本文選題：電沉積技術(shù)　切入點：殼聚糖　出處：《武漢理工大學》2015年碩士論文

【摘要】：微電子技術(shù)和生物技術(shù)是20世紀發(fā)展最為迅速的兩大技術(shù),進入21世紀人們迫切希望將這兩種技術(shù)結(jié)合起來進行應用。近年來,研究者通過結(jié)合微電子技術(shù)與生物技術(shù),對一些電刺激響應性生物大分子的電沉積技術(shù)開展了研究,其中殼聚糖的電沉積技術(shù)研究受到了廣泛關(guān)注,殼聚糖的電沉積技術(shù)具有操作簡單、條件溫和、時間和空間選擇性和可控性等性能特點和優(yōu)點,被廣泛應用于生物傳感器、藥物控制釋放、儲能材料、生物燃料電池等領域。本工作開展了殼聚糖與量子點電沉積技術(shù)的研究,還開展了殼聚糖與配位金屬離子電沉積技術(shù)的研究。量子點(QDs)呈現(xiàn)出許多特殊的物理和化學性能,被廣泛應用于生物檢測、生物成像、生物傳感器、熒光圖案等領域。本工作中我們開展了殼聚糖與Zn S量子點電沉積技術(shù)的研究,首先制備了一種水性ZnS QDs,再利用電沉積技術(shù)實現(xiàn)了殼聚糖/ZnS量子點沉積膜的制備,研究了電沉積條件對制備的殼聚糖/ZnS量子點沉積膜熒光強度的影響,然后采用不同形狀的電極及保護性涂層的方法進行了殼聚糖/Zn S量子點熒光圖案的制備,以及熒光圖案的消除與重復性再現(xiàn)。研究結(jié)果表明利用電沉積技術(shù)能夠?qū)崿F(xiàn)在不同電極(金、銀、鈦及316L不銹鋼)上制備殼聚糖/ZnS量子點沉積膜,且制備的殼聚糖/ZnS量子點沉積膜具有明顯的藍色熒光,并且沉積膜的熒光強度可以通過改變電沉積條件進行控制;此外,利用電沉積技術(shù)能夠?qū)崿F(xiàn)在不同類型的電極或基材上制備殼聚糖/ZnS量子點熒光圖案,該熒光圖案可以消除和重復性再現(xiàn),該方法在構(gòu)建光電子器件、熒光涂層和圖案、生物電子器件的等領域具有潛在的應用價值。殼聚糖分子可以與配位金屬離子通過配位作用生成凝膠,本工作利用金屬銅作為陽極,利用電沉積技術(shù)實現(xiàn)了殼聚糖/Cu2+水凝膠的制備,經(jīng)過一系列的實驗對殼聚糖/Cu2+水凝膠電沉積機理進行了驗證,研究了電沉積條件(沉積時間、沉積電壓)對殼聚糖/Cu2+電沉積的影響。研究結(jié)果顯示采用金屬銅作為陽極,利用電沉積技術(shù)能夠?qū)崿F(xiàn)殼聚糖/Cu2+水凝膠的制備,制備的殼聚糖/Cu2+水凝膠表面平整、無氣泡,呈現(xiàn)了與Cu2+顏色相同的藍色,殼聚糖/配位金屬離子水凝膠的厚度可以通過調(diào)節(jié)電沉積條件進行控制;該殼聚糖/配位金屬離子的電沉積技術(shù)既具有常規(guī)陰極電沉積技術(shù)的時間和空間選擇性和可控性,又具有配位金屬離子的選擇性。因此,該殼聚糖/配位金屬離子的電沉積不僅為殼聚糖的電沉積技術(shù)提供了一種新型、簡單、可控的方法,而且制備的殼聚糖/配位金屬離子凝膠在生物電子器件、固定蛋白質(zhì)、表面涂層及圖案化、藥物控制釋放等領域具有潛在的應用價值。
[Abstract]:Microelectronics and biotechnology are the two most rapidly developing technologies in the 20th century. In the 21st century, people are eager to combine these two technologies for application.In recent years, researchers have carried out research on electrodeposition of some responsive biomolecules by combining microelectronics and biotechnology, among which the research of chitosan electrodeposition has been paid more and more attention.Chitosan electrodeposition technology has been widely used in biosensor, controlled drug release, energy storage materials, biofuel cells and other fields because of its simple operation, mild conditions, time and space selectivity and controllability.In this work, the electrodeposition of chitosan and QDs was carried out, and the electrodeposition of chitosan and coordination metal ions was also studied.Quantum Dots (QDs) show many special physical and chemical properties, which are widely used in the fields of biological detection, biological imaging, biosensor, fluorescence patterns and so on.In this work, we studied the electrodeposition of chitosan and ZnS quantum dots. Firstly, we prepared a kind of water-borne ZnS QDs, and then we realized the preparation of chitosan / ZnS quantum dot films by electrodeposition.The effect of electrodeposition conditions on the fluorescence intensity of the prepared chitosan / ZnS quantum dots was studied. Then the fluorescence patterns of chitosan / ZnS quantum dots were prepared by using different electrodes and protective coatings.And the elimination of fluorescent patterns and repetitive reproduction.The results show that chitosan / ZnS quantum dot films can be deposited on different electrodes (gold, silver, titanium and 316L stainless steel) by electrodeposition.Moreover, the fluorescence intensity of the deposited films can be controlled by changing the electrodeposition conditions. In addition, the fluorescence patterns of chitosan / ZnS quantum dots can be prepared on different types of electrodes or substrates by electrodeposition.The fluorescence pattern can be eliminated and reproduced repeatedly. This method has potential application value in the construction of optoelectronic devices, fluorescent coatings and patterns, bioelectronic devices and so on.Chitosan molecules can coordinate with coordination metal ions to form gels. In this work, the preparation of chitosan / Cu _ 2 hydrogels was realized by electrodeposition using metal copper as anode.The electrodeposition mechanism of chitosan / Cu _ 2 hydrogel was verified by a series of experiments. The effect of electrodeposition conditions (deposition time, deposition voltage) on the electrodeposition of chitosan / Cu _ 2 was studied.The results show that the preparation of chitosan / Cu _ 2 hydrogel can be realized by using metal copper as anode and electrodeposition technology. The prepared chitosan / Cu _ 2 hydrogel has a flat surface and no bubble, showing the same blue color as Cu2.The thickness of chitosan / coordination metal ion hydrogel can be controlled by adjusting the electrodeposition conditions, and the electrodeposition of the chitosan / coordination metal ion has the temporal and spatial selectivity and controllability of conventional cathodic electrodeposition.It also has the selectivity of coordination metal ions.Therefore, the electrodeposition of the chitosan / coordination metal ions not only provides a new, simple and controllable method for the electrodeposition of chitosan, but also makes the chitosan / coordination metal ion gel in bioelectronic devices.The fields of fixed protein, surface coating and patterning, drug controlled release have potential application value.
【學位授予單位】：武漢理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TQ153;O636.1

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