HPLC測定不同煎煮方法小柴胡湯中9種成分的含量
發(fā)布時間:2019-08-24 19:13
【摘要】:目的:考察去滓再煎法、不去滓煎法、兩次煎法3種煎煮方法對小柴胡湯中9種成分含量的影響。方法:采用Welch Ultimate XB-C_(18)色譜柱(4.6 mm×150 mm,5μm),流動相乙腈-0.1%磷酸溶液,梯度洗脫,柱溫26℃,流速1.0 m L·min~(-1),檢測波長203,252,280 nm。結(jié)果:去滓再煎法中柴胡皂苷a的含量比不去滓煎法、現(xiàn)代兩次煎法分別提高了62.41%,49.01%,柴胡皂苷d的含量比其他2種煎法分別提高了59.47%,28.71%,柴胡皂苷b1的含量比其他2種煎法分別提高了41.93%,16.14%。去滓再煎法中人參皂苷Re的含量比不去滓煎法提高了33.96%,比兩次煎法提高了0.20%。去滓再煎法中黃芩苷,人參皂苷Rb1,甘草酸單銨鹽,甘草苷4種成分的含量略低于兩次煎法,高于不去滓煎法。不去滓煎法中各種成分的含量是3種煎法中最低的(6-姜辣素除外)。結(jié)論:該結(jié)果可有效反映不同煎煮方法的質(zhì)量差異。在3種煎法中,去滓再煎的方法明顯提高了柴胡在水煎液中有效成分的溶出,從一定程度上提升了小柴胡湯的臨床功效,為張仲景《傷寒論》中對小柴胡湯采用去滓再煎的方法及質(zhì)量評價提供了良好的實驗依據(jù)。
[Abstract]:Objective: to investigate the effect of three decoction methods on the content of 9 components in Xiaochaihu decoction. Methods: a Welch Ultimate XB-C_ (18) column (4.6 mm 脳 150 mm, 5 渭 m) was used, the mobile phase was acetonitrile-0.1% phosphate solution, gradient eluted, the column temperature was 26 鈩,
本文編號:2529128
[Abstract]:Objective: to investigate the effect of three decoction methods on the content of 9 components in Xiaochaihu decoction. Methods: a Welch Ultimate XB-C_ (18) column (4.6 mm 脳 150 mm, 5 渭 m) was used, the mobile phase was acetonitrile-0.1% phosphate solution, gradient eluted, the column temperature was 26 鈩,
本文編號:2529128
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