracted more and more attention. In addition, the application of biocompatible porous membrane in biomedical field has been paid more and more attention.In this paper, polyhydroxybutyrate valerate (PHBV) flat porous membranes were prepared by phase inversion method (NIPS) using dimethyl acetamide (DMAC) as solvent. The effects of non-solvent, concentration of casting solution, solidification temperature, composition of coagulation bath, additives, etc.PHBV porous membranes with controllable structure were prepared by regulating the phase separation behavior during the process of membrane formation.Biomass polyhydroxybutyrate valerate porous membrane was used as the culture matrix of cell culture to culture Hela cells, observe the cell growth, and explore the biocompatibility of polyhydroxybutyrate polyvalerate porous membrane.The results show that PHBV/DMAc/H_2O system is faster than PHBV/DMAc/ ethanol system at 45 鈩，

本文編號：1734160