發(fā)布時(shí)間：2019-07-10 11:40

【摘要】：本文采用納米Fe_3O_4顆粒作為磁性核心,先用四乙氧基硅烷、再用3-巰丙基三乙氧基硅烷和3-氨丙基三乙氧基硅烷修飾Fe_3O_4顆粒,形成表面帶-NH_2和-SH的Fe_3O_4/SiO_2納米顆粒,進(jìn)一步通過-NH_2的靜電吸附和Au-S鍵的作用將金納米顆粒組裝在Fe_3O_4/SiO_2表面,形成具有核殼結(jié)構(gòu)的Fe_3O_4/SiO_2/Au金磁納米顆粒,并用透射電子顯微鏡鏡(TEM)、能量色散X射線光譜儀(EDX)、紫外可見分光光度計(jì)(UV-vis)等技術(shù)對金磁納米顆粒進(jìn)行了形貌觀測及性質(zhì)表征。利用Fe_3O_4/SiO_2/Au金磁納米顆粒作為拉曼活性基底,用表面增強(qiáng)拉曼光譜儀對黃曲霉毒素B1(AFB1)進(jìn)行直接快速檢測,發(fā)現(xiàn)無外磁體濃縮的情況下AFB1的檢測限大于10.0μg/m L,在外磁體濃縮金磁納米顆粒的情況下檢測限降低100倍(≤0.1μg/m L),檢測線性范圍0.1μg/m L~10.0μg/m L,檢測的樣品回收率為84.35%~91.98%,相對標(biāo)準(zhǔn)偏差在4.88%~9.90%之間。
[Abstract]:In this paper, nano-Fe_3O_4 particles were used as magnetic core, first with tetraethoxysilane, then modified with 3-mercaptopropyltriethoxysilane and 3-aminopropyl triethoxysilane to form Fe_3O_4/SiO_2 nanoparticles with-NH_2 and-SH on the surface. Furthermore, the gold nanoparticles were assembled on the surface of Fe_3O_4/SiO_2 by electrostatic adsorption of-NH_2 and Au-S bond, and Fe_3O_4/SiO_2/Au gold magnetic nanoparticles with core-shell structure were formed. The (TEM), energy dispersive X-ray spectrometer (EDX), was used as transmission electron microscope. The morphology and properties of gold magnetic nanoparticles were observed by UV-vis spectrophotometer (UV-vis). Using Fe_3O_4/SiO_2/Au gold magnetic nanoparticles as Raman active substrate, aflatoxin B1 (AFB1) was detected directly and rapidly by surface enhanced Raman spectrometer. It was found that the detection limit of AFB1 was more than 10.0 渭 g / mL in the absence of external magnet concentration, and the detection limit was reduced by 100 times (鈮，

本文編號：2512577